Human Flexor Tendon Tissue Engineering: Decellularization of Human Flexor Tendons Reduces Immunogenicity <i>In Vivo</i>

Raghavan, Shyam S; Woon, Colin Yi-Loong; Kraus, Armin; Megerle, Kai; Choi, Matthew; Pridgen, Brian C.; Pham, Hung; Chang, James

doi:10.1089/ten.tea.2011.0422

Cited by 65 publications

(58 citation statements)

References 38 publications

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“…This should be suitable for allo-and xenogeneic transplantation, which would allow unobstructed host cell immigration and proliferation, and would optimally even stimulate their differentiation [Schulze-Tanzil et al, 2012]. Decellularization has been successfully applied to a variety of tissues amongst them musculoskeletal tissue samples of various sizes, such as skeletal muscle [Borschel et al, 2004] and tendons [Pridgen et al, 2011;Raghavan et al, 2012;Bronstein et al, 2013].…”

Section: Discussionmentioning

confidence: 99%

Establishment of a Cytocompatible Cell-Free Intervertebral Disc Matrix for Chondrogenesis with Human Bone Marrow-Derived Mesenchymal Stromal Cells

Huang¹,

Kohl²,

Kokozidou³

et al. 2016

Cells Tissues Organs

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Tissue-engineered intervertebral discs (IVDs) utilizing decellularized extracellular matrix (ECM) could be an option for the reconstruction of impaired IVDs due to degeneration or injury. The objective of this study was to prepare a cell-free decellularized human IVD scaffold and to compare neotissue formation in response to recellularization with human IVD cells (hIVDCs) or human bone marrow-derived (hBM) mesenchymal stromal cells (MSCs). IVDs were decellularized via freeze-thaw cycles, detergents and trypsin. Histological staining was performed to monitor cell removal and glycosaminoglycan (GAG) removal. The decellularized IVD was preconditioned using bovine serum albumin and fetal bovine serum before its cytocompatibility for dynamically cultured hBM-MSCs (chondrogenically induced or not) and hIVDCs was compared after 14 days. In addition, DNA, total collagen and GAG contents were assessed. The decellularization protocol achieved maximal cell removal, with only few remaining cell nuclei compared with native tissue, and low toxicity. The DNA content was significantly higher in scaffolds seeded with hIVDCs compared with native IVDs, cell-free and hBM-MSC-seeded scaffolds (p < 0.01). The GAG content in the native tissue was significantly higher compared to the others groups except for the scaffolds reseeded with chondrogenically induced hBM-MSCs (p < 0.05). In addition, there was a significantly increased total collagen content in the chondrogenically induced hBM-MSCs group (p < 0.01) compared with the native IVDs, cell-free and hIVDC-seeded scaffolds (p < 0.01); both recolonizing cell types were more evenly distributed on the scaffold surface, but only few cells penetrated the scaffold. The resulting decellularized ECM was cytocompatible and allowed hBM-MSCs/hIVDCs survival and ECM production.

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Section: Discussionmentioning

confidence: 99%

Establishment of a Cytocompatible Cell-Free Intervertebral Disc Matrix for Chondrogenesis with Human Bone Marrow-Derived Mesenchymal Stromal Cells

Huang¹,

Kohl²,

Kokozidou³

et al. 2016

Cells Tissues Organs

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“…10 The ideal scaffold should not only be biocompatible and match the natural biomechanical properties, but should also comprise naturally structured, extracellular matrix proteins that can interact with the repopulating cells and direct them toward tenogenic differentiation and matrix remodeling. 10,11 Decellularization of tendon tissue offers the unique opportunity of obtaining a scaffold with a natural extracellular matrix structure that is hypoimmunogenic 13,14 and displays biomechanical properties very similar to the original tissue, [15][16][17][18] providing great advantages for potential clinical application and research use.…”

Section: Introductionmentioning

confidence: 99%

Freeze-Thaw Cycles Enhance Decellularization of Large Tendons

Burk¹,

Erbe²,

Berner³

et al. 2014

Tissue Engineering Part C: Methods

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“…[6][7][8] We have also shown improved ability to reseed these grafts with fibroblasts after peracetic acid treatment. 9 Biomechanical testing demonstrated no significant difference in elastic modulus or tensile strength after peracetic acid treatment.…”

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confidence: 80%