Human fetal skin-derived stem cell secretome enhances radiation-induced skin injury therapeutic effects by promoting angiogenesis

Abstract

BackgroundRadiation dermatitis is a refractory skin injury caused by radiotherapy. Human fetal skin-derived stem cell (hFSSC) is a preferable source for cell therapy and skin tissue regeneration. In the present study, we investigated the repair effect of using hFSSC secretome on a radiation skin injury model in rats.MethodsWe prepared the hFSSC secretome and studied its effects on the proliferation and tube formation of human umbilical vein endothelial cell (HUVEC) in vitro. Furthermore, we used a Sr-90 radiation-induced skin injury model of rats and evaluated the effects of hFSSC secretome on radiation skin injury in vivo.ResultsThe results showed that hFSSC secretome significantly promoted the proliferation and tube formation of HUVEC in vitro; in addition, hFSSC secretome-treated rats exhibited higher healing quality and faster healing rate than the other two control groups; the expression level of collagen type III α 1 (Col3A1), transforming growth factor β3 (TGF-β3), angiotensin 1 (Ang-1), angiotensin 2 (Ang-2), vascular endothelial growth factor (VEGF), and placental growth factor (PLGF) was significantly increased, while collagen type I α 2 (Col1A2) and transforming growth factor β1 (TGF-β1) were decreased in hFSSC secretome group.ConclusionsIn conclusion, our results provided the first evidence on the effects of hFSSC secretome towards radiation-induced skin injury. We found that hFSSC secretome significantly enhanced radiation dermatitis angiogenesis, and the therapeutic effects could match with the characteristics of fetal skin. It may act as a kind of novel cell-free therapeutic approach for radiation-induced cutaneous wound healing.

Anti-fibrotic effect of human fetal skin-derived stem cell secretomeon the liver fibrosis

“…Moreover, hFSSCs secretome treatment is more effective than of hUCMSCs secretome in resolving brosis, such as some indicators included collagen area, MDA, Hyp, TBIL, and γ-GT. The reasons for the differences in the result between hUCMSCs, and hFSSCs secretome may be due to the difference in the presence more biological activity factors contain in hFSSCs secretome than hUCMSCs secretome [13]. Our studies indicated that hFSSC secretome is an attractive emerging option for therapeutic applications as a therapeutic strategy for liver brosis.…”

“…Recently, fetal mesenchymal stem cells functional secretome analysis illustrates that 737±80 protein identi cations were obtained from the amniotic uid-mesenchymal stem cells (AF-MSCs) secretome, interestingly, it reveals that Annexin-A1 as an important paracrine factor in hepatic regeneration [22]. However, the previous study demonstrated that hFSSC presented with the characteristics of both MSC and embryonic stem cell (ESC) [13]. These may be the partial reason that hFSSC secretome can effectively reduce liver brosis, and has better effects than hUCMSC secretome in some indicators levels.…”

“…Cell culture hFSSCs and human umbilical cord mesenchymal stem cells (hUCMSCs) were provided and isolated by following our previous study [13]. HSCs were purchased from the Chinese Academy of Medical Sciences, China.…”

“…In brief, hFSSCs, hUCMSCs, and HSCs were cultured in DMEM (Gibco, Grand island, U.S.) supplemented with 500 U/ml penicillin and 500 μg/ml streptomycin (Invitrogen, Shanghai, China), and 10% FBS (Gibco, Grand island, U.S.) at 37°C, with saturated humidity and 5% CO 2 . hFSSCs and hUCMSCs at the P5 were used for this study, and hFSSCs and hUCMSCs) secretome was collected as reported in our previous study [13]. Brie y, cells were cultured and reached 70~80% con uence, placed in serum-free medium (SFM; Invitrogen, Shanghai, China), and incubated in 5% CO 2 in a humidi ed condition.…”

“…In our previous study, we have successfully isolated and characterized hFSSCs therapeutic quality control [13]. Interestingly, we found that hFSSC secretome has a great ability to control and balance the collagen formation in skin tissue [13].…”