Human erythrocyte metabolism studies by ¹H spin echo NMR

UPLC-based metabonomic applications for discovering biomarkers of diseases in clinical chemistry

“…An increasing number of publications have described metabonomics using analytical techniques including 1 H NMR, gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (UPLC-MS) [6]. 1 H NMR, which is as one of the first methods used for metabonomics, represents a rapid, non-destructive and highly robust technology that provide highly informative structural information [7]. 1 H NMR is often used without any pre-separation process and unlike chromatography it does not require development.…”

6‐Fluoropyridoxol: A novel probe of cellular pH using 19F NMR spectroscopy

“…NMR offers several potential advantages: continuous non-destructive monitoring of pH in vivo; spatial resolution and simultaneous determination of pH in several compartments [4,5]. NMR has been used to monitor titratable groups of endogenous metabolites on the basis of chemical shift with respect to changing pH; in particular 3'P NMR of inorganic phosphate (Pi) [5,6] or phosphomonoesters [7] or 'H NMR of carnosine [8] or histidine [9]. pH measurement using endogenous metabolites is not always successful owing to crowded spectra, broad lines or low metabolite concentration.…”

The incorporation of 2h‐labelled glycine into the glutathione of intact human erythrocytes studied by 1h spin‐echo fourier transform NMR

“…Although the concentration of glutathione (y-Lglutamyl-L-cysteinyl-glycine, GSH) in human erythrocytes is generally found to be -2 mM [ 1,2], it is in a dynamic state, continually being degraded to and resynthesized from its constituent amino acids. The details of the degradation and synthesis reactions are the subject of considerable research [3,4], as is the mechanism by which the GSH level is regulated [l].…”