Human embryonic stem cells and metastatic colorectal cancer cells shared the common endogenous human microRNA-26b

Abstract

The increase in proliferation and the lack of differentiation of cancer cells resemble what occur in the embryonic stem cells during physiological process of embryogenesis. There are also striking similarities in the behaviour between the invasive placental cells and invasive cancer cells. In the present study, microarrays were used to analyse the global expression of microRNAs in a human embryonic stem cell line (i.e. HUES-17) and four colorectal cancer (CRC) cell lines (i.e. LoVo, SW480, HT29 and Caco-2) with different metastatic potentialities. Only the expression of miR-26b was significant decreased in HUES-17s and LoVo cells, compared with other three cell lines (P < 0.01). The quantitative real-time PCR analysis confirmed the results of the microarray analysis. Overexpression of miR-26b expression by miR-26 mimics transfection and led to the significant suppression of the cell growth and the induction of apoptosis in LoVo cells in vitro, and the inhibition of tumour growth in vivo. Moreover, the potential targets of miR-26b was predicted by using bioinformatics, and then the predicted target genes were further validated by comparing gene expression profiles between LoVo and NCM460 cell lines. Four genes (TAF12, PTP4A1, CHFR and ALS2CR2) with intersection were found to be the targets of miR-26b. MetaCore network analysis further showed that the regulatory pathways of miR-26b were significantly associated with the invasiveness and metastasis of CRC cells. These data suggest that miR-26b might serve as a novel prognostic factor and a potential therapeutic target for CRC.

Elevated oncofoetal miR-17-5p expression regulates colorectal cancer progression by repressing its target gene P130

“…As reported in our previous studies of a potential oncofoetal CRC biomarker, certain high-value biomarkers, including miRNAs, genes and cancer proteins, show embryonic characteristics [10][11][12][13] . Tumorigenesis and embryogenesis have been postulated to share certain common pathways 10 .…”

Dysregulation of global microRNA expression in splenic marginal zone lymphoma and influence of chronic hepatitis C virus infection

“…7,48,49 The diminished expression of miR-26b, as seen in the HCV-positive lymphoma group, has been linked to a malignant tumor phenotype in hepatocellular carcinoma 50 and lung carcinoma 51 as well as in cell culture models of colon cancer and diffuse large B-cell lymphoma. 52,53 Moreover, overexpression of miR-26b in colon cancer cell lines led to significant suppression of cell growth and the induction of apoptosis. 53 Epidemiological data clearly link HCV infection with a higher risk of lymphoma development.…”

“…52,53 Moreover, overexpression of miR-26b in colon cancer cell lines led to significant suppression of cell growth and the induction of apoptosis. 53 Epidemiological data clearly link HCV infection with a higher risk of lymphoma development. 54,55 Moreover, eradication of the virus by antiviral therapy can lead to lymphoma remission, which points to a causal role of HCV in malignant transformation, as reviewed by Arcaini et al 15 SMZL is one of the subentities of B-cell nonHodgkin lymphoma found associated with HCV infection in a statistically significant manner.…”

Intronic miR-26b controls neuronal differentiation by repressing its host transcript,ctdsp2

“…Additionally, large-scale analysis revealed a miR-26 target site in the Ctdsp2 39 UTR that is bound by a miRNA silencing complex in the mouse brain (Chi et al 2009). Finally, the regulated feedback by miR-26a and miR-26b might be important not only in neurons, but for proliferation/differentiation events in general, as both family members have been implicated with differentiation of other cell types, stemness, and cancer (Wong and Tellam 2008;Huse et al 2009;Kota et al 2009;Ma et al 2010). …”