Human DNA topoisomerase‐IIα expression as a prognostic factor for transitional cell carcinoma of the urinary bladder

Abstract

Objective To investigate the immunohistochemical expression of topoisomerase II‐α (TII‐α, a nuclear enzyme, the expression of which increases rapidly at the end of the S to G2/M phase and declines when mitosis ends) in bladder urothelial neoplasms (transitional cell carcinoma), and its correlation with grade, stage and survival. patients and methods Histological sections from 57 urothelial neoplasms were stained immunohistochemically for TII‐α expression; the percentage of positive cells in the area of greatest staining was recorded as the TII‐α index. Results TII‐α nuclear staining was positive in all the samples except one. The mean (sd) TII‐α index was 10.7 (5.9) in urothelial neoplasms of low malignant potential (grade 1), 15.5 (5.0) in low‐grade (grade 2) and 42.1 (13.4) in high‐grade urothelial carcinoma (grade 3). The mean TII‐α index was 10.7 (5.9), 26.3 (14.8) and 44 (19) in stages pTa, pT1 and pT2, respectively. The TII‐α index was significant for predicting death from cancer, independently of the stage or grade of the disease (P = 0.019, hazard ratio 1.1). Conclusions A higher TII‐α index indicates a greater probability of recurrence of disease and lower overall survival. Therefore TII‐α expression has prognostic value in bladder carcinoma.

The Role of Phenethyl Isothiocyanate on Bladder Cancer ADM Resistance Reversal and Its Molecular Mechanism

“…When used the chemotherapy drugs, chemotherapy drugs could catch aggregates, make the non‐cocovalently bound to covalently bound. If Top‐DNA aggregates reduced, it would lead to the emergence of drug resistance (Koren et al, ). Some reports considered that isothiocyanate could develop the cancer chemopreventive activity through inhibiting to phase I enzymes cytochrome P‐450s, such as glutathione S transferases (GST) (Wu et al, ).…”

Expression of Multidrug Resistance-Related Protein (Mrp-1), Lung Resistance-Related Protein (Lrp) and Topoisomerase-Ii (Topo-Ii) in Wilms' Tumor: Immunohistochemical Study Using Tma Methodology

“…Kong et al 8 reported that LRP was upregulated in urothelial carcinomas, while MRP1 showed no similar extent of overexpression in these tumors. Koren et al 9 reported that Topo-II nuclear staining was positive in almost all transitional cell carcinoma samples studied and suggested that its expression could be thus of prognostic value in bladder carcinoma. The modern multi-modality therapeutic approach to WT, combining surgery with radiotherapy and chemotherapy, results in high cure rates even in advanced stage disease, with a pivotal role attributed to chemotherapy.…”

Neutral dimeric copper–sparfloxacin conjugate having butterfly motif with antiproliferative effects against hormone independent BT20 breast cancer cell line

“…breast cancer, testicular teratoma and transitional cell carcinomas [15]. Several studies indicate that the sensitivity to the topoIIa-inhibitors is directly dependent on the expression level of topoIIa in cancer cells [16]. Recently high expression of topoIIa in breast cancer has been correlated with hormone independent pathway [17].…”