Human Dna Quantification in the Stools of Patients With Colorectal Cancer

Abstract

-Background -Colorectal cancer is one of the main cause of cancer in the world. Colonoscopy is the best screen method, however the compliance is less than 50%. Quantification of human DNA (hDNA) in the feces may be a possible screen non-invasive method that is a consequence of the high proliferation and exfoliation of cancer cells. Objective -To quantify the human DNA in the stools of patients with colorectal cancer or polyps. Methods -Fifty patients with CRC, 26 polyps and 53 with normal colonoscopy were included. Total and human DNA were analyzed from the frozen stools. Results -An increased concentration of hDNA in the stools was observed in colorectal cancer patients compared to controls and polyps. Tumors localized in the left side of the colon had higher concentrations of hDNA. There were no difference between polyps and controls. A cut off of 0.87 ng/mL of human DNA was determined for colorectal cancer patients by the ROC curve, with a sensitivity of 66% and a specificity of 86.8%. For polyps the cut off was 0.41, the sensitivity was 41% and the specificity 77.4%. Conclusion -A higher concentration of hDNA had been found in colorectal cancer patients The quantification of hDNA from the stools can be a trial method for the diagnosis of colorectal cancer.

Fecal Genetic Mutations and Human DNA in Colorectal Cancer and Polyps Patients

“…In cancer patients due to exfoliation of tumor cells average human DNA content (15.05±30.7 ng/ul) was approximately 33 times higher when compared to the control group. Similar results have already been published by our group (Teixeira et al, 2015). Among patients with polyps, no differences were observed when compared to the control group.…”

Eosinophils in the gastrointestinal tract and their role in the pathogenesis of major colorectal disorders

“…One interesting link here is provided by recently published results implicating inflammation-induced ETosis in extracellular matrix remodelling and awakening of dormant cancer cells[237]. As tumour-associated inflammation is a characteristic feature of CRC, and significantly increased extracellular trap formation in these tumours is now proven[238,239], it looks probable that previous reports of CRC-associated increase in the amount of DNA in stool[240,241] or on the surface of colorectal mucosa[190,242] at least partially reflected abundant ETosis occurring within mucus layers contacting tumour surface. In essence, cellular presence in the mucus overlaying colorectal tumour surface is quite similar to that depicted by Figure 3, the abundance of exfoliated malignant cells being the only major difference[190].…”

Characteristics of Differently Located Colorectal Cancers Support Proximal and Distal Classification: A Population-Based Study of 57,847 Patients

“…In other studies, risk factors including black race and a history of cancer or diabetes were closely related to RCC, while factors including white race, smoking, and high alcohol intake were closely associated with both LCC and ReC[15–19]. Recent studies indicated that LCC had higher concentrations of human DNA in the stools than RCC[20], while RCC showed significantly higher mRNA expression levels of glycolysis genes than LCC[21]. Differences were also observed between proximal and distal colon cancers in common alterations of mucosal gene-expression profiles in CRC-associated microbiota [22].…”