Human Dentin as Novel Biomaterial for Bone Regeneration

Murata, Masaru; Akazawa, Toshiyuki; Mitsugi, Masaharu; Um, In-Woong; Kim, Kyung-Wook; Kim, Young-Kyun

doi:10.5772/25071

Cited by 48 publications

(58 citation statements)

References 29 publications

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“…Regarding organics of this demineralized dentin matrix (DDM), there are type I collagen, and various growth factors including BMPs, which induce bone and cartilage, and non-collagenous proteins such as osteocalcin and osteonectin, which have been implicated in calcification. Dentin-specific proteins including phosphoprotein, (phosphophoryn) and dentin sialoprotein have also been identified in dentin matrix 25) . It is well known that appropriate demineralization process does not denature the type I collagens, growth factors, and non-collagenous proteins so that the DDM can maintain osteoinductive-healing capacities [5][6][7]18,25) .…”

Section: Characterization Of Autotooth Bone Graft Materialsmentioning

confidence: 99%

Tooth Bank System for Bone Regeneration - Safety Report -

Kim¹,

Um²,

Murata³

2014

J. Hard Tissue Biology.

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The use of auto-tooth bone grafts fabricated from patients' own extracted teeth has become possible due to the development of tooth banking procedures. The Korea Tooth Bank (KTB), established in Seoul in 2009, is one such tooth-banking facility that can procure and store teeth, and then process them into bone graft substitutes. Another is the Hospital Tooth Bank (HTB) at Seoul National University Bundang Hospital (SNUBH), established in 2010 for performing storage and grafting of auto-tooth bone grafts based on experimental and clinical research. Extracted teeth are sent to the above-mentioned facility units and then delivered back to the patients for clinical use, and thus the safety of the auto-tooth bone graft materials as well as the clinical effectiveness must be guaranteed through proper quality assurance (QA) procedures. For the purpose of this investigation, we analyzed written documents for QA at KTB, and we performed histopathologic and microbiologic examinations against the banked tooth materials at the HTB. The results suggest that the tooth banking systems at both KTB and HTB sufficiently ensure patient safety.

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Section: Characterization Of Autotooth Bone Graft Materialsmentioning

confidence: 99%

Tooth Bank System for Bone Regeneration - Safety Report -

Kim¹,

Um²,

Murata³

2014

J. Hard Tissue Biology.

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“…In other words, DDM has inorganic components, which is the most smilar to autogenous bone, and includes organic components engaged in osteoinduction. DDM has microporous structures named dentinal tubules that could be loaded with bone growth factors such as BMP 15,[17][18][19][20] . In other words, it is con-sidered DDM can play the role of excellent bone graft material and also of carrier of growth factors.…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of Autogenous Tooth Bone Graft Combined with Growth Factor: Prospective Cohort Study

Ahn¹,

Kim²,

Yun³

et al. 2013

Journal of Korean Dental Science

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“…In the bone formation operation, deficient parts of the skeleton for patients have been reconstructed or regenerated by implanting fresh and autogenous bone as reasonable standard materials. To get beyond the need for harvest of grafts, a lot of studies for bone substitute or bone production via bio-engineering have been carried out 1,3) . Generally, unnecessary human teeth (extracted human teeth) are discarded as infective medical wastes.…”

Section: Introductionmentioning

confidence: 99%

“…So we have been investigating osteoinduction and bioabsorption characteristics of human dentin to propose a medical recycle technique of human teeth for bone-regeneration. In 2006, we developed the newly cooling-pulverizing apparatus for teeth that was equipped by a ZrO 2 rotational blade and a ZrO 2 vessel 3,5) .…”

Section: Introductionmentioning

confidence: 99%

“…Generally, unnecessary human teeth (extracted human teeth) are discarded as infective medical wastes. Since demineralized dentin matrix (DDM) originated from human teeth is an osteoinductive and acid-insoluble collagen-matrix, the DDM granules treated by strong acids can be recycled as carriers containing bone growth factors for their own bone-regenerative therapy 3,4) . So we have been investigating osteoinduction and bioabsorption characteristics of human dentin to propose a medical recycle technique of human teeth for bone-regeneration.…”

Section: Introductionmentioning

confidence: 99%

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Bioactive Surface Structure and Bio-Absorption of Human Dentin Granules Designed by the Supersonic Demineralization and Biomimetic Coating Technique

Akazawa

Murata

Minamida

et al. 2012

J. Hard Tissue Biology.

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Demineralized dentin matrix (DDM) granules with excellent biocompatibility were designed using extracted human teeth by the cooling-pulverizing and supersonic demineralizing technique. Extracted teeth were pulverized together with saline ice at 12,000 rpm of a ZrO2 blade for 30 s in a ZrO2 vessel. The pulverized granules having 0.5-2.0 mm in granular size were dissolved by the supersonic treatment at 120 W and 38 kHz or 600 W and 28-100 kHz in 2.0 % HNO3 solutions to obtain partially or completely DDM granules. Dissolution efficiencies of the pulverized granules increased with increasing the supersonic time. At 600 W, dissolution efficiencies for DDM treated at 28 kHz were higher than those at other frequencies. The Ca/P molar ratios of the granules were 1.60-1.66, suggesting that partial DDM granules were composites of Ca 2+ -deficient hydroxyapatite (HAp) and collagen. At 120 W, as supersonic time increased, asperity on the surfaces of granules became outstanding due to elution of mineral components. At 45 min, DDM granules were completely demineralized and the weight decreased to about one-fifth. Hard asperity on surfaces and micro-crack were observed. Partially or completely DDM granules were implanted into the subcutaneous tissues of the back region of rats. At 4 weeks, completely DDM granules had a little smaller and more irregular shape than partially DDM ones. Infection and exclusion of DDM granules were not recognized, but inflammatory cell invasion was seen through bio-absorption of DDM due to the enzyme-digestion. To improve surface activity of DDM, DDM granules were soaked at 309.5 K and pH 7.40 in a simulated body fluid. At 48 h, HAp nano-crystals were almost homogeneously coated on the surfaces of completely DDM granules, while at 144 h, porous bone-like apatites were found. The HAp nano-crystals coating for DDM granules would form bioactive surface with specific pore structure and chemical nature.

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Human Dentin as Novel Biomaterial for Bone Regeneration

Cited by 48 publications

References 29 publications

Tooth Bank System for Bone Regeneration - Safety Report -

Tooth Bank System for Bone Regeneration - Safety Report -

Effectiveness of Autogenous Tooth Bone Graft Combined with Growth Factor: Prospective Cohort Study

Bioactive Surface Structure and Bio-Absorption of Human Dentin Granules Designed by the Supersonic Demineralization and Biomimetic Coating Technique

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