Human Dental Pulp Stem Cells and Gingival Mesenchymal Stem Cells Display Action Potential Capacity In Vitro after Neuronogenic Differentiation

Liu, Dong; Zou, Xiaoying; El-Ayachi, Ikbale; Romero, Luis O.; Yu, Zongdong; Iglesias-Linares, Alejandro; Cordero-Morales, Julio F.; Huang, George T.‐J.

doi:10.1007/s12015-018-9854-5

Cited by 73 publications

(70 citation statements)

References 43 publications

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“…Interestingly, the gene pro ling of differentiated cells was correlated with the recent study, which established neural progenitor cells derived hSCAPs in neurospheres 3D model [50]. We have found that the hSCAPs treated with 10 µM resveratrol for 12 hours created the optimal condition for enhancing the neural progenitor gene, and that the synergistic pre-treatment with resveratrol, and within the optimal condition, triggered differentiation into neural progenitor-like cells with speci c expressions of NES, SOX1 and showed a higher percentage of neuronal differentiation than d-hSCAPs by 4 times.…”

Section: Discussionsupporting

confidence: 58%

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Potential of resveratrol in enrichment of neural progenitor-like cells induction of human stem cells from apical papilla

Songsaad

Gonmanee

Ruangsawasdi

et al. 2020

Preprint

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Introduction: Stem cell transplantation of exogenous neural progenitor cells (NPCs) derived from mesenchymal stem cells (MSCs) has emerged as a promising approach of neurodegenerative disease. Human stem cells from apical papilla (hSCAPs) are derived from migratory neural crest stem cells, and exhibit a potential of neuronal differentiation. However, their neuronal differentiation is low and unpredictable. Resveratrol has been described as a sirtuin 1 (SIRT1) activator which plays an important role in enhancing neuronal differentiation. In this study, we investigate the potential of resveratrol as an enhancer on neuronal differentiation through NPCs induction of hSCAPs.Methods: Stem cells were isolated from human apical papilla, and characterized as MSCs. The cellular toxicity of resveratrol treatment to the characterized hSCAPs was investigated by MTT assay. The non-cellular toxicity concentrations of resveratrol were assessed with various pre-treatment times to select the optimal condition that highly expressed the neural progenitor gene, NES. Consequently, the optimal condition of resveratrol pre-treatment was synergistically performed with a neuronal induction medium to trigger neuronal differentiation. The differentiated cells were visualized, the genes profiling was quantified, and the percentage of neuronal differentiation was calculated. Moreover, the intracellular calcium oscillation was demonstrated. Results: The cellular toxicity of resveratrol was not observed for up to 50 µM for 12 hours. Interestingly, hSCAPs pre-treated with 10 µM resveratrol for 12 hours (RSV-hSCAPs) significantly expressed NES, which is determined as the optimal condition. Under neuronal induction, both of hSCAPs and RSV-hSCAPs were differentiated (d-hSCAPs, and RSV-d-hSCAPs) as they exhibited neuronal-like appearances with Nissl substance staining. The highest expression of NES, and SOX1 was observed in RSV-d-hSCAPs. Additionally, the percentage of neuronal differentiation of RSV-d-hSCAPs was significantly higher than d-hSCAPs for 4 times. Importantly, the neuronal-like cells exhibited slightly increasing pattern of calcium intensity.Conclusion: This study demonstrated that pre-treatment of resveratrol strongly induces neural progenitor marker gene expression which synergistically enhances neural progenitor-like cells’ induction with neuronal induction medium.

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Section: Discussionsupporting

confidence: 58%

“…However, recent study demonstrated that the neural progenitor cells derived from hDPSCs were insu ciency expressed of PAX6. Taken together, highly expressed of NES, SOX1, and weakly expressed of PAX6 pro ling are used as an early stage marker of neuronal differentiation (50).…”

Section: Discussionmentioning

confidence: 99%

Potential of resveratrol in enrichment of neural progenitor-like cells induction of human stem cells from apical papilla

Songsaad

Gonmanee

Ruangsawasdi

et al. 2020

Preprint

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“…for a longer period, as has been described by Gervois et al 15 Other authors have encountered similar problems, when only a small fraction of cells were able to trigger action potentials using the same protocol. 41 It is possible that the heterogeneity present in hDPCs-NCSCs limits a full neurogenic potential. 35 However, the observed hDPC-BMP4 NCSC phenotype, as well as the improved changes observed during their neural differentiation in vitro, support the important role of BMP4 in sensory neuron and cranio-facial neural crest induction.…”

Section: Discussionmentioning

confidence: 99%

Establishment and neural differentiation of neural crest-derived stem cells from human dental pulp in serum-free conditions

Solis-Castro

Boissonade

Rivolta

2020

Stem Cells Translational Medicine

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The potential of obtaining cell cultures with neural crest resemblance (neural crestderived stem cells [NCSCs]) from dental-related tissues, including human dental pulp cells (hDPCs), has been discussed in the literature. However, most reports include the use of serum-rich conditions and do not describe the potential for neural differentiation, slowing translation to the clinic. Therefore, we aimed to culture and characterize NCSCs from the human dental pulp in vitro and evaluate their abil

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“…As shown in Fig. 4A and B , mRNA and protein of Nestin and NFM which are intermediate filament proteins expressed in nerve cells [ 26 ], were clearly increased in 5-aza-CN-treated NIH-3T3 fibroblasts. Fig.…”

Section: Resultsmentioning

confidence: 99%

Direct reprogramming of fibroblasts into diverse lineage cells by DNA demethylation followed by differentiating cultures

Yang

Moon

et al. 2020

Korean J Physiol Pharmacol

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Direct reprogramming, also known as a trans-differentiation, is a technique to allow mature cells to be converted into other types of cells without inducing a pluripotent stage. It has been suggested as a major strategy to acquire the desired type of cells in cell-based therapies to repair damaged tissues. Studies related to switching the fate of cells through epigenetic modification have been progressing and they can bypass safety issues raised by the virus-based transfection methods. In this study, a protocol was established to directly convert fully differentiated fibroblasts into diverse mesenchymal-lineage cells, such as osteoblasts, adipocytes, chondrocytes, and ectodermal cells, including neurons, by means of DNA demethylation, immediately followed by culturing in various differentiating media. First, 24 h exposure of 5-azacytidine (5-aza-CN), a well-characterized DNA methyl transferase inhibitor, to NIH-3T3 murine fibroblast cells induced the expression of stem-cell markers, that is, increasing cell plasticity. Next, 5-aza-CN treated fibroblasts were cultured in osteogenic, adipogenic, chondrogenic, and neurogenic media with or without bone morphogenetic protein 2 for a designated period. Differentiation of each desired type of cell was verified by quantitative reverse transcriptase-polymerase chain reaction/western blot assays for appropriate marker expression and by various staining methods, such as alkaline phosphatase/alizarin red S/oil red O/alcian blue. These proposed procedures allowed easier acquisition of the desired cells without any transgenic modification, using direct reprogramming technology, and thus may help make it more available in the clinical fields of regenerative medicine.

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Human Dental Pulp Stem Cells and Gingival Mesenchymal Stem Cells Display Action Potential Capacity In Vitro after Neuronogenic Differentiation

Cited by 73 publications

References 43 publications

Potential of resveratrol in enrichment of neural progenitor-like cells induction of human stem cells from apical papilla

Potential of resveratrol in enrichment of neural progenitor-like cells induction of human stem cells from apical papilla

Establishment and neural differentiation of neural crest-derived stem cells from human dental pulp in serum-free conditions

Direct reprogramming of fibroblasts into diverse lineage cells by DNA demethylation followed by differentiating cultures

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