Human Deciduous Teeth Dental Pulp Cells With Basic Fibroblast Growth Factor Enhance Wound Healing of Skin Defect

Abstract

In this research, we examined the effect on wound healing applying basic fibroblast growth factor (b-FGF) that is approved for clinical use to enhance wound healing and human deciduous teeth dental pulp cells (hDPCs) in clinics, but that have been attracting attention as a novel stem cell source in recent years. Human deciduous teeth were harvested from healthy volunteers, and hDPCs were isolated. We used a nude mouse full-thickness skin defect model and evaluated wound healing by macroscopic view and histologic and histomorphometric analysis. The mice were randomly divided into 4 groups: phosphate-buffered saline-treated group (control group), b-FGF-treated group (b-FGF group), hDPC-treated group (hDPC group), and hDPC and b-FGF-treated group (hDPC/b-FGF group). Basic fibroblast growth factor and hDPC groups accelerated wound healing compared with the control group. There was no statistically significant difference in wound healing observed between the hDPC and b-FGF groups. The hDPC/b-FGF group demonstrated accelerated wound healing compared with other groups. At day 14, PKH26-positive cells were surrounded by human type I collagen in hDPC and hDPC/b-FGF groups in immunohistologic evaluation. Significantly increased collagen fibril areas in wound tissues were observed in b-FGF, hDPC, and hDPC/b-FGF groups as compared with the control group at days 7 and 14. Our results showed that the hDPC/b-FGF group significantly promotes wound healing compared with other groups. This study implies that deciduous teeth that are currently considered as medical spare parts might offer a unique stem cell resource for potential of new cell therapies for wound healing in combination with b-FGF.

Can SHED or DPSCs be used to repair/regenerate non-dental tissues? A systematic review of in vivo studies

“…22 Angiogenesis was found to be improved when endothelial progenitor cells from swine deciduous and permanent teeth were used to treat ischemia in a hind limb ischemia mouse model, as the cells helped to increase the blood supply and contributed to the formation of a new capillary network. 23 Lastly, SHED were reported 24 to help accelerate wound healing when associated with fibroblast growth factor (b-FGF).…”

“…Hence, the use of endothelial progenitor cells derived from dental pulp might represent an alternative approach for the transplantation of autologous endothelial progenitor cells in which cells are obtained by an invasive and potentially painful biopsy procedure. 30 Lastly, Nishino Y et al, 24 showed that the use of SHED, in association with basic fibroblasowth factor (b-FGF), in a nude mouse full-thickness skin defect model significantly accelerated wound healing compared with other groups. This work highlights SHED as a promising stem cell population for future wound healing therapies as well.…”

Effect of Bone Marrow Derived Mesenchymal Stem Cells on Healing of Induced Full-Thickness Skin Wounds in Albino Rat

“…PKH26 was chosen to label MSCs according to previous study (17). The choice of PKH26 in this study was attributed to its reported advantages as PKH26 is rapid, simple labeling procedure, achieves bright staining without altering protein and cell function and is physiologically stable for up to 4 months in vivo (18)(19)(20).…”

Human Immature Dental Pulp Stem Cells (hIDPSCs), Their Application to Cell Therapy and Bioengineering: An Analysis by Systematic Revision of the Last Decade of Literature

“…The injection of SHED cells improved the skin damage which demonstrated their potential in skin regeneration. When SHED cells associated in vitro with human mesenchymal stromal cells (hMSCs) were transplanted into a wound in a mouse model, the synthesis of collagen was observed resulting in a similar effect on skin regeneration (Nishino et al, ). These same authors also reported the effects of the interaction between the human dental pulp stem cells from deciduous teeth (hDPSCs) and the basic fibroblast growth factor (bFGF) on skin regeneration suggesting that those cells could be used for treatment of epithelial ulceration.…”