Human Cystic Echinococcosis: Old Problems and New Perspectives

Siracusano, Alessandra; Teggi, Antonella; Ortona, Elena

doi:10.1155/2009/474368

Cited by 86 publications

(76 citation statements)

References 59 publications

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“…Both native and recombinant antigens demonstrated similar sensitivities and specificities. However, the latter would be more useful for patient diagnosis because it can be produced as a standardized and reproducible diagnostic reagent (31).…”

Section: Discussionmentioning

confidence: 99%

IgG4 Detection of Echinococcus granulosus Paramyosin Is a Useful Diagnostic Test for Human Hydatidosis

Moghadam

Ghaffarifar

Khalilpour

et al. 2013

Clin Vaccine Immunol

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Hydatidosis is a public health problem in many parts of the world, and improvement in diagnosis of the disease is still being pursued. Protoscoleces of Echinococcus granulosus were isolated from hydatid cysts collected from naturally infected sheep slaughtered in abattoirs in Iran. Sonicated extract of protoscolex was subjected to two-dimensional gel electrophoresis and Western blot analysis. Primary antibodies were from serum samples from 130 hydatidosis patients, 38 individuals infected with other parasitic infections, and 30 healthy people, whereas peroxidase (HRP)-conjugated anti-human IgG and IgG4 were used as secondary antibodies. The recombinant form of the identified protein was produced and tested for its sensitivity and specificity for the detection of human hydatidosis. An antigenic band of ϳ60 kDa was found to be sensitive (82%) and specific (100%) for the detection of hydatidosis when probed with anti-human IgG4-HRP, while the sensitivity and specificity were 33 and 100%, respectively, with anti-human IgG-HRP. By mass spectrometry, the band was identified as protoscolex tegument paramyosin. The sensitivity and specificity of full-length paramyosin-recombinant protein in IgG4 blots were found to be 86 and 98%, respectively. In conclusion, IgG4 detection of Echinococcus granulosus paramyosin was found to be useful for the diagnosis of human hydatidosis.

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Section: Discussionmentioning

confidence: 99%

IgG4 Detection of Echinococcus granulosus Paramyosin Is a Useful Diagnostic Test for Human Hydatidosis

Moghadam

Ghaffarifar

Khalilpour

et al. 2013

Clin Vaccine Immunol

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“…1 In the presence of host immune regulation, an E. granulosus infection usually becomes chronic. Echinococcosis granulosus can trigger a series of hypersensitive reactions, including urticaria, COMPARISONS OF SERUM TOTAL IgE, IgG, AND IgG1 LEVELS transient chills or fever, fatal bronchospasm, angioneurotic edema, and anaphylactic shock.…”

Section: Discussionmentioning

confidence: 99%

“…1 Anaphylactic shock that can result from echinococcosis cyst rupture either during surgery or caused by trauma or spontaneous cyst rupture is a severe complication with an incidence of about 4.6% of CE cases. Echinococcosis-induced anaphylactic shock usually develops rapidly and inappropriate treatment may lead to detrimental consequences and even death.…”

Section: Introductionmentioning

confidence: 99%

Comparisons of Serum Total IgE, IgG, and IgG1 Levels in Patients with and without Echinococcosis-Induced Anaphylactic Shock

Li¹,

Zheng²,

Gu³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. We investigated serum total immunoglobulin E (IgE), IgG, and IgG1 levels in patients with and without echinococcosis-induced anaphylactic shock. This was a case-control study of 11 patients with echinococcosis-induced anaphylactic shock and 22 echinococcosis patients with cyst rupture but without anaphylactic shock. Blood was collected before surgery (T0), at the time of cyst rupture (T1), and shock (Tx), 1 h (T2), 1 day (T3), and 1 week (T4) after cyst rupture. Serum IgE, IgG, and IgG1 were determined by enzyme-linked immunosorbent assay. Serum IgE, IgG, and IgG1 levels were significantly higher in patients who developed anaphylactic shock at all time points. Increased presurgical IgG and IgG1 levels were identified to be a significant risk factors for developing anaphylactic shock. The results showed that a serum IgG concentration of 312.25 μg/mL could be used as a cut-off point to predict whether an echinococcosis patient would develop anaphylactic shock.

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“…Both albendazole (10-15 mg/kg) and mebendazole (40-50 mg/kg) are more effective in cycles of continuous treatment, without intervals. Lung cysts and especially cysts of young people are more responsive to carbamate therapy [4].…”

Section: Open    Accessmentioning

confidence: 99%

Hydatid Disease with Multiple Organ Involvement: A Case Report

Kojundžić¹,

Dolić²,

Buča³

et al. 2010

Macedonian Journal of Medical Sciences

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We present a case of 34 years old woman with hydatid disease of the lungs, brain and heart but without liver involvement. The disease started in 2006. as pulmonary hydatidosis of the right posterior inferior lobe. In July 2008, brain surgery was performed due to the hydatid disease located on the right temporoparietal region based on computed tomography (CT) and magnetic resonance imaging (MRI). During the postsurgical recovery she complained of dyspnea. Heart ultrasonography and computed tomography showed hydatid cyst located in the left ventricle. Because of the possibility of the multiple organ involvement, we suggest that postoperative follow-up of patients with previous hydatid disease should include chest radiographs, ultrasonography of the heart and even brain CT and/or MRI as standard methods.

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Human Cystic Echinococcosis: Old Problems and New Perspectives

Cited by 86 publications

References 59 publications

IgG4 Detection of Echinococcus granulosus Paramyosin Is a Useful Diagnostic Test for Human Hydatidosis

IgG4 Detection of Echinococcus granulosus Paramyosin Is a Useful Diagnostic Test for Human Hydatidosis

Comparisons of Serum Total IgE, IgG, and IgG1 Levels in Patients with and without Echinococcosis-Induced Anaphylactic Shock

Hydatid Disease with Multiple Organ Involvement: A Case Report

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