Human cardiosphere-seeded gelatin and collagen scaffolds as cardiogenic engineered bioconstructs

Chimenti, Isotta; Rizzitelli, G.; Gaetani, Roberto; Angelini, Francesco; Ionta, Vittoria; Forte, Elvira; Frati, Giacomo; Schussler, Olivier; Barbetta, Andrea; Messina, Elisa; Dentini, Mariella; Giacomello, Alessandro

doi:10.1016/j.biomaterials.2011.08.049

Cited by 60 publications

(58 citation statements)

References 54 publications

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“…The created scaffolds fulfill both of these criteria: The elastic modulus of 15–132 kPa displayed by the scaffolds under tension fits well into the range measured for cardiac muscle 20–500 kPa,24 and resides within the interval (7.9–1200 kPa) that has been reported in previous cardiac biomaterial studies 25, 26, 27, 28, 29, 30…”

Section: Discussionsupporting

confidence: 84%

Indirect three‐dimensional printing: A method for fabricating polyurethane‐urea based cardiac scaffolds

Hernández‐Córdova

Mathew

Balint

et al. 2016

J. Biomed. Mater. Res.

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Biomaterial scaffolds are a key part of cardiac tissue engineering therapies. The group has recently synthesized a novel polycaprolactone based polyurethane‐urea copolymer that showed improved mechanical properties compared with its previously published counterparts. The aim of this study was to explore whether indirect three‐dimensional (3D) printing could provide a means to fabricate this novel, biodegradable polymer into a scaffold suitable for cardiac tissue engineering. Indirect 3D printing was carried out through printing water dissolvable poly(vinyl alcohol) porogens in three different sizes based on a wood‐stack model, into which a polyurethane‐urea solution was pressure injected. The porogens were removed, leading to soft polyurethane‐urea scaffolds with regular tubular pores. The scaffolds were characterized for their compressive and tensile mechanical behavior; and their degradation was monitored for 12 months under simulated physiological conditions. Their compatibility with cardiac myocytes and performance in novel cardiac engineering‐related techniques, such as aggregate seeding and bi‐directional perfusion, was also assessed. The scaffolds were found to have mechanical properties similar to cardiac tissue, and good biocompatibility with cardiac myocytes. Furthermore, the incorporated cells preserved their phenotype with no signs of de‐differentiation. The constructs worked well in perfusion experiments, showing enhanced seeding efficiency. © 2016 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1912–1921, 2016.

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Section: Discussionsupporting

confidence: 84%

Indirect three‐dimensional printing: A method for fabricating polyurethane‐urea based cardiac scaffolds

Hernández‐Córdova

Mathew

Balint

et al. 2016

J. Biomed. Mater. Res.

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“…An “ideal” biomaterial should have high elasticity, high extensibility, high robustness and low Young’s modulus [160]. Various biopolymers, such as alginate [161], chitosan [162], gelatin [161, 163], collagen [162, 164], silk [165, 166], fibrin [167], poly(glycerol sebacate) (PGS) [168] have been employed on cardiac tissue engineering, also in conjunction with iPS cells [169, 170]. The group of Yamanaka [171] argued for the autologous approach to cardiac tissue engineering.…”

Section: Application Of Ips Cells In Regenerative Medicine and Cardiamentioning

confidence: 99%

The Current Status of iPS Cells in Cardiac Research and Their Potential for Tissue Engineering and Regenerative Medicine

Martins

Vunjak-Novakovic

Reis

2014

Stem Cell Rev and Rep

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The recent availability of human cardiomyocytes derived from induced pluripotent stem (iPS) cells opens new opportunities to build in vitro models of cardiac disease, screening for new drugs, and patient-specific cardiac therapy. Notably, the use of iPS cells enables studies in the wide pool of genotypes and phenotypes. We describe progress in reprogramming of induced pluripotent stem (iPS) cells towards the cardiac lineage/differentiation. The focus is on challenges of cardiac disease modeling using iPS cells and their potential to produce safe, effective and affordable therapies/applications with the emphasis of cardiac tissue engineering. We also discuss implications of human iPS cells to biological research and some of the future needs.

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“…Furthermore, limited engraftment and proliferation and differentiation potential of the transplanted cells, together with the unsuitable ischemic microenvironment and the progressive myocardial maladaptive remodelling process, hamper the therapeutic outcome [13, 14]. Therefore, increasing the engraftment and regenerative potential of CPCs, as well as their antiremodelling capacities, for example, by means of tissue engineering approaches [15, 16] or pharmacological treatments [14, 17], would be beneficial.…”

Section: Introductionmentioning

confidence: 99%

Normal versus Pathological Cardiac Fibroblast-Derived Extracellular Matrix Differentially Modulates Cardiosphere-Derived Cell Paracrine Properties and Commitment

Pagano

Angelini

Castaldo

et al. 2017

Stem Cells International

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Human resident cardiac progenitor cells (CPCs) isolated as cardiosphere-derived cells (CDCs) are under clinical evaluation as a therapeutic product for cardiac regenerative medicine. Unfortunately, limited engraftment and differentiation potential of transplanted cells significantly hamper therapeutic success. Moreover, maladaptive remodelling of the extracellular matrix (ECM) during heart failure progression provides impaired biological and mechanical signals to cardiac cells, including CPCs. In this study, we aimed at investigating the differential effect on the phenotype of human CDCs of cardiac fibroblast-derived ECM substrates from healthy or diseased hearts, named, respectively, normal or pathological cardiogel (CG-N/P). After 7 days of culture, results show increased levels of cardiogenic gene expression (NKX2.5, CX43) on both decellularized cardiogels compared to control, while the proportion and staining patterns of GATA4, OCT4, NKX2.5, ACTA1, VIM, and CD90-positive CPCs were not affected, as assessed by immunofluorescence microscopy and flow cytometry analyses. Nonetheless, CDCs cultured on CG-N secreted significantly higher levels of osteopontin, FGF6, FGF7, NT-3, IGFBP4, and TIMP-2 compared to those cultured on CG-P, suggesting overall a reduced trophic and antiremodelling paracrine profile of CDCs when in contact with ECM from pathological cardiac fibroblasts. These results provide novel insights into the bidirectional interplay between cardiac ECM and CPCs, potentially affecting CPC biology and regenerative potential.

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Human cardiosphere-seeded gelatin and collagen scaffolds as cardiogenic engineered bioconstructs

Cited by 60 publications

References 54 publications

Indirect three‐dimensional printing: A method for fabricating polyurethane‐urea based cardiac scaffolds

Indirect three‐dimensional printing: A method for fabricating polyurethane‐urea based cardiac scaffolds

The Current Status of iPS Cells in Cardiac Research and Their Potential for Tissue Engineering and Regenerative Medicine

Normal versus Pathological Cardiac Fibroblast-Derived Extracellular Matrix Differentially Modulates Cardiosphere-Derived Cell Paracrine Properties and Commitment

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