Human bronchoalveolar lavage fluid: Two-dimensional gel electrophoresis, amino acid microsequencing and identification of major proteins

Wattiez, Ruddy; Hermans, Cédric; Bernard, Alfred; Lesur, Oliver; Falmagne, Paul

doi:10.1002/(sici)1522-2683(19990601)20:7<1634::aid-elps1634>3.0.co;2-j

Cited by 99 publications

(83 citation statements)

References 43 publications

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“…There have been continuous efforts on the development of reference 2-D maps for human BALF proteins [399][400][401][402]. The current master gel of BALF proteins comprises more than 1200 spots visualized by silver staining [402].…”

Section: Analysis and Characterization Of Balf Proteomementioning

confidence: 99%

“…Gel-solin secretion was found increased three-fold in the airway surface liquid of epithelia treated with IL-4, suggesting that gelsolin might improve the fluidity of airway surface liquid in asthma by breaking down filamentous actin that may be released in large amounts by dying cells during inflammation [417,404]. In addition, a number of 2-DE studies were conducted to monitor the proteomic changes in BALF of patients suffering from lupus erythematosis, Wegener's granulomatosis, lipoid pneumonia, chronic eosinophilic pneumonia, asbestosis, bacterial pneumonia, hypersensitivity pneumonitis, malignancies, immunosuppression, and smoking [399][400][401][402]407,[418][419][420][421].…”

Section: Application To Human Diseases Detectionmentioning

confidence: 99%

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Human body fluid proteome analysis

2006

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The focus of this article is to review the recent advances in proteome analysis of human body fluids, including plasma/serum, urine, cerebrospinal fluid, saliva, bronchoalveolar lavage fluid, synovial fluid, nipple aspirate fluid, tear fluid, and amniotic fluid, as well as its applications to human disease biomarker discovery. We aim to summarize the proteomics technologies currently used for global identification and quantification of body fluid proteins, and elaborate the putative biomarkers discovered for a variety of human diseases through human body fluid proteome (HBFP) analysis. Some critical concerns and perspectives in this emerging field are also discussed. With the advances made in proteomics technologies, the impact of HBFP analysis in the search for clinically relevant disease biomarkers would be realized in the future.

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Section: Analysis and Characterization Of Balf Proteomementioning

confidence: 99%

Section: Application To Human Diseases Detectionmentioning

confidence: 99%

Human body fluid proteome analysis

2006

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“…In the past decades, over 100 human proteins or protein isoforms have been identified in bronchoalveolar lavage fluid from patients with various lung diseases (Wattiez et al, 1999;Lenz et al, 1993;Sadaghdar et al, 1992;Sabounchi-Schütt et al, 2001;Vesterberg et al, 2001). The major challenge today is to identify the lead proteins and validate the potential biomarkers (Turtoi et al, 2011a).…”

Section: Current Advances In Proteomic Analysis Of Bronchoalveolar Lamentioning

confidence: 99%

From Biomarker Discovery to Clinical Evaluation for Early Diagnosis of Lung Surgery-Induced Injury

Tsai¹,

Chen²,

Chang³

et al. 2012

Proteomics - Human Diseases and Protein Functions

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“…Spots on the PVDF membrane were excised and the protein N-terminal amino acid sequences were currently determined at the picomol level by automated Edman degradation. For internal sequencing, samples were digested in the presence of trypsin, and peptides were purified by capillary reverse phase HPLC (39,43).…”

Section: -Dementioning

confidence: 99%

Maternal Tobacco Smoking and Lung Epithelium-Specific Proteins in Amniotic Fluid

et al. 2001

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The bronchiolar 16 kD Clara cell secretory protein (CC16) and the alveolar surfactant-associated protein A (SP-A) are secreted in the amniotic fluid (AF), where they reflect the growth and the maturity of the fetal lung. To evaluate the possible effects of in utero tobacco smoke exposure upon infant bronchoalveolar epithelium function and maturity, CC16 and SP-A levels were determined in AF obtained at term (36 -41 wk) from 28 nonsmoking, 18 smoke-exposed, and 28 smoking mothers with uncomplicated pregnancies. Tobacco smoke exposure was assessed by questionnaire and the assay in AF and maternal urine of cotinine, a stable nicotine metabolite. The specificity of the changes of CC16 and SP-A concentrations in AF was assessed by comparison with nonpulmonary proteins of high-(albumin and transferrin) or low-molecular weight (␤ 2 -microglobulin, retinol binding protein, cystatin-C). Pulmonary and nonpulmonary AF proteins were also compared by two-dimensional gel electrophoresis between smoking and nonsmoking mothers. The levels of CC16 and SP-A as well as low-and high-molecularweight proteins were not significantly different between the three smoking categories. The protein pattern of AF, established by two-dimensional gel electrophoresis, did not reveal any quantitative or qualitative difference between nonsmoking (n ϭ 10), smoke-exposed (n ϭ 5), and smoking mothers (n ϭ 5). By multiple regression analysis of possible determinants, tobacco smoke did not emerge as a significant predictor of CC16 and SP-A concentrations in AF. SP-A level was dependent only on gestational age at birth (r 2 ϭ 0.1, p ϭ 0.001), whereas CC16 correlated only with the levels of low-molecular weight proteins (r 2 ϭ 0.2, p ϭ 0.0001). The latter correlation suggests that CC16 enters AF not only as a result of its secretion at the surface of the respiratory tract but also partly following its elimination by the fetal kidney. This study suggests that maternal smoking during pregnancy is not associated with alterations of the secretory functions of the epithelium of the distal airways and the alveoli at term. Maternal smoking during pregnancy is associated with an increased incidence of respiratory illnesses for children during infancy and later in childhood (1-3). Impaired airway function has also been described in both term and preterm infants of smoking mothers, suggesting that maternal smoking probably alters airway and alveolar growth during fetal life (4 -7). No causal mechanisms for these clinical and functional effects have so far been clearly delineated.The pulmonary epithelium is a major target of injury by tobacco smoke toxicants in adults. Some of these toxicants with lipophilic properties are transferred from the mother to the fetus through the placenta (8, 9) and are likely bioactivated in more toxic compounds by fetal pulmonary epithelial cells (10). One may therefore hypothesize that maternal smoking might alter in utero the development and secretory functions of epithelial cells lining the fetal airways and alveoli.Recently, the m...

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Human bronchoalveolar lavage fluid: Two-dimensional gel electrophoresis, amino acid microsequencing and identification of major proteins

Cited by 99 publications

References 43 publications

Human body fluid proteome analysis

Human body fluid proteome analysis

From Biomarker Discovery to Clinical Evaluation for Early Diagnosis of Lung Surgery-Induced Injury

Maternal Tobacco Smoking and Lung Epithelium-Specific Proteins in Amniotic Fluid

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