Human Bocavirus Infection, Canada

Bastien, Nathalie; Brandt, Ken; Dust, Kerry; Ward, Diane McVey; Li, Yan

doi:10.3201/eid1205.051424

Cited by 218 publications

(280 citation statements)

References 16 publications

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“…Although IgG antibody present in a single serum sample does not reflect an acute infection, a high rate of IgG positive sera from healthy children does suggest that HBoV infection is more common in children than genomic detection has shown previously (Allander et al, 2005;Arnold et al, 2006;Bastien et al, 2006;Choi et al, 2006;Foulongne et al, 2006;Lin et al, 2007;Ma et al, 2006;Qu et al, 2007;Sloots et al, 2006;Weissbrich et al, 2006). Infection with B19 occurs in children in a high incidence asymptomatically.…”

Section: Discussionmentioning

confidence: 91%

ELISAs using human bocavirus VP2 virus-like particles for detection of antibodies against HBoV

Lin

Guan

Cheng

et al. 2008

Journal of Virological Methods

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Human bocavirus (HBoV) has been identified worldwide in children with lower respiratory tract infections with an incidence of approximately 2% −11%. The role of HBoV in pathogenesis, however, is largely unknown, and little is known about the epidemiology of the virus. To study the seroepidemiology of HBoV infection, the capsid protein was expressed in insect cells. Expression of the putative major capsid protein VP2 in insect cells led to the formation of virus-like particles exhibiting the typical icosahedral appearance of parvoviruses with a diameter of approximately 20 nm. The expressed particles were used to establish an ELISA method, and serum samples from groups of children of various ages in China were tested for IgG antibodies against HBoV. HBoV antibodies were detected in as high as 36% of healthy children under 9 years. Of children hospitalized with lower respiratory tract infections, 31% were seropositive, and all age groups of these children showed a significantly higher level of HBoV IgG antibody than their healthy counterparts. When divided into age cohorts, results showed that more than 48% of healthy children had seroconverted by age of 4. Thus, HBoV appears to be a common infection in children. The potential pathogenesis of this virus, especially its role in lower respiratory tract infections in children warrants further investigation.

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Section: Discussionmentioning

confidence: 91%

ELISAs using human bocavirus VP2 virus-like particles for detection of antibodies against HBoV

Lin

Guan

Cheng

et al. 2008

Journal of Virological Methods

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“…According to the literature, the prevalence of hBoV infection among hospitalized children with respiratory tract infection ranges between 1.5-18.3% (Bastien et al 2006, Kaplan et al 2006, Allander et al 2007). In our study, we found a prevalence of 13.2%, which is one of the highest reported so far.…”

Section: Discussionmentioning

confidence: 99%

“…Human parvovirus B19, the only other parvovirus that is pathogenic to humans, is also seasonal, with peak occurrences in spring and summer (Heegaard & Brown 2002). Bastien et al (2006) observed no seasonal prevalence, which may be reflected by the low prevalence in their study (Bastien et al 2006). Nonetheless, as highlighted by Schildgen et al (2008), it is very difficult to draw any definitive conclusion on the seasonal distribution of a new viral agent based only on analyses of a single season because there is much variation of the peak respiratory season from year-to-year (Schildgen et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil

Pilger¹,

Cantarelli

Amantéa

et al. 2011

Mem. Inst. Oswaldo Cruz

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The introduction of newer molecular methods has led to the discovery of new respiratory viruses, such as human metapneumovirus (hMPV) and human bocavirus (hBoV), in respiratory tract specimens. We have studied the occurrence of hMPV and hBoV in the Porto Alegre (PA) metropolitan area, one of the southernmost cities of Brazil, evaluating children with suspected lower respiratory tract infection from May 2007-June 2008. A real-time polymerase chain reaction method was used for amplification and detection of hMPV and hBoV and to evaluate coinfections with respiratory syncytial virus (RSV), influenza A and B, parainfluenza 1, 2 and 3, human rhinovirus and human adenovirus. Of the 455 nasopharyngeal aspirates tested, hMPV was detected in 14.5% of samples and hBoV in 13.2%. A unique causative viral agent was identified in 46.2% samples and the coinfection rate was 43.7%. For hBoV, 98.3% of all positive samples were from patients with mixed infections. Similarly, 84.8% of all hMPV-positive results were also observed in mixed infections. Both hBoV and hMPV usually appeared with RSV. In summary, this is the first confirmation that hMPV and hBoV circulate in PA; this provides evidence of frequent involvement of both viruses in children with clinical signs of acute viral respiratory tract infection, although they mainly appeared as coinfection agents

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“…PCR is the most common method used to detect acute infections of HBoV1 (Bastien et al, 2006;Kesebir et al, 2006;Allander et al, 2007;Fry et al, 2007;Schildgen et al, 2008). However, HBoV1 DNA has been shown to remain detectable by PCR in respiratory samples for several months after primary infection (Brieu et al, 2008;Blessing et al, 2009;Lehtoranta et al, 2012;Martin et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Original antigenic sin with human bocaviruses 1–4

Kantola

Hedman

et al. 2015

Journal of General Virology

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Human bocavirus (HBoV) 1 is a widespread parvovirus causing acute respiratory disease in young children. In contrast, HBoV2 occurs in the gastrointestinal tract and is potentially associated with gastroenteritis, whilst HBoV3 and -4 infections are less frequent and have not yet been linked with human disease. Due to HBoV1 DNA persistence in the nasopharynx, serology has been advocated as a better alternative for diagnosing acute infections. In constitutionally healthy children, we previously noted that pre-existing HBoV2 immunity in a subsequent HBoV1 infection typically resulted in low or non-existent HBoV1-specific antibody responses. A phenomenon describing such immunological events among related viruses has been known since the 1950s as 'original antigenic sin' (OAS). The aim of this study was to characterize this putative OAS phenomenon in a more controlled setting. Follow-up sera of 10 rabbit pairs, inoculated twice with HBoV1-4 virus-like particles (VLPs) or control antigens, in various combinations, were analysed with HBoV1-4 IgG enzyme immunoassays with and without depletion of heterotypic HBoV antibodies. There were no significant IgG boosts after the second inoculation in either the heterologously or the homologously HBoV-inoculated rabbits, but a clear increase in cross-reactivity was seen with time. We could, however, distinguish a distinct OAS pattern from plain cross-reactivity: half of the heterologously inoculated rabbits showed IgG patterns representative of the OAS hypothesis, in line with our prior results with naturally infected children. HBoVs are the first parvoviruses to show the possible existence of OAS. Our findings provide new information on HBoV1-4 immunity and emphasize the complexity of human bocavirus diagnosis.

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Human Bocavirus Infection, Canada

Abstract: Human Bocavirus was detected in 18 (1.5%) of 1,209 respiratory specimens collected in 2003 and 2004 in Canada. The main symptoms of affected patients were cough (78%), fever (67%), and sore throat (44%). Nine patients were hospitalized; of these, 8 (89%) were <5 years of age.

Cited by 218 publications

References 16 publications

ELISAs using human bocavirus VP2 virus-like particles for detection of antibodies against HBoV

ELISAs using human bocavirus VP2 virus-like particles for detection of antibodies against HBoV

Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil

Original antigenic sin with human bocaviruses 1–4

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