Human Bocavirus, a Respiratory and Enteric Virus

Vicente, Diego; Cilla, Gustavo; Montes, Milagrosa; Pérez-Yarza, Eduardo González; Pérez-Trallero, Emilio

doi:10.3201/eid1304.061501

Cited by 202 publications

(214 citation statements)

References 7 publications

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“…In this study, a detection rate of 5.8% was observed for HBoV-1 or HBoV-3, which was similar to that reported by other studies conducted in different parts of the world , Vicente et al 2007, including Brazil (Albuquerque et al 2007). Based on the mean occurrence of other gastroenteric viruses in the region, HBoV-1 could be considered the third most common after RVAs and human cali-civiruses (Cardoso et al 2003, Costa et al 2004, Borges et al 2006, Andreasi et al 2008.…”

Section: Discussionsupporting

confidence: 91%

“…HBoV detection in faecal samples from individuals with acute gastroenteritis has gained importance following the positive identification of bocavirus in faecal samples from individuals throughout the world (Albuquerque et al 2007, Lau et al 2007, Monteny et al 2007, Vicente et al 2007, Han et al 2009, Chow et al 2010, Kantola et al 2010, Jin et al 2011. In Brazil, only three studies have been published regarding HBoV detection in children with acute gastroenteritis (Albuquerque et al 2007, Santos et al 2010, Proença-Modena et al 2011 and this is the first study conducted in the Central-West Region of Brazil.…”

Section: Discussionmentioning

confidence: 99%

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Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil

Sousa¹,

Souza²,

Fiaccadori³

et al. 2012

Mem. Inst. Oswaldo Cruz

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To determine the positivity rate of human bocavirus (HBoV) 1 and 3 among children who presented with acute gastroenteritis symptoms during the period of 1994-2004 in the Central-West Region of Brazil, 762 faecal samples were tested using polymerase chain reaction (PCR) for the detection of HBoV DNA. Primers for a segment of the non-structural viral protein 1 (NS1) gene of HBoV-1 and HBoV-3 were used. Twelve HBoV-positive samples were further characterised via genomic sequencing and phylogenetic analysis. Of the samples tested, 5.8% (n = 44) were positive for HBoV-1 or HBoV-3 and co-infection was observed in 14 (31.8%) of the 44 HBoV-positive samples. Nine of the 14 samples were also positive for Rotavirus A and five were positive for Aichi virus. The genomic sequencing of the NS1 partial sequence of 12 HBoV-samples showed that 11 samples were characterised as HBoV-1 and that one was characterised as HBoV-3. The phylogenetic analysis showed that the HBoV-1 samples had a high sequence homology to others previously identified in China, Sweden and Brazil. This is the first study conducted in the Central-West Region of Brazil to detect HBoV-1 and HBoV-3 in faecal samples from children with acute gastroenteritis. Further studies are required to define the role of HBoVs as aetiological agents of gastroenteritis

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil

Sousa¹,

Souza²,

Fiaccadori³

et al. 2012

Mem. Inst. Oswaldo Cruz

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“…Most prevalence studies were done on samples from children with respiratory tract diseases, with rates observed between 1.5-19% (1,(4)(5)(6)(7)(8)(9)(10)(11)(12). Studies made on fecal specimens indicate that HBoV is also prevalent in children with acute gastroenteritis, with 0.8-16% of samples being HBoV + (4,(13)(14)(15). In addition, HBoV infection with complex clinical conditions in immunosuppressed patients has been suggested (16).…”

mentioning

confidence: 99%

Human Bocavirus NP1 Inhibits IFN-β Production by Blocking Association of IFN Regulatory Factor 3 with IFNB Promoter

Zhang

Zheng

Luo

et al. 2012

The Journal of Immunology

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Human bocavirus (HBoV) mainly infects young children. Although many infected children suffer from respiratory or gastroenteric tract diseases, an association between HBoV and these diseases is not definite. Because modulation of type I IFN is crucial for viruses to establish efficient replication, in this study, we tested whether HBoV modulates type I IFN production. We observed that a nearly full-length HBoV clone significantly reduced both Sendai virus (SeV)- and poly(deoxyadenylic-thymidylic) acid-induced IFN-β production. Further study showed that NP1 blocked IFN-β activation in response to SeV, poly(deoxyadenylic-thymidylic) acid, and IFN-β pathway inducers, including retinoic acid-inducible protein I, mitochondrial antiviral signaling protein, inhibitor of κB kinase ε, and TANK-binding kinase 1. In addition, NP1 interfered with IRF-3–responsive PRD(III-I) promoter activated by SeV and a constitutively active mutant of IRF-3 (IRF-3/5D). Although NP1 suppressed the IRF-3 pathway, it did not affect IRF-3 activation processes, including phosphorylation, dimerization, and nuclear translocation. Coimmunoprecipitation assays confirmed the interaction between NP1 and IRF-3. Additional deletion mutagenesis and coimmunoprecipitation assays revealed that NP1 bound to the DNA-binding domain of IRF-3, resulting in the interruption of an association between IRF-3 and IFNB promoter. Altogether, our results indicate that HBoV NP1 blocks IFN production through a unique mechanism. To our knowledge, this is the first study to investigate the modulation of innate immunity by HBoV. Our findings suggest a potential immune-evasion mechanism used by HBoV and provide a basis for better understanding HBoV pathogenesis.

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“…Three other strains of hBoV were discovered in 2010 and are now referred to as hBoV2, hBoV3 and hBoV4 [37][38][39]. HBoV1 causes respiratory diseases and is present everywhere across the globe accounting for about 19 % of all viral infections of the upper and lower respiratory tract in humans [40][41][42]. HBoV1 effectively infects epithelial cells of human airways and induces their cytolysis [43][44][45].…”

Section: Human Bocavirusmentioning

confidence: 99%

Emergence of new infections in the 21st century and identification of pathogens using next generation sequencing

et al. 2017

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Каждое новое инфекционное заболевание может быть серьезным вызовом для современной медицины. Измене-ние окружающей среды, вырубка тропических лесов, таяние льдов в Антарктике, увеличение плотности населения и повсеместное использование антибиотиков -все это факторы, провоцирующие появление новых патогенов. Эпидемии гриппа, вызываемые новыми штаммами вируса; респираторные синдромы, вызываемые новыми коро-навирусами; вспышки инфекций, причиной которых является гемолитическая кишечная палочка; возникновение резистентных к антибиотикам супербактерий -примеры того, какие опасности могут поджидать человечество. В число первоочередных мер, которые необходимо предпринять для противодействия новым патогенам, входит раз-работка способов их быстрой и точной идентификации. В обзоре рассмотрены случаи возникновения в XXI веке новых инфекционных агентов, а также проанализированы возможность и перспективы использования для выявления и идентификации новых патогенов методов высокопроизводительного секвенирования (next generation sequence).Ключевые слова: инфекционные агенты, патогенные организмы, методы выявления новых патогенов, высокопроиз-водительное секвенирование, коронавирус, вирус гриппа, горизонтальный перенос генов, антибиотикорезистентность CENTURY AND IDENTIFICATION OF PATHOGENS USING NEXT GENERATION SEQUENCINGEach new emerging infection may become a big challenge to the medical community. Changing environment, tropical deforestation, melting of the Antarctic ice, growing population density and uncontrolled use of antibiotics provoke emergence and evolution of pathogens. Epidemics caused by new strains of the influenza virus, respiratory syndromes associated with coronaviruses, outbreaks of hemolytic Escherichia coli infections and antibiotic-resistant superbacteria are hazards to humans. Among high-priority measures for pathogen control that are yet to be taken is development of fast and accurate techniques for pathogen identification. Our review looks at the cases of new infections registered in the 21 st century and explores feasibility of next generation sequencing for the detection and identification of new pathogens.

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Human Bocavirus, a Respiratory and Enteric Virus

Cited by 202 publications

References 7 publications

Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil

Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil

Human Bocavirus NP1 Inhibits IFN-β Production by Blocking Association of IFN Regulatory Factor 3 with IFNB Promoter

Emergence of new infections in the 21st century and identification of pathogens using next generation sequencing

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