Human amniotic membrane as a delivery vehicle for stem cell-based therapies

Chen, Ping; Lu, Man; Wang, Tao; Dian, Dongchun; Zhong, Yong; Aleahmad, Mehdi

doi:10.1016/j.lfs.2021.119157

Cited by 14 publications

(14 citation statements)

References 95 publications

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“…An example is decellularized amniotic membrane (AM), which is a promising material in human tissue repair and regeneration due to its biocompatibility, biodegradability, and preservation of abundant bioactive components [ 146 ]. A drug delivery system based on human amniotic extracellular matrix (HAECM) was developed to facilitate endometrial regeneration [ 147 ]. Another example is that well dispersed 17β-estradiol (E 2 )-loaded PLGA microspheres (E 2 -MS) in the scaffolds, realizing the sustained release of E 2 for 21 days [ 148 ].…”

Section: Clinical Application Of Stem Cells and Improvement Strategiesmentioning

confidence: 99%

Research progress of stem cell therapy for endometrial injury

Cen¹,

Zhang²,

Bai³

et al. 2022

Materials Today Bio

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Section: Clinical Application Of Stem Cells and Improvement Strategiesmentioning

confidence: 99%

Research progress of stem cell therapy for endometrial injury

Cen¹,

Zhang²,

Bai³

et al. 2022

Materials Today Bio

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“…The cellular components of AM have active suppression activity on the immune cells' activity through a strong paracrine secretion. This suggests that AM may have an immunomodulatory effect after transplantation, preventing the cellular cargo from being rejected [43]. Due to its success to prevent an allogenic or xenogenic immunologic reaction, AM has gained great interest in transplantation and tissue engineering.…”

Section: Low Immunogenicity and Immunomodulatorymentioning

confidence: 99%

Amniotic Membrane: An Approach to Periodontal Regeneration

Law¹,

Taib²,

Berahim³

2022

Cureus

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Over the years, various materials have been used for scaffold-based periodontal tissue engineering to regenerate lost periodontal tissues. The use of amniotic membrane (AM) as a scaffold for periodontal regeneration has gained great interest among researchers. This narrative review aims to appraise the properties of AM and its potential clinical applications in periodontal regeneration. PubMed, ScienceDirect, Scopus, and Wiley Online Library databases were searched for relevant articles that highlighted the properties and applications of AM in periodontal regeneration. AM has a unique structure and components contributing to its exceptional properties such as anti-inflammatory (presence of anti-inflammatory factors), low immunogenicity (presence of human leukocyte antigen-G), anti-scarring (downregulation of transforming growth factor-β), antimicrobial (expression of antimicrobial factors), promotion of epithelialization (production of growth factors), and reduction of pain (protection of exposed nerve endings). Its use in the treatment of periodontal tissue defect has shown to be effective. AM showed various beneficial properties as an ideal scaffold. Future studies and long-term clinical trials on the efficacy and survival rate of AM are required to completely understand the potential application of AM in periodontal regeneration.

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“…Human amniotic mesenchymal stem cells (hAMSCs), a kind of embryonic derived mesenchymal stem cell, are derived from fetal amniotic membrane tissues. Human amniotic mesenchymal stem cells (hAMSCs) are one of the ideal therapeutic stem cells because of their easy accessibility, low immunogenicity, and less ethical controversy (Chen et al 2021;Li et al 2020), thus have the potential to be widely used in clinical research and Biomedical engineering (Farhadihosseinabadi et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Exosomes derived from human amniotic mesenchymal stem cells promotes angiogenesis in hUVECs by delivering novel miRNA N-194

Song,

Zhang,

Shi

et al. 2024

Preprint

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Background: To investigate the effect and mechanism of exosomes derived from human amniotic mesenchymal stem cells (hAMSC-Exos) promoting angiogenesis. Methods: HAMSC-Exos were isolated using ultracentrifugation and characterized by transmission electron microscopy, NTA, and Western blot. The uptake of hAMSC-Exos by hUVECs was analyzed using PKH-26 labeling, and the effect of hAMSC-Exos on angiogenesis was analyzed in human umbilical vein endothelial cells hUVECs by cell viability assay, Transwell migration assay, Matrigel tube formation assay, and Matrigel plug assays in nude mice. Bioinformatics methods were used to analyze miRNA high-throughput sequencing data of hAMSC-Exos, and RT-qPCR was used to validate the novel miRNAs. HAMSC-Exos with high and low N-194 expression were obtained by transfection, respectively. Target genes were predicted using TargetScan, and the mRNA and protein levels of potential target genes were analyzed by RT-qPCR and Western blot after N-194 mimics transfection. Interaction between miRNAs and target genes was detected using the dual-luciferase reporter assay. Target genes were overexpressed in hUVECs by transfection. The roles of target genes in the influence of N-194 on cell function were determined by analyzing angiogenesis. Results: The extracted hAMSC-Exos showed teato-like under transmission electron microscopy, and the NTA results showed the particle size of 115.6±38.6 nm. The positive expression of CD9, CD63, and CD81 were verified using Western blot. The treatment of hUVECs with hAMSC-Exos significantly increased cell proliferation, migration, and angiogenesis. HAMSC-Exos contained the novel miRNAs N-194, N-314, N-19, N-393, and N-481, and the expression of N-194 was higher. The Exos derived from hAMSCs which were transfected with FAM-N-194 mimics were able to deliver FAM-N-194 mimics to hUVECs. The hAMSC-Exos with high N-194 significantly promoted angiogenesis in hUVECs. N-194 mimics transfection significantly reduced mRNA and protein levels of potential target gene ING5, and N-194 mimics significantly reduced the luciferase activities expressed by wild-type reporter gene vectors for ING5. The ING5 overexpression significantly reduced the angiogenic capacity of hUVECs. ING5 overexpression suppressed the expression of HSP27 and PLCG2. Conclusions: HAMSC-Exos promotes angiogenesis in hUVECs by delivering novel miRNA N-194 which targets ING5.

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Human amniotic membrane as a delivery vehicle for stem cell-based therapies

Cited by 14 publications

References 95 publications

Research progress of stem cell therapy for endometrial injury

Research progress of stem cell therapy for endometrial injury

Amniotic Membrane: An Approach to Periodontal Regeneration

Exosomes derived from human amniotic mesenchymal stem cells promotes angiogenesis in hUVECs by delivering novel miRNA N-194

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