Human Amniotic Membrane and Titanium Dioxide Nanoparticle Derived Gel for Burn Wound Healing in a Rat Model

Islam, Mohammad Rakibul; Karmakar, Polash Chandra; Tusher-Al-Arafat,; Arifuzzaman, Md.; Karim, Nurul Huda Abd; Akhtar, Naznin; Asaduzzaman, S. M.

doi:10.1007/s40883-022-00280-z

Cited by 7 publications

(8 citation statements)

References 39 publications

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“…In this research, AgNPs incorporated HAM gel was assessed and compared preclinically with HAM and nanoparticle separately and with commercially available burn healing cream as a biological dressing for its healing efects. Here, HAM showed healing capabilities on burn wounds which had been used in burns treatment for decades [30,31]. Koob et al demonstrated that HAM was efective for wound healing and contained collagen (type IV, VII, XV, XVI, XVII, and XVIII); glycoproteins such as laminin (α3, β1, β2, β3, c1, and c2 chains), nidogen-1 and nidogen-2, fbronectin, fbulin-2, fbrillin-2; and proteoglycans such as perlecan and agrin play a vital role in wound healing [32].…”

Section: Discussionmentioning

confidence: 99%

Silver Nanoparticle Incorporated Human Amniotic Membrane Gel Accelerates Second‐Degree Burn Wound Healing in Wister Rat

Jhumi

Arafat

Asaduzzaman

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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Burn has terrible consequences for the affected patients, making them vulnerable to wound infections and septicemia, which results in physical and mental disability and death, necessitating superior treatment options. Human amniotic membrane (HAM) has been utilized in burn wounds for decades for its low immunogenicity, angiogenic, anti-inflammatory, and antimicrobial properties and for promoting epithelialization. Silver nanoparticles (AgNPs), on the other hand, have antimicrobial properties and promote fibroblast migration. This study aimed to determine the burn wound healing potential of HAM + AgNPs. The gel was prepared using HAM (1% and 2%), AgNPs, carbopol 934, acrylic acid, glycerine, and triethanolamine, and different physical properties (pH, water absorption, swelling variation, spreadability, etc.) of the gel were determined; nuclear magnetic resonance (NMR) spectroscopy, antibacterial activity, brine shrimp lethality test, and histopathological observation were conducted. In vivo studies with Wistar rats demonstrated better healing capabilities than individual components of the gel. Wound contraction percentage after 20 days was 96.1 ± 0.27% which was highly significant ( p < 0.0001), and the epithelialization period was 23.67 ± 2.05 days ( p < 0.01) for HAM + AgNPs which was preferable to the positive control, AgNPs, HAM, and negative control; also, the histopathologic observation using hematoxylin and eosin, and Masson’s trichrome staining were showed the better healing progress for HAM + AgNPs. Both HAM and AgNPs had antibacterial activities against gram-positive and gram-negative bacteria. These results indicated that the formulated HAM + AgNPs gel had remarkable effectiveness in burn wound healing compared to others. Further studies will be conducted to determine the molecular mechanism behind wound healing.

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Section: Discussionmentioning

confidence: 99%

Silver Nanoparticle Incorporated Human Amniotic Membrane Gel Accelerates Second‐Degree Burn Wound Healing in Wister Rat

Jhumi

Arafat

Asaduzzaman

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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“…For instance, Jhumi et al (2023) have shown that the incorporation of Ag NPs into AM gels accelerates the healing of second-degree burn wounds in terms of expedited re-epithelialization and elevated contraction. It is noteworthy that in this study and many others ( Rahman et al, 2019 ; Rana et al, 2020 ; Islam et al, 2023 ; Jhumi et al, 2023 ), AM tissue has been utilized without decellularization.…”

Section: Biomedical Applications Of Am-derived Hydrogelsmentioning

confidence: 93%

“…Their results indicate that the AM-containing hydrogel facilitates the healing process without a sign of scar formation and promotes re-epithelialization in a quicker time, as compared to the control groups (i.e., 1% silver sulfadiazine and the non-treated group as positive and negative control, respectively). Islam et al (2023) have shown the positive effect of titanium dioxide (TiO 2 ) nanoparticles (NPs) in AM gels containing Carbopol 934 (as a gelling agent) for the treatment of second-degree burn wounds. Their study reveals that treating burn wounds with AM-TiO 2 gels enhances wound closure and re-epithelialization while encouraging vascular formation.…”

Section: Biomedical Applications Of Am-derived Hydrogelsmentioning

confidence: 99%

“…Hence, processing dAM into hydrogels provides the opportunity to fill irregularly shaped cavities. Islam et al (2023) have recently employed a thermosensitive dAM hydrogel to prevent IUA in rat models. It has been shown that the injection of the dAM hydrogel into the uterus cavity can significantly decrease fibrosis formation through re-epithelialization of the damaged endometrium ( Figures 5Ai, ii ), while supporting angiogenesis within the regenerated endometrium ( Figures 5Aiii, iv ).…”

Section: Biomedical Applications Of Am-derived Hydrogelsmentioning

confidence: 99%

“…Therefore, processing of the AM tissue into a hydrogel can afford easier handling by gelation inside any nonuniform defect injuries. To this end, some researchers have developed a gel formula based on the incorporation of AM powder or AM extracts into a hydrogel solution ( Murphy et al, 2017 ; Rahman et al, 2019 ; Islam et al, 2023 ). In other studies, the development of an injectable hydrogel through the enzymatic digestion of decellularized amnion (dAM) powder in a mildly acidic solution has been performed ( Ryzhuk et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

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