Human Amniotic Membrane and Its Anti-cancer Mechanism: a Good Hope for Cancer Therapy

Hossain, Liakat; Siddika, Ayesha; Adnan, Muhammad; Diba, Farzana; Hasan, Zahid; Asaduzzaman, SM

doi:10.1007/s42399-019-00090-5

Cited by 9 publications

(8 citation statements)

References 76 publications

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“…Other authors have also commented that the transparency of amniotic membrane allows for monitoring of tumour recurrence in deeper tissues, and may also provide a superior cosmesis when compared with thicker (for example, buccal) mucous membrane grafts. 125 Although the anti-neoplastic mechanism of amnion is not well understood, 10 it is attributed to the secretion of anti-angiogenic, pro-apoptotic and immune-modulatory factors. 11,126,129 Amnion contains interleukins (IL-2, IL-3 and IL-4) and can express cytotoxic cytokines, these are known to enhance the cytotoxicity of natural killer cells, which are able to attack mitotic cancer cells.…”

Section: Neoplasiamentioning

confidence: 99%

“…1 This is possible due to a myriad of biological properties, including a lack of immunogenicity, thus reducing the risk of inciting an immune response. 8 AMT also preserves and supports stem cells 9 whilst inhibiting neoplastic, 10,11 inflammatory, 12,14 angiogenic and fibroblastic cells. 1,12 When applied in combination these biological properties support and facilitate wound healing.…”

Section: Introductionmentioning

confidence: 98%

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<p>Amniotic Membrane Transplantation in Ophthalmology: An Updated Perspective</p>

Walkden¹

2020

OPTH

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The aim of this paper is to provide a succinct literature review of the different clinical applications for AMT usage in an ophthalmic setting, ranging from commonly used applications to less mainstream approaches. The hope is that this review enables the reader to have a better understanding of the biological properties of amnion as well as the indications and scenarios in which AMT can be used, whilst presenting relevant evidence from within the literature which may be of interest. We also provide an update on the methods of preservation of amniotic membrane and the application methodologies. Methods: Literature search. A PubMed search was performed using the search terms "amniotic membrane transplant", "amnion AND cornea", amnion AND ophthalmology", "amnion AND ocular surface" and "Amnion AND eye". A full review of the literature using the PubMed database was conducted up until 01/05/20. The articles used were written in English, with all articles accessed in full. Both review articles and original articles were used for this review. All full publications related to ophthalmology were considered.

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Section: Neoplasiamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

<p>Amniotic Membrane Transplantation in Ophthalmology: An Updated Perspective</p>

Walkden¹

2020

OPTH

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“… 35 - 37 Furthermore, the evidence has been revealed the antiangiogenic and cell cycle inhibitory properties of amnion. 14 , 38 , 39 Some recent studies have speculated antitumor properties not only for human amniotic stromal cells but also for intact hAM and its conditioned medium. 40 , 41 Accordingly, the present study aims to study the anticancer effects of the human amniotic membrane conditioned medium (hAM-CM) on breast cancer cell lines.…”

Section: Introductionmentioning

confidence: 99%

Tumor Targeting by Conditioned Medium Derived From Human Amniotic Membrane: New Insight in Breast Cancer Therapy

Jafari

Rezaei‐Tavirani

Niknejad

et al. 2021

Technol Cancer Res Treat

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Objectives: Traditional breast cancer treatments have challenges including inefficiency, multidrug resistance, severe side effects, and targeting non-specifically. The development of alternative treatment strategies has attracted a great deal of interest. Using the amniotic membrane has become a promising and convenient new approach for cancer therapy. This study aimed to evaluate the anti-cancer ability of conditioned medium extracted from the human amniotic membrane (hAM-CM) on breast cancer cells. Methods: Conditioned medium was collected after 48 h incubation of hAM in epithelial up manner. MTT, cell cycle, apoptosis, colony formation, and sphere assays were used to determine the impact of hAM-CM on breast cancer cell lines. The effects of hAM-CM on the migration and invasion of breast cancer cells were determined using scratch wound healing and transwell assays, respectively. Results: Based on the results, cell viability was significantly decreased by hAM-CM in a dose-dependent manner. The hAM-CM remarkably induced apoptosis and necrosis of cancer cells. Moreover, cell migration and invasion potential of cancer cells decreased after the hAM-CM treatment. Further, both the number of colonies and their morphologies were affected by the treatment. In the treated group, a significant decrease in the number of colonies along with an obvious change in their morphologies from holoclone shape to a dominant paracolone structure was observed. Conclusion: Our results indicate that the conditioned medium derived from the human amniotic membrane able to inhibit proliferation and metastasis of tumor cells and can be considered a natural and valuable candidate for breast cancer therapy.

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“…It is also well known that AM has anti-inflammatory and antifibrotic effects. 13,14,16,17 AM also shows little immunogenicity. 14,16,18 AM has already been used clinically in other fields, 13 particularly in the field of ophthalmology, such as corneal disease.…”

Section: Introductionmentioning

confidence: 99%

“…The advantage of the AM compared to decellularized bioscaffolds is that it contains stem cells and growth factors, which is expected to have more powerful regenerative effects even for severe VF scar. It is also well known that AM has anti‐inflammatory and antifibrotic effects 13,14,16,17 . AM also shows little immunogenicity 14,16,18 .…”

Section: Introductionmentioning

confidence: 99%

The Effects of Amniotic Membrane Transplantation on Vocal Fold Regeneration

et al. 2021

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Objectives/Hypothesis Vocal fold (VF) scar and sulcus cause severe vocal problems, but optimal methods have not been established. Total replacement of the mucosa is required particularly for cases in which the whole lamina propria is occupied by severe fibrosis and vibratory function is totally lost. The amniotic membrane (AM) has been proven to have regenerative potential, as it contains stem cells and growth factors. The current study investigated the biocompatibility and effects of AM for regeneration of the VF mucosa. Study Design In vitro and in vivo studies. Methods Vocal fold fibroblasts (VFFs) from 13 Sprague–Dawley rats were seeded on AM and subjected to histology and immunohistochemistry, and gene expressions in the VFFs on AM were examined in in vitro study. Twelve New Zealand White rabbits were used in in vivo study. VFs were stripped down and were reconstructed with AM. The regenerative effects were examined 3 months later by histological examination. Results In vitro study indicated VFFs survived on AM and stained positively for Ki67, vimentin, and fibronectin. Gene expressions of Has1, Has2, and Hgf were significantly increased in the VFFs on AM compared with the other groups. The in vivo study indicated AM‐transplanted VFs showed a significantly higher density of hyaluronic acid and lower density of collagen compared with sham VFs. Conclusions The current preliminary study suggests biocompatibility and possible regenerative effects of AM for VFs. Level of Evidence NA Laryngoscope, 132:2017–2025, 2022

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Human Amniotic Membrane and Its Anti-cancer Mechanism: a Good Hope for Cancer Therapy

Cited by 9 publications

References 76 publications

<p>Amniotic Membrane Transplantation in Ophthalmology: An Updated Perspective</p>

<p>Amniotic Membrane Transplantation in Ophthalmology: An Updated Perspective</p>

Tumor Targeting by Conditioned Medium Derived From Human Amniotic Membrane: New Insight in Breast Cancer Therapy

The Effects of Amniotic Membrane Transplantation on Vocal Fold Regeneration

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