Human amniotic glycodelin actively regulates changes in β-catenin immunoreactivity in cultured human umbilical vein endothelial cells (HUVEC)

Pala, Andrea Norcini; D’Elia, Piera; Spampinato, Giuseppe; Pittaluga, Eugenia; Benagiano, Giuseppe

doi:10.3109/14767058.2011.629258

Cited by 4 publications

(2 citation statements)

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“…These findings are important due to the fact that beta-catenin has uterine function and participates in the regulation of endometrial epithelial differentiation during endometrial receptivity period [ 36 , 37 ]. Interestingly, a recent in vitro study demonstrated a link between GdA and the translocation of beta-catenin to promote cell adhesion and formation of adherent junctions through cytoskeletal reorganization in human endothelial cells [ 38 ]. This correlates well with our findings of a significant decrease in both GdA and beta-catenin expression of rFSH treatment.…”

Section: Discussionmentioning

confidence: 99%

Changes in receptivity epithelial cell markers of endometrium after ovarian stimulation treatments: its role during implantation window

et al. 2015

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BackgroundTo compare the expression of receptivity markers in epithelial and stromal cells in the endometrium of ovulatory women and infertile with hypothalamic pituitary dysfunction (HPD), untreated or treated with clomiphene citrate (CC), or with recombinant follicle stimulating hormone (rFSH).MethodsTwelve control ovulatory and 32 anovulatory women, 22 of whom received ovulation induction with CC (n = 12) or rFSH (n = 10). Endometrial biopsies were obtained during the mid-secretory phase. Hormonal secretion was measured by chemiluminescence immunoassay, endometrial dating and cellular expression and distribution of receptivity proteins were evaluated by quantitative immunohistochemistry.ResultsCC or rFSH treatments, modified the expression of epithelial receptivity markers, such as Glycodelin A, beta-catenin, CD166/ALCAM and IGF-1R, but not in stromal markers. Also, a change in their cell distribution was observed.ConclusionsTreatment of infertile women with HPD modified the expression and distribution of receptivity markers in the mid-secretory phase of the endometrium in epithelial but not stromal cells, which can help to explain changes in the receptivity of the endometrium during treatments and suggest an important role of these cells in the receptivity window.Electronic supplementary materialThe online version of this article (doi:10.1186/s12978-015-0034-7) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Changes in receptivity epithelial cell markers of endometrium after ovarian stimulation treatments: its role during implantation window

et al. 2015

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“…Moreover, glycodelin increases VEGF expression in various cancers including endometrial cancer (RL-95 cells), ovarian adenocarcinoma (OVCAR-3 cells), and breast cancer (MCF-7 and MDA-MB-231 cells) ( 57 ). In the process to promote angiogenesis, glycodelin induces more expression of β-catenin in the perimembrane areas of HUVECs in a dose-dependent manner ( 58 ), and upregulated β-catenin can reinforce the intercellular adhesion of HUVECs to facilitate angiogenesis. Solid tumors relies on angiogenesis to spread, invade and metastasize, facilitated by various angiogenic activators like VEGF and bFGF ( 59 ).…”

Section: The Effects Of Glycodelin In Cancer Development and Progressmentioning

confidence: 99%

The Roles of Glycodelin in Cancer Development and Progression

2017

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Glycodelin is a kind of glycoprotein expressed in secretory endometrium, pregnancy deciduas, and amniotic fluid originally, which is vital for the maintenance of normal human reproductive activities. Recent researches have reported that glycodelin is specifically expressed in various malignancies, including female-specific cancers such as endometrial cancer, ovarian cancer and breast cancer, and non-gender specific cancers including lung cancer, and colon cancer, and glycodelin expression correlates with the diagnosis and prognosis of cancer patients. This review focuses on the expression of glycodelin in different cancers and its role in cancer development and progression. Glycodelin possesses the abilities to regulate cancer cell proliferation, differentiation, and invasion, promote cancer angiogenesis, and modulate the differentiation and function of immune cells including T cells, dendritic cells, monocyte-macrophages, natural killer cells and B cells participating in cancer development. The expression of glycodelin can be regulated by stromal cells, lysophosphatidic acid, histone deacetylase inhibitors, and relaxin. In summary, glycodelin is a promising biomarker for the diagnosis and prognosis of cancer patients, and depending on its distinct immunoregulatory effects, glycodelin can be a prospective target for cancer immunotherapy.

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Synovialitis vom arthrofibrotischen Typ

et al. 2012

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After rheumatologic conservative medical therapy has been exhausted in degenerative and inflammatory joint diseases, arthroplastic operations are an important option to restore quality of life. Endoprosthesis-associated arthrofibrosis is a severe fibrosing disease of the synovial membrane after endoprosthetic operations. Neither the morphological substrate nor histopathological criteria have been described. The aim was to describe the histopathological substrate of arthrofibrosis and to define histological and immunohistochemical criteria of arthrofibrosis on the basis of tissue samples derived from revision. In histopathological analyses arthrofibrosis revealed a synovialitis with varying fibrosis, without detectable ossification and without minimal wear particle reaction (so-called synovialitis of arthrofibrotic type, SAT). A 3-stage grading was determined based on the cellular density of the fibrous tissue (fibroblast cellularity). In 191 cases with SAT, grade 1 was found in 24.1 % (n = 46), grade 2 was found in 51.8 % (n = 99) and grade 3 was found in 24.1 % (n = 46). The control group consisted of 29 cases with synovialitis of indifferent type (type IV membrane). If SAT grades 2 and 3 are summed together, i.e. the distance between the fibroblasts was less than two cell lengths, the difference of the fibroblast cellularity compared with the type IV membrane was significant (p < 0.001). Above SAT grade 2 the diagnosis of arthrofibrosis could be made with a sensitivity 0.7592 and specificity 0.8276. The SM-alpha-actin cytoplasmic positivity of fibroblasts indicates a myofibroblast phenotype and the β-catenin positivity suggests a resemblance to fibromatosis or a keloid-like process. In the quantitative evaluation of the β-catenin positive fibroblasts, there was a significant difference (p < 0.001) between type IV membrane and SAT. A threshold value of 20 beta-catenin positive cells per microscopic high power field (HPF) was determined, which represents in conjunction with the clinical information a new histopathological diagnosis component (sensitivity 0.720, specificity 0.867).

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Human amniotic glycodelin actively regulates changes in β-catenin immunoreactivity in cultured human umbilical vein endothelial cells (HUVEC)

Abstract: These data provide further mechanistic insight into the specificity of cell-to-cell adhesion, thus corroborating the role of GdA in promoting angiogenesis.

Cited by 4 publications

References 6 publications

Changes in receptivity epithelial cell markers of endometrium after ovarian stimulation treatments: its role during implantation window

Changes in receptivity epithelial cell markers of endometrium after ovarian stimulation treatments: its role during implantation window

The Roles of Glycodelin in Cancer Development and Progression

Synovialitis vom arthrofibrotischen Typ

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