Human Amniotic Cell Sheet Harvest Using a Novel Temperature-Responsive Culture Surface Coated with Protein-Based Polymer

Zhang, Helin; Iwama, Masamichi; Akaike, Toshihiro; Urry, Dan W.; Pattanaik, Asima; Parker, Timothy M.; Konishi, Ikuo; Nikaido, Toshio

doi:10.1089/ten.2006.12.ft-34

Cited by 5 publications

(7 citation statements)

References 30 publications

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“…In this system, poly-N-isopropylacrylamide (PNIPAM) was widely studied and used as a coating material; however, its cytotoxic properties could not be completely eliminated (86). Therefore we developed a novel culture surface coated with a noncytotoxic, temperature-responsive elastic proteinbased polymer (87). It is also highly biocompatible and imperceptible (the polymer can be injected into the tissue and disappears within 2 weeks without leaving any signs of its presence).…”

Section: Human Amniotic Cell Sheet Usage In the Field Of Tissuementioning

confidence: 99%

The Potential of Amniotic Membrane/Amnion-Derived Cells for Regeneration of Various Tissues

et al. 2007

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Abstract. Regenerative medicine is a new field based on the use of stem cells to generate biological substitutes and improve tissue functions, restoring damaged tissue with high proliferability and differentiability. It is of interest as a potential alternative to complicated tissue / organ transplantation. Recently, amnion-derived cells have been reported to have multipotent differentiation ability, and these cells have attracted attention as a cell source for cell-transplantation therapy. The amnion possesses considerable advantageous characteristics: the isolated cells can differentiate into all three germ layers; they have low immunogenicity and anti-inflammatory functions; and they do not require the sacrifice of human embryos for their isolation, thus avoiding the current controversies associated with the use of human embryonic stem cells. Moreover, we developed human amniotic cell-sheets using a novel culture surface coated with a noncytotoxic, temperature-responsive elastic protein-based polymer. We also generated a "hyperdry-amnion", which has already been applied clinically in the ophthalmological field. Compared to cryopreserved fresh amnion, "hyper-dry-amnion" is easy to handle and has started to bring good results to patients. These materials from the amnion are also expected to open a new field in tissue engineering. Thus, amnion, which had been discarded after parturition, has started to be appreciated as an attractive material in the field of regenerative medicine. In this review, the most recent and relevant clinical and experimental data about the use of amniotic membrane and cells derived from it are described.

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Section: Human Amniotic Cell Sheet Usage In the Field Of Tissuementioning

confidence: 99%

The Potential of Amniotic Membrane/Amnion-Derived Cells for Regeneration of Various Tissues

et al. 2007

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“…Thermoresponsive polymers [31] have been used to recover cell sheets from an elastin polymer-coated inert membrane [46], from cells cultured on poly( N -isopropylacrylamide) (PIPAAm) grafted PEG surfaces [47, 48], or from cells cultured on pluronic-coated surfaces [49]. These novel surfaces allow the cultivation of cells without using enzymes by utilizing the thermoresponsive phase transition property of PNIPAAm [50].…”

Section: Discussionmentioning

confidence: 99%

Engineered extracellular matrices with cleavable crosslinkers for cell expansion and easy cell recovery

2008

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An unmet need for expansion of primary cells and progenitor cells in three dimensions (3-D) is a synthetic mimic of the extracellular matrix (ECM) with user-controllable composition that would permit rapid recovery of viable cells under mild, non-enzymatic conditions. Three block copolymers based on disulfide-containing polyethylene glycol diacrylate crosslinkers were synthesized, and were used to crosslink thiol-modified hyaluronan and gelatin macromonomers in the presence of cells. The triblock PEGSSDA contained a single disulfide-containing block, the pentablock PEG(SS)2DA contained two disulfide blocks, and the heptablock PEG(SS)3DA contained three disulfide blocks. For each hydrogel composition, four cell types were encapsulated in 3-D, and growth and proliferation were evaluated. Murine NIH 3T3 fibroblasts, human HepG2 C3A hepatocytes, human bone-marrow derived mesenchymal stem cells (MSCs), and human umbilical vein endothelial cells (HUVECs) all showed excellent viability and growth during expansion in 3-D in the three disulfide block copolymer crosslinkers. After cell expansion, the hydrogels were dissociated using the thiol-disulfide exchange reaction in the presence of N-acetyl-cysteine or glutathione, which dissolved the hydrogel network. After dissolution, cells were recovered in high yield and with high viability by gentle centrifugation.

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“…They exhibit a reversible lower critical solution temperature (LCST), also known as the inverse transition temperature around 37°C [25], which can be modulated by changes in the ELP sequence and molecular weight. Due to these unique characteristics, ELPs have been studied in depth for applications in drug delivery [26,27], and cell and tissue engineering [28][29][30][31]. The response of ELPs to changes in environmental conditions arises from intramolecular contraction and intermolecular coacervation, both mediated by hydrophobic interactions [24].…”

Section: Introductionmentioning

confidence: 99%

“…Ultrathin coatings based upon ELP conjugates offer tremendous potential to enhance the biocompatibility of substrata such as glass and silicon, rendering them very attractive for biosensor applications. Due to the inverse transition temperature exhibited by ELPs and their inherent biocompatibility, these biopolymers may also be useful for cell-sheet engineering [30]. In previous studies, both polyethyleneimine (PEI) [40][41][42] and polyacrylic acid (PAA) [33,43] have been incorporated in multilayer assemblies to enhance the biocompatibility of surfaces without deleterious cytotoxic effects.…”

Section: Introductionmentioning

confidence: 99%

Cellular response to nanoscale elastin-like polypeptide polyelectrolyte multilayers

et al. 2008

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Human Amniotic Cell Sheet Harvest Using a Novel Temperature-Responsive Culture Surface Coated with Protein-Based Polymer

Cited by 5 publications

References 30 publications

The Potential of Amniotic Membrane/Amnion-Derived Cells for Regeneration of Various Tissues

The Potential of Amniotic Membrane/Amnion-Derived Cells for Regeneration of Various Tissues

Engineered extracellular matrices with cleavable crosslinkers for cell expansion and easy cell recovery

Cellular response to nanoscale elastin-like polypeptide polyelectrolyte multilayers

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