Cellular response to nanoscale elastin-like polypeptide polyelectrolyte multilayers

Swierczewska, Magdalena; Hajicharalambous, C.S.; Janorkar, Amol V.; Megeed, Zaki; Yarmush, Martin L.; Rajagopalan, Padmavathy

doi:10.1016/j.actbio.2007.11.004

Cited by 47 publications

(37 citation statements)

References 55 publications

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“…42 Largely due to their tunable nature, PEMs have had a significant impact in nearly every aspect of biomaterial design and tissue engineering. 10,18,22,24,26,27,30,31,38,[42][43][44][45][46][47][48][49][50][51][52][53][54][55] PEMs have been used to modulate protein adsorption, 33 promote cell adhesion, 16,29,34,38,[55][56][57] or regulate the inflammatory response. [58][59][60] In addition, PEM coatings have the potential to mimic the complex extracellular matrix found in vivo.…”

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confidence: 99%

“…[1][2][3][28][29][30] Synthetic PEMs have been assembled from cationic PEs such as poly (allylamine hydrochloride) (PAH) and poly (diallyldimethylammonium chloride) (PDAC) and anionic PEs such as poly (acrylic acid) (PAA) and sulfonated polystyrene (SPS). 8,29,31,32 PEMs have also been fabricated from naturally occurring PEs such as, polypeptides, polysaccharides, DNA, and proteins.…”

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confidence: 99%

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Polyelectrolyte Multilayers in Tissue Engineering

Detzel

Larkin

Rajagopalan

2011

Tissue Engineering Part B: Reviews

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The layer-by-layer assembly of sequentially adsorbed, alternating polyelectrolytes has become increasingly important over the past two decades. The ease and versatility in assembling polyelectrolyte multilayers (PEMs) has resulted in numerous wide ranging applications of these materials. More recently, PEMs are being used in biological applications ranging from biomaterials, tissue engineering, regenerative medicine, and drug delivery. The ability to manipulate the chemical, physical, surface, and topographical properties of these multilayer architectures by simply changing the pH, ionic strength, thickness, and postassembly modifications render them highly suitable to probe the effects of external stimuli on cellular responsiveness. In the field of regenerative medicine, the ability to sequester growth factors and to tether peptides to PEMs has been exploited to direct the lineage of progenitor cells and to subsequently maintain a desired phenotype. Additional novel applications include the use of PEMs in the assembly of three-dimensional layered architectures and as coatings for individual cells to deliver tunable payloads of drugs or bioactive molecules. This review focuses on literature related to the modulation of chemical and physical properties of PEMs for tissue engineering applications and recent research efforts in maintaining and directing cellular phenotype in stem cell differentiation.

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confidence: 99%

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confidence: 99%

Polyelectrolyte Multilayers in Tissue Engineering

Detzel

Larkin

Rajagopalan

2011

Tissue Engineering Part B: Reviews

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“…For instance, elastin-like polypeptides with .VPGXG/ n peptide sequence have been conjugated to polyethyleneimine and polyacrylic acid polyelectrolytes for further LbL assembly and modulation of in vitro cell adhesion [84].…”

Section: Layer-by-layer Coatingmentioning

confidence: 99%

Biomimetic Tailoring of the Surface Properties of Polymers at the Nanoscale: Medical Applications

Chiono

Descrovi

Sartori

et al. 2010

Scanning Probe Microscopy in Nanoscience and Nanotechnology 2

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New-generation biomaterials are information-rich and incorporate biologically active components derived from Nature. The chapter reviews current approaches for the realization of biomimetic coatings for tissue engineering applications, via functionalization with bioactive molecules such as native long chains of extracellular matrix (ECM) proteins as well as short peptide sequences derived from intact ECM proteins. Biomimetic materials provide cues for cell-matrix interactions, favouring cell adhesion, proliferation, spreading, and differentiation. The recently developed scanning probe techniques for optical and spectroscopic characterization of surfaces are also described, providing advanced topographical imaging and sensitive force measurements. The spectroscopic imaging of surfaces at the nanoscale provides insight into the function and structure of biomimetic molecules and represents a tool for tailoring the surface design.

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“…This is a simple technique for generating bioactive surfaces, driven by complementary forces present between two distinct materials [170]. These ultrathin nanoscale coatings promote cell adhesion and proliferation, and the results show that the thickness and mechanical integrity of the multilayer assembly modulates the cell response.…”

Section: Surface Engineeringmentioning

confidence: 99%