Human amnion epithelial cells assemble tenascins and three fibronectin isoforms in the extracellular matrix

Abstract

Monoclonalantibodies (MAb) were used to show that cultured human amnion epithelial (HuA) cells produce tenascins (Tn) and isoforms of cellular fibronectin (cFn). Tn polypeptides of M, 280,000 and 190,000, assembled into extracellular matrix (ECM) but not secreted into the culture medium by HuA cells, were electrophoretically similar to those produced by human fibroblasts as revealed with domain-specific MAbs. The results suggested that most Fn produced by HuA cells contained the extradomain (ED) A and an oncofetal domain but only a minor fraction EDB. In immunofluorescence Tn and Fn were seen in different cytoplasmic granules upon monensin-Induced intracellular accumulation. Tn appeared to be deposited in the ECM in colocalization with Fn but distinctly slower. The present results show that cultured normal human epithelial cells synthesize Tn and three lsoforms of cFn and secrete them by using different cytoplasmic pathways.

Third Trimester Maternal Plasma Total Fibronectin Levels in Pregnancy-Induced Hypertension: Results of a Tertiary Center

“…Maternal vascular endothelial damage plays a critical role in the development of preeclampsia (16). Because plasma fibronectin is a marker of endothelial damage (5,21), total or cellular fibronectin determinations have been used to differentiate normal and preeclamptic pregnancies (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). In our study, we evaluated ED-B fibronectin, an isoform of fibronectin, which is synthesized in the endothelium of blood vessels and released to the extracellular space at sites of vascular injury.…”

Preservation, sterilization and de-epithelialization of human amniotic membrane for use in ocular surface reconstruction

“…Collagen types I, III, IV, V and VII, laminin (Fig. 2D), and fibronectin have been identified in the amniotic BM [53,54,[63][64][65]. Fukuda Fig.…”

Isolation of fibronectin type III like peptide from human placental extract used as wound healer

“…It has been reported that human amnion epithelial cells secrete cellular (III-A domain containing) and oncofetal (III-B containing) fibronectin isoforms that are assembled in the extracellular matrix [21]. Fibronectins in the amniotic basal lamina, particularly oncofetal isoform, is a part of healing process in this tissue due to the rapidly growing fetus causing partly shears in the amnion [22].…”