Human albumin purification: a modified and concise method

Raoufinia, Ramin; Balkani, Sanaz; Keyhanvar, Neda; Mahdavipor, Behroz; Abdolalizadeh, Jalal

doi:10.1080/15321819.2018.1531884

Cited by 12 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In brief, the solvent can turn albumin β-sheets into α-helices, while breaking intramolecular hydrogen bonds within the β-sheets of the protein and inducing new intermolecular hydrogen interactions [46]. Protein denaturation dynamics are governed by general physical and chemical parameters, like the pH [47], temperature [48], and the kind of the solvent. The system is usually then stabilized via cross-linkers (i.e., glutaraldehyde or 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide; Figure 2B).…”

Section: Synthesis Of Albumin Nanovectorsmentioning

confidence: 99%

Albumin Nanovectors in Cancer Therapy and Imaging

et al. 2019

View full text Add to dashboard Cite

Albumin nanovectors represent one of the most promising carriers recently generated because of the cost-effectiveness of their fabrication, biocompatibility, safety, and versatility in delivering hydrophilic and hydrophobic therapeutics and diagnostic agents. In this review, we describe and discuss the recent advances in how this technology has been harnessed for drug delivery in cancer, evaluating the commonly used synthesis protocols and considering the key factors that determine the biological transport and the effectiveness of such technology. With this in mind, we highlight how clinical and experimental albumin-based delivery nanoplatforms may be designed for tackling tumor progression or improving the currently established diagnostic procedures.

show abstract

Section: Synthesis Of Albumin Nanovectorsmentioning

confidence: 99%

Albumin Nanovectors in Cancer Therapy and Imaging

et al. 2019

View full text Add to dashboard Cite

show abstract

“…92,93 Clinical administration of mAbs well-tolerated normally through intravenous and subcutaneous. 8 The mAbs have several applications including Cancer Immunotherapy, 91,94 Bone Marrow and Organ Transplantation, 95,96 Protein Purification, [97][98][99] Disease Diagnosis in immunoassay tests including enzymelinked immune sorbent assay (ELISA), western blotting, immunohistochemistry and immunofluorescence tests. 100,101 Our team has been developed the mAbs against a wide range of antigens including TNF-α, 102 CD20, 103 CD34, 104,105 receptor tyrosine kinase-like orphan receptor proteins, 106 human epidermal growth factor receptor, 107 heat shock protein 70, 108 vascular endothelial growth factor receptor 2, 77,78 human IgG and IgA.…”

Section: Monoclonal Antibodiesmentioning

confidence: 99%

PCSK9: A Key Target for the Treatment of Cardiovascular Disease (CVD)

et al. 2020

Self Cite

View full text Add to dashboard Cite

Proprotein convertase subtilisin/kexin type 9 (PCSK9), as a vital modulator of low-densitylipoprotein cholesterol (LDL-C) , is raised in hepatocytes and released into plasma where it bindsto LDL receptors (LDLR), leading to their cleavage. PCSK9 adheres to the epidermal growthfactor-like repeat A (EGF-A) domain of the LDLR which is confirmed by crystallography. LDLRexpression is adjusted at the transcriptional level through sterol regulatory element bindingprotein 2 (SREBP-2) and at the post translational stages, specifically through PCSK9, and theinducible degrader of the LDLR PCSK9 inhibition is an appealing new method for reducing theconcentration of LDL-C. In this review the role of PCSK9 in lipid homeostasis was elucidated, theeffect of PCSK9 on atherosclerosis was highlighted, and contemporary therapeutic techniquesthat focused on PCSK9 were summarized. Several restoration methods to inhibit PCSK9 havebeen proposed which concentrate on both extracellular and intracellular PCSK9, and theyinclude blockage of PCSK9 production by using gene silencing agents and blockage of it’sbinding to LDLR through antibodies and inhibition of PCSK9 autocatalytic processes by tinymolecule inhibitors.

show abstract

“…HSA has many important roles such as transporting molecules, conservation of osmotic pressure, stabilizing extracellular fluid volume, capillary membrane permeability, and the effect of neuroprotective items in human plasma. It is also used broadly such as drug delivery, carrying, and transporting molecules in pharmaceutical sciences [ 9 – 17 ]. Many techniques are available for the separation and purification of albumin [ 18 – 22 ].…”

Section: Introductionmentioning

confidence: 99%

Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification

TATAR,

AKGÖNÜLLÜ,

ALAGÖZ

et al. 2023

Turkish Journal of Chemistry

View full text Add to dashboard Cite

Dye-ligand affinity chromatography is among the increasingly popular affinity chromatography based on molecular recognition for the purification of albumin. This study focuses on the binding of Cibacron Blue F3GA ligand dye with magnetic silica particles and purification by separation. Mono-disperse silica particles with bimodal pore size distribution were employed as a high-performance adsorbent for human serum albumin (HSA) protein purification under equilibrium conditions. The synthesized ligand-dye affinity based magnetic silica particles were characterized by electron spin resonance, Fourier-transform infrared spectroscopy, scanning electron microscopy, vibrating sample magnetometer, elemental analysis, and dispersive X-ray analysis. The HSA purification performance of the proposed material in the presence of a magnetic field was relatively investigated using magnetic-based particles with similar morphologies. The maximum adsorption capacity for HSA in an artificial plasma medium was defined as 48.6 mg/g magnetic silica particle. By using the designed magnetic silica particles, 1.0 M NaCl solution was successfully utilized for obtaining quantitative desorption with HSA. However, continued HSA purification performances of magnetic-based particles were significantly lower concerning the ligand-dye magnetic silica particles. The purity of the removed albumin was about 97%. The magnetic silica particles could be utilized many times without decreasing their protein adsorption capacities remarkably.

show abstract

Human albumin purification: a modified and concise method

Cited by 12 publications

References 25 publications

Albumin Nanovectors in Cancer Therapy and Imaging

Albumin Nanovectors in Cancer Therapy and Imaging

PCSK9: A Key Target for the Treatment of Cardiovascular Disease (CVD)

Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification

Contact Info

Product

Resources

About