Albumin Nanovectors in Cancer Therapy and Imaging

Parodi, Alessandro; Miao, Jr‐Ming; Soond, Surinder M.; Rudzińska, Magdalena; Zamyatnin, Andrey A.

doi:10.3390/biom9060218

Cited by 98 publications

(65 citation statements)

References 149 publications

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“…Elevated gp60 and SPARC in the synovium fluid of RA patients and murine CIA model work to transports albumin across the endothelial barrier into the interstitium (Gp60) and SPARC enhances the uptake of albumin into immune cells in the RA synovium 24 , 26 . Albumin-based NPs can be taken up via the SPARC-mediated mechanism 60 and directly internalized by phagocytic cells via interactions with FcRn receptors, or after opsonization by IgG, and eventually sequestered within the endo-lysosomal compartment. The overall scheme of the NPs interactions and internalization within the RA site is presented in Figure 1 C .…”

Section: Resultsmentioning

confidence: 99%

Ceria-based nanotheranostic agent for rheumatoid arthritis

Kalashnikova¹,

Chung²,

Nafiujjaman³

et al. 2020

Theranostics

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Rheumatoid arthritis (RA) is an autoimmune disease that affects 1-2% of the human population worldwide, and effective therapies with targeted delivery for local immune suppression have not been described. We address this problem by developing a novel theranostic nanoparticle for RA and assessed its therapeutic and targeting effects under image-guidance. Methods: Albumin-cerium oxide nanoparticles were synthesized by the biomineralization process and further conjugated with near-infrared, indocyanine green (ICG) dye. Enzymatic-like properties and reactive oxygen species (ROS) scavenging activities, as well as the ability to reprogram macrophages, were determined on a monocyte cell line in culture. The therapeutic effect and systemic targeting potential were evaluated in collagen-induced arthritis (CIA) mouse model using optical/optoacoustic tomographic imaging. Results: Small nanotheranostics with narrow size distribution and high colloidal stability were fabricated and displayed high ROS scavenging and enzymatic-like activity, as well as advanced efficacy in a converting pro-inflammatory macrophage phenotype into anti-inflammatory phenotype. When administrated into affected animals, these nanoparticles accumulated in inflamed joints and revealed a therapeutic effect similar to the gold-standard therapy for RA, methotrexate. Conclusions: The inflammation-targeting, inherent contrast and therapeutic activity of this new albumin-cerium oxide nanoparticle may make it a relevant agent for assessing severity in RA, and other inflammatory diseases, and controlling inflammation with image-guidance. The design of these nanotheranostics will enable potential clinical translation as systemic therapy for RA.

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Section: Resultsmentioning

confidence: 99%

Ceria-based nanotheranostic agent for rheumatoid arthritis

Kalashnikova¹,

Chung²,

Nafiujjaman³

et al. 2020

Theranostics

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“…As well known, SPARC, owing to its albumin binding properties, could promote the accumulation of albumin in cancer cells and tissues ( Tai and Tang, 2008 ; Bannunah et al, 2014 ; Bairagi et al, 2015 ; Cheng et al, 2017 ; Parodi et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Albumin-Modified Melanin-Silica Hybrid Nanoparticles Target Breast Cancer Cells via a SPARC-Dependent Mechanism

Sanità

Armanetti

Silvestri

et al. 2020

Front. Bioeng. Biotechnol.

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Bioconjugation of a recently developed photoacoustic nanoprobe, based on silica-templated eumelanin-silver hybrid nanoparticles (MelaSil_Ag-NPs), with human serum albumin (HSA) is disclosed herein as an efficient and practical strategy to improve photostability and to perform SPARC mediated internalization in breast cancer cells. Modification of NPs with HSA induced a slight viability decrease in breast cancer cells (HS578T) and normal breast cells (MCF10a) when incubated with HSA-NPs up to 100 μg/mL concentration for 72 h and a complete suppression of hemotoxicity for long incubation times. Uptake experiments with MelaSil_Ag-HSA NPs indicated very high and selective internalization via SPARC in HS578T (SPARC positive cells) but not in MCF10a (SPARC negative cells), as evaluated by using endocytosis inhibitors. The binding of SPARC to HSA was confirmed by Co-IP and Dot-blot assays. Additional studies were performed to analyze the interaction of MelaSil_Ag-HSA NPs with protein corona. Data showed a dramatic diminution of interacting proteins in HSA conjugated NPs compared to bare NPs. HSA-coated MelaSil_Ag-NPs are thus disclosed as a novel functional nanohybrid for potential photoacoustic imaging applications.

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“…Other types of detected modifications are questionable. It has been reported that phosphorylation of ALBU can occur non-specifically and, thus, create non-naturally originated identifications in proteomic assays [ 38 ]. On the other hand, enhanced non-specific phosphorylation of proteins, including ALBU in warfarin-binding site [ 39 ], is a known effect of the impaired cell signaling network, which significantly contributes in tumor growth and development [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

Super Secondary Structures of Proteins with Post-Translational Modifications in Colon Cancer

et al. 2020

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New advances in protein post-translational modifications (PTMs) have revealed a complex layer of regulatory mechanisms through which PTMs control cell signaling and metabolic pathways, contributing to the diverse metabolic phenotypes found in cancer. Using conformational templates and the three-dimensional (3D) environment investigation of proteins in patients with colorectal cancer, it was demonstrated that most PTMs (phosphorylation, acetylation, and ubiquitination) are localized in the supersecondary structures (helical pairs). We showed that such helical pairs are represented on the outer surface of protein molecules and characterized by a largely accessible area for the surrounding solvent. Most promising and meaningful modifications were observed on the surface of vitamin D-binding protein (VDBP), complement C4-A (CO4A), X-ray repair cross-complementing protein 6 (XRCC6), Plasma protease C1 inhibitor (IC1), and albumin (ALBU), which are related to colorectal cancer developing. Based on the presented data, we propose the impact of the observed modifications in immune response, inflammatory reaction, regulation of cell migration, and promotion of tumor growth. Here, we suggest a computational approach in which high-throughput analysis for identification and characterization of PTM signature, associated with cancer metabolic reprograming, can be improved to prognostic value and bring a new strategy to the targeted therapy.

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Albumin Nanovectors in Cancer Therapy and Imaging

Cited by 98 publications

References 149 publications

Ceria-based nanotheranostic agent for rheumatoid arthritis

Ceria-based nanotheranostic agent for rheumatoid arthritis

Albumin-Modified Melanin-Silica Hybrid Nanoparticles Target Breast Cancer Cells via a SPARC-Dependent Mechanism

Super Secondary Structures of Proteins with Post-Translational Modifications in Colon Cancer

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