Human Adipose-Derived Mesenchymal Stromal/Stem Cell Spheroids Possess High Adipogenic Capacity and Acquire an Adipose Tissue-like Extracellular Matrix Pattern

Hoefner, Christiane; Muhr, Christian; Horder, Hannes; Wiesner, Miriam; Wittmann, Katharina; Lukaszyk, Daniel; Radeloff, Katrin; Winnefeld, Marc; Becker, Matthias; Blunk, Torsten; Bauer‐Kreisel, Petra

doi:10.1089/ten.tea.2019.0206

Cited by 33 publications

(36 citation statements)

References 57 publications

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“…Adipose tissue ECM primarily consists of collagen, laminin, fibronectin, and elastin (Divoux et al, 2010). Hoefner et al showed that hASCs produce this ECM‐like pattern when cultured as 3D spheroids (Höfner et al, 2020). In this study, we chose to use the ELP‐based coating to induce 3D spheroid formation.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the formation, adipogenic maturation, and retention of human adipose‐derived stem cell spheroids in scaffold‐free culture techniques

2020

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While three‐dimensional spheroids outperform traditional two‐dimensional monolayer culture for human adipose‐derived stem cells (hASCs), there is not a consensus on the most successful method for enhancing their adipogenic differentiation and minimizing the loss of physiologically relevant, fatty spheroids during culture. To this end, we compared three culture methods, namely, elastin‐like polypeptide‐polyethyleneimine (ELP‐PEI) coated surfaces, ultra‐low attachment static culture, and suspension culture for their ability to form and retain productive hASC spheroids. The ELP‐PEI coatings used the ELP conjugated to two molecular weights of PEI (800 or 25,000 g/mol). FTIR spectroscopy, atomic force microscopy, and contact angle goniometry revealed that the ELP‐PEI coatings had similar chemical structures, surface topography, and hydrophobicity. Time‐lapse microscopy showed that increasing the PEI molecular weight resulted in smaller spheroids. Measurement of triglyceride content showed that the three methods induced comparable differentiation of hASCs toward the adipogenic lineage. DNA content and morphometric analysis revealed merging of spheroids to form larger spheroids in the ultra‐low attachment static culture and suspension culture methods. In contrast, the retention of hASC spheroid sizes and numbers with a regular spheroid size (~100 μm) were best atop the ELP‐PEI800 coatings. Overall, this research shows that the spheroid culture atop the ELP‐PEI coatings is a suitable cell culture model for future studies involving long‐term, three‐dimensional culture of mature adipocytes derived from hASCs.

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Section: Discussionmentioning

confidence: 99%

Comparison of the formation, adipogenic maturation, and retention of human adipose‐derived stem cell spheroids in scaffold‐free culture techniques

2020

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“…These results indicate that spheroid formation enhanced the functions of m17.ASC cells, as previously reported in MSC spheroid formation. [19] 3.3. Tissue distribution of m17.ASC/Nluc spheroids and suspended m17.ASC/Nluc cells in mice Figure 4A shows the tissue distribution of m17.ASC/Nluc spheroids and suspended m17.ASC/Nluc cells 24 h after intravenous injection in mice.…”

Section: Characteristics Of M17asc Spheroidsmentioning

confidence: 99%

Intravenous injection of mesenchymal stem cell spheroids for efficient pulmonary delivery and prolonged in vivo survival

Shimazawa¹,

Kusamori²,

Tsujimura³

et al. 2021

Preprint

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Because of their excellent therapeutic potential, mesenchymal stem cells (MSCs) have been used as cell therapeutics for various diseases. However, the survival rate and duration of MSCs after transplantation are extremely low and short, respectively. To solve these problems, in this study, we prepared multicellular spheroids of MSCs and investigated their survival and function after intravenous injection in mice. The murine adipose-derived MSC line m17.ASC was cultured in agarose-based microwell plates to obtain size-controlled m17.ASC spheroids of an average diameter and cell number of approximately 170 μm and 1100 cells/spheroid, respectively. The intravenously injected m17.ASC spheroids mainly accumulated in the lung and showed a higher survival rate than suspended m17.ASC cells during the experimental period of 7 days. m17.ASC spheroids efficiently reduced the lipopolysaccharide-induced increase in plasma concentrations of interleukin-6 and tumor necrosis factor-α. These results indicate that spheroid formation improved the pulmonary delivery and survival of MSCs, as well as their therapeutic potential against inflammatory pulmonary diseases.

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“…The adipose-derived mesenchymal stromal/stem cells exhibited enhanced adipogenic capacity and generates adipose-like microtissues, which may be a promising cell delivery strategy for adipose tissue engineering approaches. 121,122 Stem cells can also differentiate in to many cell types. After stem cells were made into spheroids, growth factors were added to allow the desired aspect of differentiation.…”

Section: Tissue Engineering Blocksmentioning

confidence: 99%

Advanced biomedical applications based on emerging 3D cell culturing platforms

2020

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Human Adipose-Derived Mesenchymal Stromal/Stem Cell Spheroids Possess High Adipogenic Capacity and Acquire an Adipose Tissue-like Extracellular Matrix Pattern

Cited by 33 publications

References 57 publications

Comparison of the formation, adipogenic maturation, and retention of human adipose‐derived stem cell spheroids in scaffold‐free culture techniques

Comparison of the formation, adipogenic maturation, and retention of human adipose‐derived stem cell spheroids in scaffold‐free culture techniques

Intravenous injection of mesenchymal stem cell spheroids for efficient pulmonary delivery and prolonged in vivo survival

Advanced biomedical applications based on emerging 3D cell culturing platforms

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