Human acid ceramidase is overexpressed but not mutated in prostate cancer

A Guanidine-Based Synthetic Compound Suppresses Angiogenesis via Inhibition of Acid Ceramidase

“…The expression level of ASAH1 was investigated in three normal cell lines (HUVECs, HEK239, and Chang) and six cancer cell lines (HepG2, Huh7, U87MG, H1299, HCT116, and HeLa) to establish a correlation between E2 and its potential target protein ASAH1. As a result, the expression level of ASAH1 in cancer cells was higher than that of normal cells, which is consistent with recent reports (Figure a and b). , These nine cell lines were also treated with E2 (from 0 to 80 μM), and cell proliferation was monitored for 72 h to obtain IC 50 value of E2 (Supplementary Table 1, Supplementary Figures 8 and 9). In addition, Pearson correlation coefficient was used to study the relationship between ASAH1 expression and E2 efficacy.…”

A Guanidine-Based Synthetic Compound Suppresses Angiogenesis via Inhibition of Acid Ceramidase

“…The expression level of ASAH1 was investigated in three normal cell lines (HUVECs, HEK239, and Chang) and six cancer cell lines (HepG2, Huh7, U87MG, H1299, HCT116, and HeLa) to establish a correlation between E2 and its potential target protein ASAH1. As a result, the expression level of ASAH1 in cancer cells was higher than that of normal cells, which is consistent with recent reports (Figure a and b). , These nine cell lines were also treated with E2 (from 0 to 80 μM), and cell proliferation was monitored for 72 h to obtain IC 50 value of E2 (Supplementary Table 1, Supplementary Figures 8 and 9). In addition, Pearson correlation coefficient was used to study the relationship between ASAH1 expression and E2 efficacy.…”

Discovery of highly potent acid ceramidase inhibitors with in vitro tumor chemosensitizing activity

“…By contrast, incubation of SW403 cells with 5-FU (3 μM, 3 h) did not cause any detectable changes in AC activity or ceramide content (Figure 2A–C). Similar results were obtained with LNCaP cells, an androgen-sensitive human prostate adenocarcinoma cell line that overexpresses AC8 (Figure 2D–F and Supplementary Table S2). Furthermore, systemic administration of carmofur (10 or 30 mg-kg −1 , intraperitoneal, i.p.)…”

Combined therapeutic use of AdGFPFasL and small molecule inhibitors of ceramide metabolism in prostate and head and neck cancers: a status report

“…We now know by Western blotting that 67% of our prostate tumor samples have upregulated expression of acid ceramidase while 70% of HNSCC do (Figure 3a and b). Thus, these preliminary data substantiate the earlier observation by Seelan et al 36 that many of these tumors have the potential for exhibiting an anti-apoptotic phenotype via deacylation of ceramide by lysosomal acid ceramidase.…”

A Guanidine-Based Synthetic Compound Suppresses Angiogenesis via Inhibition of Acid Ceramidase

“…The expression level of ASAH1 was investigated in three normal cell lines (HUVECs, HEK239, and Chang) and six cancer cell lines (HepG2, Huh7, U87MG, H1299, HCT116, and HeLa) to establish a correlation between E2 and its potential target protein ASAH1. As a result, the expression level of ASAH1 in cancer cells was higher than that of normal cells, which is consistent with recent reports (Figure a and b). , These nine cell lines were also treated with E2 (from 0 to 80 μM), and cell proliferation was monitored for 72 h to obtain IC 50 value of E2 (Supplementary Table 1, Supplementary Figures 8 and 9). In addition, Pearson correlation coefficient was used to study the relationship between ASAH1 expression and E2 efficacy.…”

Discovery of highly potent acid ceramidase inhibitors with in vitro tumor chemosensitizing activity

“…By contrast, incubation of SW403 cells with 5-FU (3 μM, 3 h) did not cause any detectable changes in AC activity or ceramide content (Figure 2A–C). Similar results were obtained with LNCaP cells, an androgen-sensitive human prostate adenocarcinoma cell line that overexpresses AC8 (Figure 2D–F and Supplementary Table S2). Furthermore, systemic administration of carmofur (10 or 30 mg-kg −1 , intraperitoneal, i.p.)…”

Combined therapeutic use of AdGFPFasL and small molecule inhibitors of ceramide metabolism in prostate and head and neck cancers: a status report

“…We now know by Western blotting that 67% of our prostate tumor samples have upregulated expression of acid ceramidase while 70% of HNSCC do (Figure 3a and b). Thus, these preliminary data substantiate the earlier observation by Seelan et al 36 that many of these tumors have the potential for exhibiting an anti-apoptotic phenotype via deacylation of ceramide by lysosomal acid ceramidase.…”

A Guanidine-Based Synthetic Compound Suppresses Angiogenesis via Inhibition of Acid Ceramidase

“…The expression level of ASAH1 was investigated in three normal cell lines (HUVECs, HEK239, and Chang) and six cancer cell lines (HepG2, Huh7, U87MG, H1299, HCT116, and HeLa) to establish a correlation between E2 and its potential target protein ASAH1. As a result, the expression level of ASAH1 in cancer cells was higher than that of normal cells, which is consistent with recent reports (Figure a and b). , These nine cell lines were also treated with E2 (from 0 to 80 μM), and cell proliferation was monitored for 72 h to obtain IC 50 value of E2 (Supplementary Table 1, Supplementary Figures 8 and 9). In addition, Pearson correlation coefficient was used to study the relationship between ASAH1 expression and E2 efficacy.…”

Discovery of highly potent acid ceramidase inhibitors with in vitro tumor chemosensitizing activity

“…By contrast, incubation of SW403 cells with 5-FU (3 μM, 3 h) did not cause any detectable changes in AC activity or ceramide content (Figure 2A–C). Similar results were obtained with LNCaP cells, an androgen-sensitive human prostate adenocarcinoma cell line that overexpresses AC8 (Figure 2D–F and Supplementary Table S2). Furthermore, systemic administration of carmofur (10 or 30 mg-kg −1 , intraperitoneal, i.p.)…”

Combined therapeutic use of AdGFPFasL and small molecule inhibitors of ceramide metabolism in prostate and head and neck cancers: a status report

“…We now know by Western blotting that 67% of our prostate tumor samples have upregulated expression of acid ceramidase while 70% of HNSCC do (Figure 3a and b). Thus, these preliminary data substantiate the earlier observation by Seelan et al 36 that many of these tumors have the potential for exhibiting an anti-apoptotic phenotype via deacylation of ceramide by lysosomal acid ceramidase.…”