Huh-7: A human “hemochromatotic” cell line

Vecchi, Chiara; Montosi, Giuliana; Pietrangelo, Antonello

doi:10.1002/hep.23410

Cited by 42 publications

(34 citation statements)

References 22 publications

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“…All of the above observations were found to occur independent of p53 status of the cells. We also observed potent cytotoxicity of TSC24 in Huh7 cells (data not shown), another HCC cell line carrying a HFE mutation similar to that associated with human HH (43). Our findings suggest that TSC24 exerts its anticancer effects in a HFE-independent manner.…”

Section: Discussionsupporting

confidence: 59%

Iron Deprivation Suppresses Hepatocellular Carcinoma Growth in Experimental Studies

Hao

Huang

et al. 2011

Clinical Cancer Research

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Purpose: Hepatocellular carcinoma (HCC) is the third most common cause of cancer-related death, and iron overload is a significant risk factor in the development of HCC. In this study, we investigated the potential application of depriving iron by a novel iron chelator, thiosemicarbazone-24 (TSC24), in HCC treatment.Experimental Design: Two HCC cell lines and HFE knockout (HFE À/À ) mice were used to determine iron chelation efficiency of TSC24. The anticancer effects of TSC24 on HCC were analyzed in vitro and in athymic xenograft mouse models.Results: Treatment with TSC24 significantly decreased the cellular iron concentration in hepatoma cells and the serum iron concentration in HFE À/À mice by blocking iron uptake and interfering with normal regulation of iron levels. Moreover, the viability of HCC cell lines was reduced by TSC24. Confirming the mechanism of the agent, this decrease in viability could be partially rescued by addition of exogenous iron. TSC24 also suppressed tumor growth in athymic mice bearing human HCC xenografts in a concentration-dependent manner, without apparent toxicity in parallel with a decrease in the serum iron level. Further studies revealed that TSC24 efficiently triggered cell-cycle arrest and apoptosis in Hep3B and HepG2 cell lines. Conclusions: TSC24 is a potent iron chelator that suppresses human HCC tumor growth by disrupting iron homeostasis, reducing available iron, and triggering cell-cycle arrest and apoptosis, without apparent host toxicity at effective doses. Thus, TSC24 shows great potential for the treatment of HCC. Clin Cancer Res; 17(24); 7625-33. Ó2011 AACR.

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Section: Discussionsupporting

confidence: 59%

Iron Deprivation Suppresses Hepatocellular Carcinoma Growth in Experimental Studies

Hao

Huang

et al. 2011

Clinical Cancer Research

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“…Huh-7 cells are hepatic carcinoma cells which are very useful for primary drug screening in vitro. 15 We observed that both the EA and ME extracts of MP seed possessed antiproliferative action at lower concentration (1 mg/ml) whereas the PE extract had no such effect. The PE extract was not examined in the other assays due to its poor antiproliferative activity.…”

Section: Discussionmentioning

confidence: 70%

Cytotoxicity of different extracts of Mucuna pruriensseeds on hepatoma cells but not on normal hepatic cells

Yadav¹,

Singh²,

Sarkar³

et al. 2014

Phcog Commn.

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Object: Cancer has become leading cause of death in India as well as world. Synthetic anticancer agents have costly and highly toxic to human body. Therefore, it is necessary to discover new therapeutic agents from natural origin for this disease. Materials and Methods: Mucuna pruriens Linn. (MP) (family Fabaceae), is widely used in folk medicine and there is no any such report on antiproliferative effects of this plant. The first objective of this study is to investigate the anticancer properties using hepatoma cells of MP seeds. The first objective of this study is to investigate the anticancer properties of MP seeds by using/on hepatoma cells. Secondly, it is necessary to explore its hepatotoxicity potential in normal cells as well. Therefore, the other objective of this study is to investigate the toxicity using normal hepatic cells. To achieve this goal, hot extraction of MP seeds were performed using petroleum ether 60-80°C (PE), ethyl acetate (EA) and Methanol (ME) as a solvent. All the three extracts were subjected to screen for anti-proliferative action using human hepatoma cell line (Huh-7 cells) at different concentrations from 0.5 to 100 µg/ml, followed by apoptotic activity using caspase-3 enzymes. On the other hand, hepatoprotective activity was performed using human primary hepatic cells (THLE-2 cells). Results: Our result collectively suggested that both EA and ME extract possessed anti-proliferative action on Huh-7 cells and less cytotoxic action on normal cells. Conclusion: Among these two extracts, it is demonstrated for the first time that ME extracts of MP seeds have cytotoxic effect on hepatoma cells but not on normal hepatic cells.

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“…These replicon cells were derived from the Huh7 cell line. Huh7 cells carry an HFE mutation (Y231 del), which is associated with human hemochromatosis [33]. As a conesquence of the significant problems associated with using HCV replicon to investigate iron-mediated processes, HepG2 cells treated with DEM were used as the model system in our study.…”

Section: Discussionmentioning

confidence: 99%

Effect of Iron-Mediated Oxidative Stress on Insulin Resistance Through the Forkhead Box-Containing Protein O Subfamily-1 (FOXO-1) Pathway in Chronic Hepatitis C

Kobayashi¹,

Iwasa²,

Miyachi³

et al. 2013

IJCM

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Aims: Chronic hepatitis C virus (HCV) infection is often associated with glucose metabolic disorders and iron overload. It has recently been shown that reactive oxygen species (ROS) increase gluconeogenesis in hepatocytes through the forkhead box-containing protein O subfamily-1 (FOXO1)-dependent pathway. The aim of this study is proving a causeand-effect relationship between iron-mediated ROS production and insulin resistance (IR) in chronic hepatitis C (CH-C) patients. Methods: The study included 42 patients with CH-C (22 males and 20 females, median age 53 years). Homeostasis model assessment of insulin resistance (HOMA-IR) value was assessed for each patient at entry. Gene expression levels in the biopsied liver tissues were determined by quantitative reverse transcription-polymerase chain reaction (RT-PCR). In addition, the effect of ROS on gluconeogenesis was assessed using HepG2 cells treated with a well-known ROS generator, diethylmaleate (DEM). Results: The serum ferritin levels were significantly correlated with the serum aspartate aminotransferase level, alanine aminotransferase level, HOMA-IR value, grade of fatty accumulation, total hepatic iron score, and 8-OH-deoxy-2'-guanosine (8-OHdG)-positive cell count. FOXO1 expression was correlated with 8-OHdG-positive cell count, phosphoenolpyruvate carboxykinase (PEPCK) expression, and HOMA-IR. In HepG2 cells, the gene transcription of FOXO1 and PEPCK was increased by DEM treatment, which was associated with an increase in non-phosphorylated FOXO1 protein in the nuclear fraction. Conclusions: Iron-mediated ROS production enhances gluconeogenesis through the FOXO1-mediated pathway and is an affecting factor to IR in patients with CH-C.

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Huh-7: A human “hemochromatotic” cell line

Cited by 42 publications

References 22 publications

Iron Deprivation Suppresses Hepatocellular Carcinoma Growth in Experimental Studies

Iron Deprivation Suppresses Hepatocellular Carcinoma Growth in Experimental Studies

Cytotoxicity of different extracts of Mucuna pruriensseeds on hepatoma cells but not on normal hepatic cells

Effect of Iron-Mediated Oxidative Stress on Insulin Resistance Through the Forkhead Box-Containing Protein O Subfamily-1 (FOXO-1) Pathway in Chronic Hepatitis C

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