Cytotoxicity of different extracts of Mucuna pruriensseeds on hepatoma cells but not on normal hepatic cells

Abstract

Object: Cancer has become leading cause of death in India as well as world. Synthetic anticancer agents have costly and highly toxic to human body. Therefore, it is necessary to discover new therapeutic agents from natural origin for this disease. Materials and Methods: Mucuna pruriens Linn. (MP) (family Fabaceae), is widely used in folk medicine and there is no any such report on antiproliferative effects of this plant. The first objective of this study is to investigate the anticancer properties using hepatoma cells of MP seeds. The first objective of this study is to investigate the anticancer properties of MP seeds by using/on hepatoma cells. Secondly, it is necessary to explore its hepatotoxicity potential in normal cells as well. Therefore, the other objective of this study is to investigate the toxicity using normal hepatic cells. To achieve this goal, hot extraction of MP seeds were performed using petroleum ether 60-80°C (PE), ethyl acetate (EA) and Methanol (ME) as a solvent. All the three extracts were subjected to screen for anti-proliferative action using human hepatoma cell line (Huh-7 cells) at different concentrations from 0.5 to 100 µg/ml, followed by apoptotic activity using caspase-3 enzymes. On the other hand, hepatoprotective activity was performed using human primary hepatic cells (THLE-2 cells). Results: Our result collectively suggested that both EA and ME extract possessed anti-proliferative action on Huh-7 cells and less cytotoxic action on normal cells. Conclusion: Among these two extracts, it is demonstrated for the first time that ME extracts of MP seeds have cytotoxic effect on hepatoma cells but not on normal hepatic cells.

6,7-Dimethoxy-1,2,3,4-Tetrahydro-Isoquinoline-3-Carboxylic Acid Attenuates Heptatocellular Carcinoma in Rats with NMR-Based Metabolic Perturbations

“…While searching for new HCC agents, our previous study unveiled that the MP seeds had good antiproliferative action on Huh-7 cells [ 8 ] and this plant is rich with isoquinoline alkaloid [ 9 ]. In another of our studies, one isoquinoline alkaloid, namely M1, isolated from MP seeds, showed good antiproliferative action in vitro on Huh-7 cells through 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyl tetrazolium bromide (MTT) assay [ 12 ].…”

“…Natural products have been used for the treatment of hepatic cancer from ancient times but very few compounds isolated till date from natural origin are effective against hepatic cancer [ 7 ]. Our previous studies demonstrated that the ethyl acetate and methanol extracts of Mucuna pruriens (MP) seeds have potent antiproliferative action on human hepatoma cell line (Huh-7 cells) [ 8 ]. Recent investigation revealed that the seeds of MP are rich with isoquinoline alkaloids [ 9 ] and these alkaloids have a dramatic role in anticancer therapy [ 10 , 11 ].…”

Preclinical Evaluation of Dimethyl Itaconate Against Hepatocellular Carcinoma via Activation of the e/iNOS-Mediated NF-κB–Dependent Apoptotic Pathway

“…The enzyme levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) were measured in serum by using commercially available kits (Yadav et al, 2015;Chauhan et al, 2017;Rai et al, 2018). Catabolic byproducts, including biliverdin and bilirubin, were identified in the liver tissue (Miranda et al, 2001).…”

Phytochemical Analysis, Antioxidant and Cytotoxic Activities of Mucuna pruriens Leaves

“…Ethyl acetate and methanol extracts of M. pruriens inhibited the proliferation of hepatoma cells in vitro with a concentration at 1 μg/mL. 23 However, another study reported that the aqueous and ethanolic extracts of M. pruriens seed has no toxicity to human cervix adenocarcinoma (HeLa) cells when tested using 50 µg/mL of it. 24 Reactive oxygen and nitrogenous species generated from cellular metabolism are detrimental to our health.…”