Hubungan Kekerabatan Genetik Antar Tanaman The F1 dari Persilangan TRI 2024 X PS 1 Berdasarkan Penanda RAPD

Sriyadi, Bambang; Setiamihardja, Ridwan; Baihaki, Achmad; Astika, W.

doi:10.24198/zuriat.v13i1.6715

Cited by 3 publications

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“…Metode tersebut telah diaplikasikan pada plasma nutfah beberapa jenis tanaman perkebunan, antara lain karet (Venkatachalam et al, 2002;Venkatachalam, Priya, Saraswathy-Amma, & Thulaseedharan, 2004;Zewei, Han, Aihua, Jianlin, & Huasun, 2005;Oktavia & Lasminingsih, 2011), kopi (Dinesh, Shivanna, & Ram, 2011;Kathurima, Kenji, Muhoho, Gichimu, & Gichuru, 2012), kakao (Russell, Hosein, Johnson, Waugh, & Powell, 1993;Syafaruddin & Nasution, 2012;Rahmansyah, Mutmainah, Muslimin, & Suwastika, 2014;Panggeso, Anshary, Samudin, & Basri, 2015), dan beberapa tanaman lainnya. Sementara itu, karakterisasi secara molekuler menggunakan teknik RAPD pada tanaman teh telah dilaporkan oleh Sriyadi, Setiamihardja, Baihaki, & Astika (2002), Cheng-Wen et al (2008), Goonetilleke, Priyantha, Mewan, & Gunasekare ( 2009), Mishra, Chaudhary, Ahmad, & Siddiqi ( 2009), Boonerjee, Islam, Hoque, & Sarker (2014), Martono & Udarno (2014). Informasi genetik yang diperoleh melalui analisis molekuler pada penelitian ini dapat dijadikan sebagai dasar pertimbangan dalam pemilihan materi genetik.…”

Section: Pendahuluanunclassified

Genetic Variability of 21 Tea Genotypes [Camellia sinensis (L.) O. Kuntze] Based on RAPD Markers

Martono¹,

Syafaruddin²

2018

J. tanam. industri dan penyegar

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<em>Knowing the genetic diversity in the tea germplasms collection is one of important conditions for assembling new superior varieties. Information of genetic diversity can be obtained through analysis using RAPD molecular markers. The study aimed to determine the genetic diversity of 21 tea genotypes based on RAPD markers. The research was conducted in Integrated Laboratory, Seameo Biotrop, Bogor, from July to September 2013. Genomic DNA was isolated from 21 tea genotypes leaf samples, then amplified with primer OPA 03, OPA 05, OPB 04, OPB 06, OPC 06, and OPD 08. Electrophoresis result was converted into binary data. The genetic similarity and cluster analysis calculation was done using NTSYS-pc version 2.10. In this research, 50 polymorphic bands (94,34%) and 3 monomorphic band (5,66%) were obtained. Cluster analysis based on Nei's genetic distance using the unweighted pair-group method with arithmatic (UPGMA) divided 21 tea genotypes into two groups at a genetic similarity value of 0,48. Group 1 consisted of 20 tea genotypes, while the second group comprised only a one genotype (Sin 27). The range of genetic similarity matrix was between 28%–92%, the lowest genetic similarity (28%) was found between GMB 4 and Sin 27 genotypes, while the highest (92%) was found between AS 2 and AS 1 genotypes. The information obtained can be utilized in breeding programs with the support of agronomic characters as well as in the conservation of tea germplasm.</em>

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Section: Pendahuluanunclassified

Genetic Variability of 21 Tea Genotypes [Camellia sinensis (L.) O. Kuntze] Based on RAPD Markers

Martono¹,

Syafaruddin²

2018

J. tanam. industri dan penyegar

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“…Analisis hubungan kekerabatan genetik tanaman teh menggunakan penanda RAPD dan ISSR yang dilakukan oleh Lai et al, (2001) berhasil mengelompokkan tiga varietas yang ada di Taiwan. Sriyadi et al, (2002) melaporkan hubungan kekerabatan genetik menggunakan penanda RAPD pada tanaman F1 persilangan TRI 2024 x PS 1 ternyata empat klon dekat dengan TRI 2024 dan 41 klon dekat dengan PS 1. Varietas atau klon yang memiliki hubungan kekerabatan dekat pada umumnya mempunyai ting tingkat kemiripan yang tinggi sehingga dalam memilih aksesi koleksi diharapkan dapat diwakili salah satu atau beberapa klon dengan tetap mempertahankan keragaman genetik yang tinggi.…”

Section: Pendahuluanunclassified

Analysis of leaves morphology similarity of several first generation tea clones

Sriyadi

2016

JSTK

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As an effort to conserve tea clone accessions of first generation from genetic erosion, arrangement germplasm in one particular block/area to be easily monitored and managed, is needed. Ideally, tea germplasm collection should consist of a few clone accession number but high genetic variability. To select representative clones that will be conserved in new germplasm collection, leaves morphology similarity has been analysed by cluster analysis using average method. The results showed that by using leaves morphology parameter, from 50 tea clone accession of first generation coming from several tea plantations, it could be reduced into 25 clone accessions from seven clusters, which each has high similarity. The first cluster with 60% similarity could reduced accession from 15 to 6 clone. The second with 74,8% similarity from 25 to 13 clones. The third with 74,8% similarity, from 4 to 2 clones. The fourth with 61,3% similarity, and seventh with 82,1% similarity, both could reduce from 2 clones to 1 clone. To design a tea germplasm collection with 50 accessions of genetic variability, it could represented by 25 clones with low similarity, i.e PG 3, KP 1, Skm 18, Cin 69, Cin 67 dan Cin 58 as the first cluster representatives, Mel 101, PG 4, Pam 15, PG 5,Â PG 11, GP 2, BD 13, SA 49, Cin 66, Mal 16, Bks 6 and Sin 5 the second cluster representatives;Â Â Mel 108 and Cin 13 as the third cluster representatives; and Cin 15, Cip 37, GH 5, as well as GP 4 as the fourth, the fifth, and the sixth representatives, respectively.

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“…Gambar 2 memperlihatkan bahwa pada tingkat kemiripan 46,79% klon-klon teh generasi pertama dapat dibedakan secara tegas dengan klon-klon teh generasi kedua, meskipun gen-gen klon generasi kedua sebagian besar berasal dari klon-klon generasi pertama, terutama klon PS 1 yang merupakan salah satu tetua klon generasi kedua. Hal ini disebabkan adanya efek heterosis pada tanaman teh Sriyadi, 1995) sehingga tanaman generasi F 1 memiliki karakter yang melebihi kedua tetua. Pada tingkat kemiripan 15,60%, ternyata terdapat 6 (enam) kelompok klon yang memiliki hubungan genetik terpisah.…”

Section: Figureunclassified

An assessment of genetic relationship in tea clones based on important chemical compound and yield potential

Sriyadi

2016

JSTK

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Assembling of new excellence high yielÂding and good quality tea clones was started from identification accession as gene sources, crossing design of parents, selection mother bushes and propagated vegetatively. Crossing beetwen two parent clones which near or remote genetic relationship in tea will failed. Assessment of genetic relationship clone accession was done using secondary datas from description of first and second tea clone geneÂration which cultivated in Indonesia. Data of yield potentials and main chemical comÂpound of quality among others catechine, gallat cateÂchine, catechine gallo and cafeine were anaÂlysed using biplot and cluster analysis. The results showed that no correllation beetwen main chemical compound of quality and yield potentials. Biplot with two dimensions could be explained 92,5% variation of five characters and showed that yield has the highest and catechine the smallest variances. The first geneÂration of tea clones was separated to second at the 46,79% similarity levels. At the 15,60% similarity levels, there were six clusters. GMB 1, GMB 2, GMB 6, GMB 9, and GMB 10 clones were in one cluster was caused of they had high of yield and catechine. GMB 4 and GMB 5 had similarity on medium yields, GMB 7 and GMB 11 had similarity on medium catechine and the highest yield, and GMB 3 and GMB 8 had similarity on low catechine. PS 1 with the lowest catechine gallo was separated to the others. The sixth cluster were SA 40, Kiara 8, SA 35, RB 3, and Cin 143 were characterized by low catechine and yields.Â

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Hubungan Kekerabatan Genetik Antar Tanaman The F1 dari Persilangan TRI 2024 X PS 1 Berdasarkan Penanda RAPD

Cited by 3 publications

References 0 publications

Genetic Variability of 21 Tea Genotypes [Camellia sinensis (L.) O. Kuntze] Based on RAPD Markers

Genetic Variability of 21 Tea Genotypes [Camellia sinensis (L.) O. Kuntze] Based on RAPD Markers

Analysis of leaves morphology similarity of several first generation tea clones

An assessment of genetic relationship in tea clones based on important chemical compound and yield potential

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