HUBUNGAN ANTARA MUSIM DENGAN KADAR CAULERPIN DALAM Caulerpa racemosa

Januar, Hedi Lndra; Wikanta, Thamrin; Hastarini, Emma

doi:10.15578/jppi.10.3.2004.1-6

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hal ini dapat dikarenakan sumber tanaman yang digunakan pada penelitian ini dipanen pada tahun dan musim yang berbeda. Musim akan dengan perbedaan keadaan lingkungan seperti, nilai pH (Power of hydrogen), intensitas matahari, jumlah dan kualitas dari nutrisi yang diserap oleh tumbuhan akan menyebabkan perbedaan jumlah dan kualitas metabolit sekunder yang dihasilkan akan berbeda pula [26][27][28] .…”

Section: Metode Penelitianunclassified

Uji antioksidan dan bioaktivitas fraksi etil asetat batang jarak tintir (Jatropha multifia L.)

Syam

Ratnawati

Hermawati

et al. 2020

RIK

View full text Add to dashboard Cite

Abstrak Latar Belakang: Jatropha multifida L. atau jarak tintir merupakan tanaman yang digunakan secara empiris getahnya sebagai obat luka oleh masyarakat Indonesia sejak lama. Penelitian ini merupakan lanjutan dari penelitian sebelumnya mengenai batang jarak tintir yang telah diuji toksisitas dengan metode BSLT dan peredaman antioksidan dengan metode DPPH yang secara berturut memberikan nilai LC50 terendah 3,69 µg/mL dan IC50 56,85 µg/mL pada ekstrak etil asetat. Metode: Penelitian meliputi tahap-tahap pengumpulan bahan uji batang jarak tintir, penyiapan simplisia, pembuatan ekstrak dengan 3 pelarut yang berbeda kepolarannya penapisan fitokimia dari simplisia, fraksinasi menggunakan metode Kromatografi Cair Vakum (KCV) dari ekstrak etil asetat menjadi 4 fraksi. Hasil: Hasil pengujian antioksidan metode peredaman DPPH memberikan IC50 terhadap f1 (n-heksan 75%:etil asetat 25%), f2 (n-heksan 50%:etil asetat 50%), f3 (n-heksan 25%:etil asetat 75%), dan f4 (etil asetat 100%) secara berturut-turut adalah 3759,79 µg/mL; 790,94 µg/mL; 167,41 µg/mL; dan 164,82 µg/mL. Hasil pengujian BSLT terhadap fraksi etil asetat memberikan hasil LC50 sebesar f1 64,43 µg/mL; f2 37,54 µg/mL; f3 41,94 µg/mL; dan f4 25,70 µg/mL. Kesimpulan: F4 merupakan fraksi dengan nilai IC50 dan LC50 yang paling rendah dari seluruh fraksi, yang menunjukkan f4 merupakan fraksi yang paling aktif sebagai antioksidan dan BSLT serta diduga golongan flavonoid yang diketahui dari profil kromatogram KLT dengan Rf 0,57 yang memberikan hasil positif terhadap penampak bercak FeCl3 dan AlCl3 merupakan senyawa aktif dari kedua aktifitas tersebut. Kata kunci: Antioksidan, Brine Shrimp Lethality Test, DPPH, Jatropha multifida L. ANTIOXIDANT AND BIOACTIVITY ASSAYS OF ETHYL ACETATE FRACTION OF JARAK TINTIR STEM (Jatropha multifida L) Abstract Background: Jatropha multifida L. or jarak tintir was empirically used as wound treatment for the sap by Indonesian people for a long time ago. The toxicity and antioxidant capacity of the jarak tintir stem was done by brain shrimp lethality test (BSLT) with LC50 3,69 µg/mL and DPPH scavenging activity with IC50 56,85 µg/mL for ethyl acetate extract. Method: Extraction was performed by continuous extraction with Soxhlet apparatus using various polarity solvents (n-hexane, ethyl acetate, and methanol) and ethyl acetate extract was fractioned by vacuum liquid chromatography (VLC) into 4 fraction with gradien eluen of n-hexane and ethyl acetate. Results: Antioxidant assay gave IC50 for f1 (n-hexane 75%:ethyl acetate 25%), f2 (n-hexane 50%: ethyl acetate 50%), f3 (n-hexane 25%: ethyl acetate 75%), and f4 (ethyl acetate 100%) respectively as 3759,79 µg/mL; 790,94 µg/mL; 167,41 µg/mL; and 164,82 µg/mL. BSLT assays for each fractions gave LC50 as f1 64,43 µg/mL; f2 37,54 µg/mL; f3 41,94 µg/mL; and f4 25,70 µg/mL. Conclusion: Fraction 4 was the best fraction with the lowest IC50 and LC50 with flavonoid was suspected as responsible compound for both assays after TLC analysis that gave spot with Rf 0.57 and gave positive result after sprayed with FeCl3 dan AlCl3 spray reagent. Keywords: Antioxidant, Brine Shrimp Lethality Test, DPPH, Jatropha multifida L

show abstract

Section: Metode Penelitianunclassified

Uji antioksidan dan bioaktivitas fraksi etil asetat batang jarak tintir (Jatropha multifia L.)

Syam

Ratnawati

Hermawati

et al. 2020

RIK

View full text Add to dashboard Cite

show abstract

“…Some studies on edible Caulerpa from the Binuangeun coast have been reported previously, i.e. metabolite (Januar, Wikanta, & Hastarini, 2004;Sihono, Tarman, Madduppa, & Januar, 2018) and nutrition profiles (Putri, Jusadi, Setiawati, & Sunarno, 2017;Suryaningrum & Samsudin, 2019) as well as antioxidant activities (Nursid et al, 2016;Sihono et al, 2018). However, studies on fatty acids, amino acids, heavy metals, and molecular identification of these seaweeds have never been reported yet.…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification, Nutritional Profile and Heavy Metals Content of Edible Caulerpa from Binuangeun Coast, Banten

Sihono

Utomo

Nurhayati

2021

Squalen Bull. Marine Fisheries Postharvest Biotech.

View full text Add to dashboard Cite

Two species of Caulerpa, locally known as “Pedesan” and “Latuh” have been traditionally consumed by coastal communities at Binuangeun, Banten. This study aimed to identify “Pedesan” and “Latuh” using the DNA barcoding method and to evaluate their nutrient and heavy metal contents. Fatty acids were determined by Gas Chromatography Flame Ionization Detector (GC FID), amino acids using Ultra Performance Liquid Chromatography (UPLC), and minerals using Inductively Coupled Plasma Optical Emission Spectrometry (ICP OES). Based on the tufA gene sequences, the “Pedesan” was identified as Caulerpa racemosa var. macrophysa and “Latuh” as Caulerpa chemnitzia. Thirteen fatty acids were detected in C. racemosa var. macrophysa and twelve fatty acids in C. chemnitzia. Of the total fatty acid content, C. racemosa var. macrophysa contained 41.0% unsaturated fatty acids, dominated by linolenic acid and eicosapentaenoic acid. Meanwhile, C. chemnitzia contained 47.5%, dominated by oleic acid. Both seaweeds contained fatty acids with the w6/w3 ratio lower than 10, which could prevent heart disease risk based on World Health Organization (WHO) recommendation. The primary amino acids content in C. racemosa var. macrophysa were glutamic acid, alanine, serine and aspartic acid, while those in C. chemnitzia were glutamic acid, serine, aspartic acid, and glycine. The high content of glutamic acid in both samples indicated their potential use as food flavor enhancer. The Na/K ratio of C. racemosa var. macrophysa (40.31) and C. chemnitzia (27.48) were higher than those recommended by WHO. Heavy metals were not detected in either “Pedesan” nor “Latuh”, indicating that they are safe for consumption.

show abstract

HUBUNGAN ANTARA MUSIM DENGAN KADAR CAULERPIN DALAM Caulerpa racemosa

Abstract: Makroalga hijau caulerpa racemosa dipanen pada musim berbeda, yaitu pada musim penghujan (Mei 2003) dan pada musim kemarau (Agustus 2003).

Cited by 2 publications

References 0 publications

Uji antioksidan dan bioaktivitas fraksi etil asetat batang jarak tintir (Jatropha multifia L.)

Uji antioksidan dan bioaktivitas fraksi etil asetat batang jarak tintir (Jatropha multifia L.)

Molecular Identification, Nutritional Profile and Heavy Metals Content of Edible Caulerpa from Binuangeun Coast, Banten

Contact Info

Product

Resources

About