HubP, a Polar Landmark Protein, Regulates Flagellar Number by Assisting in the Proper Polar Localization of FlhG in Vibrio alginolyticus

Abstract

The marine bacterium

Localization and domain characterization of the SflA regulator of flagellar formation in Vibrio alginolyticus

“…We previously showed that mutation in hubP resulted in an increase in the number of polar flagella (Takekawa et al . ). This phenotype was similar to that of the flhG mutant.…”

“…Recently, we showed that the Δ hubP mutant exhibited a multipolar flagellar phenotype similar to that of the flhG mutant, although the flagella were fewer in number than in the flhG mutant (Takekawa et al . ). In this study, we investigated the subcellular localization of SflA and the functional importance of its C‐terminal cytoplasmic region (SflA C ).…”

“…Recently, we showed that HubP strongly affected the number of flagella, and proposed a novel model with HubP involved in the regulation of flagellar number, in addition to FlhF and FlhG, in V. alginolyticus (Takekawa et al . ). Here, we showed that SflA might interact with HubP directly or indirectly, and HubP anchored SflA at a polar position in cells.…”

“…The hubP deletion mutants were generated from the LPN2 (Δ flhFG ) and LPN4 (Δ flhFG Δ sflA ) strains by homologous recombination with the Δ hubP sequence as previously described (Takekawa et al . ) to generate NMB316 and NMB317. V. alginolyticus cells were grown with shaking at 30 °C in VC medium (0.5% [wt/vol] polypeptone, 0.5% [wt/vol] yeast extract, 0.4% [wt/vol] K 2 HPO 4 , 3.0% [wt/vol] NaCl, 0.2% [wt/vol] glucose) or VPG medium (1.0% [wt/vol] polypeptone, 0.4% [wt/vol] K 2 HPO 4 , 3.0% [wt/vol] NaCl, 0.5% [wt/vol] glycerol).…”

“…; Takekawa et al . ). Finally, we discussed how FlhF, FlhG, SflA, and HubP contribute for the spatial and numerical regulation of polar flagellum in Vibrio species.…”