Huang-Lian-Jie-Du-Decoction Ameliorates Hyperglycemia and Insulin Resistant in Association With Gut Microbiota Modulation

Chen, Mingyi; Liao, Ziqiong; Lu, Biyu; Wang, Mengxia; Lei, Lin; Zhang, Shaobao; Li, Yuan; Liu, Deliang; Liao, Qiongfeng; Xie, Zenghong

doi:10.3389/fmicb.2018.02380

Cited by 90 publications

(60 citation statements)

References 71 publications

(76 reference statements)

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“…On the other hand, the three HFD groups had lower levels of some SCFAs‐producing bacteria, such as Blautia , Butyrivibrio , and Ruminococcaceae_UCG‐010 . In addition, Akkermansia , which is known as maintaining the integrity of intestinal mucosal function, was decreased in the three HFD groups, and this was consistent with Akkemansia enriched in the ZT12CON by LEfSe analysis. Therefore, either at ZT0 or at ZT12, the intake of FO or AO selectively enhanced the populations of several beneficial genera.…”

Section: Discussionsupporting

confidence: 76%

ω‐3 PUFAs Alleviate High‐Fat Diet–Induced Circadian Intestinal Microbes Dysbiosis

Gui

Chen

Wang

et al. 2019

Molecular Nutrition Food Res

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Scope Published data support that fish oil and algae oil rich in ω‐3 polyunsaturated fatty acids (PUFAs) protect against hyperlipidemia in mice. This study is aimed to explore the effects of fish oil and algae oil on high‐fat diet (HFD) induced circadian intestinal microbes dysregulation. Methods and Results Male C57BL/6 mice are randomly divided into four groups, which are fed a normal chow diet (CON), a HFD, a HFD supplemented with fish oil (FO), and a HFD supplemented with algae oil (AO), respectively, for 12 weeks. At the end of the experiment, mice are sacrificed at 12 h intervals with the first one at zeitgeber time 0 (ZT0) and the second at zeitgeber time 12 (ZT12). FO and AO groups ameliorate diet‐induced hyperlipidemia. The relative abundance of certain genera is improved in FO and AO groups according to 16S rRNA gene sequencing. The short‐chain fatty acids (SCFAs) producing bacteria Butyricimonas and some of the genera in the Lachnospiraceae recover to the normal circadian rhythm in both FO and AO groups. Conclusion The data show that FO and AO alleviate circadian gut microbiota dysregulation in mice caused by HFD, and support the further investigation of ω‐3 PUFAs as a dietary intervention strategy for the prevention of hyperlipidemia.

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Section: Discussionsupporting

confidence: 76%

ω‐3 PUFAs Alleviate High‐Fat Diet–Induced Circadian Intestinal Microbes Dysbiosis

Gui

Chen

Wang

et al. 2019

Molecular Nutrition Food Res

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“…Component crude drugs of BTS, Atractylodis Rhizoma and Rhei Rhizoma, which are reported to augment the genus Akkermansia in the gut [51,52], are known to include bioactive glycosides atractylodis and sennoside, respectively. Chen et al reported that the genus Akkermansia was increased by administration of another Kampo drug, orengedokuto, in a high-fat diet and streptozotocin induced type 2 diabetic model rat [53]. Gardeniae Fructus and Scutellariae Radix are common crude drugs in both orengedokuto and BTS.…”

Section: Discussionmentioning

confidence: 99%

Increase of Akkermansia muciniphila by a Diet Containing Japanese Traditional Medicine Bofutsushosan in a Mouse Model of Non-Alcoholic Fatty Liver Disease

et al. 2020

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Non-alcoholic fatty liver disease (NAFLD) is considered a worldwide healthcare problem that mirrors the increased prevalence of obesity. Gut microbiota plays a crucial role in the progression and treatment of NAFLD. Bofutsushosan (BTS), a pharmaceutical-grade Japanese traditional medicine, has long been prescribed in Japan for obesity and obesity-related syndrome. Although BTS has been reported to exert an anti-obesity effect in obese patients as well as various obesity-model animals, its effect on gut microbiota is unknown. Here, the effects of BTS on obesity, liver damage, and the gut microbiome in genetically obese mice, ob/ob, were studied. Seven-week-old ob/ob mice were fed a standard diet with (BTS group) or without (CONT group) 5% BTS for 4 weeks. By comparison to the CONT group, the BTS group showed reduced body weight gain and hyperlipidemia as well as improved liver function. Moreover, gut microbiota in the CONT and BTS group formed a significantly different cluster. Specifically, the genera Akkermansia, Bacteroides and an unknown genus of the family Enterobacteriaceae expanded dramatically in the BTS group. Noteworthy, the population of Akkermansia muciniphila, which is reported to elicit an anti-obesity effect and improve various metabolic abnormalities, was markedly increased (93-fold) compared with the CONT group. These results imply that BTS may be a promising agent for treating NAFLD.

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“…AF12 sp. is an under-characterized microbe, with some literature nding a correlation between higher abundance and lower body weight, although some evidence is con icting [100][101][102][103]. Additionally, AF12 sp.…”

Section: Discussionmentioning

confidence: 99%

Understanding the Physiological Functions of the Host Xenobiotic-sensing Nuclear Receptors PXR and CAR on the Gut Microbiome using Genetically Modified Mice

Little

Dutta

et al. 2020

Preprint

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Background: Pharmacological activation of the host xenobiotic-sensing nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) is well-known to increase drug metabolism and reduce inflammation. Little is known regarding the physiological functions of PXR and CAR on the gut microbiome, which is an important regulator for the host immune surveillance and bile acid (BA) metabolism. We examined the gut microbiome composition and BA metabolites in high vs. low PXR/CAR-expressing mice, and in mice that are deficient in PXR, CAR, or both, at two developmental ages. We also utilized humanized PXR transgenic (hPXR-TG) mice to compare the species-specific effect of PXR on the gut microbiome. Results: We discovered bivalent hormetic functions of PXR and CAR in modulating the richness of the gut microbiome and inflammatory biomarkers: the high PXR/CAR expressers had higher microbial richness, pro-inflammatory bacteria (distinct taxa in Helicobacteraceae and Helicobacter), and fecal pro-inflammatory cytokines, suggesting higher immune surveillance to prevent the colonization of harmful bacteria. Interestingly, the absence of PXR or CAR also increased the microbial richness, and absence of both receptors synergistically increased the microbial richness. PXR and CAR deficiency increased the pro-inflammatory bacteria (Helicobacteraceae and Helicobacter). Most notably, deficiency in both PXR and CAR markedly increased the relative abundance of Lactobacillus, which is capable of bile salt hydrolase (BSH) activity. This corresponded to a decrease in major primary taurine-conjugated bile acids (BAs) in feces, which may lead to higher internal burden of taurine and unconjugated BAs, both of which are linked to inflammation, oxidative stress, and cytotoxicity. hPXR-TG mice had a distinct microbial profile as compared to wild-type mice, including a higher representation of Prevotella. hPXR-TG mice also had higher 12-OH BAs but lower 6-OH BAs, suggesting PXR’s species-specific role in modulating host hepatic BA synthesis. Conclusions: This study is the first to show that the host PXR and CAR profoundly influence the composition of the gut microbiome and its BA metabolites, with a bivalent hormetic relationship between PXR/CAR levels and microbial richness, unveiling the involvement of PXR/CAR-microbiome interactions in host immune surveillance and BA metabolism.

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Huang-Lian-Jie-Du-Decoction Ameliorates Hyperglycemia and Insulin Resistant in Association With Gut Microbiota Modulation

Cited by 90 publications

References 71 publications

ω‐3 PUFAs Alleviate High‐Fat Diet–Induced Circadian Intestinal Microbes Dysbiosis

ω‐3 PUFAs Alleviate High‐Fat Diet–Induced Circadian Intestinal Microbes Dysbiosis

Increase of Akkermansia muciniphila by a Diet Containing Japanese Traditional Medicine Bofutsushosan in a Mouse Model of Non-Alcoholic Fatty Liver Disease

Understanding the Physiological Functions of the Host Xenobiotic-sensing Nuclear Receptors PXR and CAR on the Gut Microbiome using Genetically Modified Mice

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