Huai Qi Huang corrects the balance of Th1/Th2 and Treg/Th17 in an ovalbumin-induced asthma mouse model

Peng, Liang; Shao, Pengfei; Zhang, Man; Ma, Yingying; Zhen, Xinggang; Li, Huijuan

doi:10.1042/bsr20171071

Cited by 51 publications

(42 citation statements)

References 23 publications

(27 reference statements)

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“…Type 2 helper T ( 2) cells seem to play a pivotal role in immune dysfunction, which contributes to the development of asthma [5][6][7]. In addition, it has been observed that the 2-associated cytokines (e.g., interleukin-4 (IL-4), IL-5, and IL-13) were released by the airway epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

Effects of Pelargonium sidoides and Coptis Rhizoma 2 : 1 Mixed Formula (PS + CR) on Ovalbumin-Induced Asthma in Mice

Min

Park

Choi

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Pelargonium sidoides (PS) is traditionally used to treat respiratory and gastrointestinal infections, dysmenorrhea, and hepatic disorders in South Africa. Coptis Rhizoma (CR) is used to treat gastroenteric disorders, cardiovascular diseases, and cancer in East Asia. In the present study, we intended to observe the possible beneficial antiasthma effects of PS and CR on the ovalbumin- (OVA-) induced asthma C57BL/6J mice. Asthma in mice was induced by OVA sensitization and subsequent boosting. PS + CR (300 and 1,000 mg/kg; PO) or dexamethasone (IP) was administered once a day for 16 days. The changes in the body weight and gains, lung weights and gross inspections, total and differential cell counts of leukocytes in bronchoalveolar lavage fluid (BALF), serum OVA-specific immunoglobulin E (OVA-sIgE) levels, interleukin-4 (IL-4) and IL-5 levels in BALF and lung tissue homogenate, and IL-4 and IL-5 mRNA levels in lung tissue homogenates were analyzed with lung histopathology: mean alveolar surface area (ASA), alveolar septal thickness, numbers of inflammatory cells, mast cells, and eosinophils infiltrated in the alveolar regions, respectively. Significant increases in lung weights, total and differential cell counts of leukocytes in BALF, serum OVA-sIgE levels, and IL-4 and IL-5 levels in BALF and lung tissue homogenate were observed in OVA control as compared to those of intact control. In addition, OVA control showed a significant decrease in mean ASA and increases in alveolar septal thickness, numbers of inflammatory cells, mast cells, and eosinophils infiltrated in alveolar regions. However, these allergic and inflammatory asthmatic changes were significantly inhibited by PS + CR in a dose-dependent manner. In this study, PS + CR showed dose-dependent beneficial effects on OVA-induced asthma in mice through anti-inflammatory and antiallergic activities. Therefore, it is expected that PS + CR have enough potential as a new therapeutic agent or as an ingredient of a medicinal agent for various allergic and inflammatory respiratory diseases including asthma.

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Section: Introductionmentioning

confidence: 99%

Effects of Pelargonium sidoides and Coptis Rhizoma 2 : 1 Mixed Formula (PS + CR) on Ovalbumin-Induced Asthma in Mice

Min

Park

Choi

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…The above evidence proves that the imbalance of Th1/Th2 and Th17/Treg, to some degree, contributes to kidney diseases [19]. In the experiment of mice, Liang et al found that HQH could regulate Th1/Th2 and Treg/Th17 via the rebalance of cytokine profiles and change the mRNA expression levels of the transcription factors [16]. The levels of IFN-γ in plasma were positively correlated with the dosage of HQH [20].…”

Section: Mechanismmentioning

confidence: 95%

“…The characterization of CD4+ T-cells reflects the immune status and is important in the maintenance of tumorigenesis and homeostasis. HQH markedly increased the classic Th1 cytokine IFN-γ and the Treg cytokine IL-10 and TGF-β but decreased the Th2 cytokines IL-4, IL-5, and IL-13 and the Th17 cytokine IL-17, indicating that it regulates both the Th1/Th2 balance and the Th17/Treg balance [16]. Li et al found that patients with renal cell carcinoma (RCC) are observed to have a skewed in Th1/Th2 and Th17/Treg balance [17].…”

Section: Mechanismmentioning

confidence: 97%

“…Primary Nephrotic Syndrome. Primary nephrotic syndrome (PNS) is a frequently occurring disease in the urinary system in childhood with the acceleration of modernization and urbanization, which is characterized by massive proteinuria, hypoproteinemia, hyperlipidemia, and edema [16][17][18]. It is caused by increased permeability of glomerular filtration membrane to plasma protein and loss of large amounts of plasma albumin from urine.…”

Section: Mesangioproliferative Glomerulonephritis (Mspgn)mentioning

confidence: 99%

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The Effect of Chinese Traditional Medicine Huaiqihuang (HQH) on the Protection of Nephropathy

Zhang

Cheng

Zhou

et al. 2020

Oxidative Medicine and Cellular Longevity

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Kidney disease is one of the common diseases with high morbidity and high mortality, which brings a huge burden to the society and the patient’s family. The pathogenesis, treatment, and prognosis of kidney diseases are related to oxidative stress, inflammation, mitochondrial damage, and immune dysfunction. However, existing treatments always cause some damage to the kidneys. Kidney disease and immunosuppressant used together often lead to drug toxicity, patients with weakened immunity, organic rupture of the normal structure of the kidney, damage to the physiological function of the kidney, etc. Huaiqihuang is a kind of traditional Chinese medicine with a history of more than one thousand years. According to research, Robinia pseudoacacia can regulate the immune function by regulating oxidative stress, calcium inflow, and mitochondrial ATP. At the same time, it is also involved in regulating the ways of cell death, such as apoptosis, autophagy, ferroptosis, pyroptosis, and clockophagy, to reduce kidney damage, which has important clinical value. This article reviews the exact mechanism and clinical application of Huaiqihuang in different types of nephropathy. The aim is to provide new ideas for the treatment of clinical nephropathy.

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“…In the Anti-PD-1 group, mice were injected intraperitoneally with anti-PD-1 (200 μg/mouse; clone RMP1–14) each day from day 18 to 20 consecutively. The left lobe of the lung was used to prepare a single-cell suspension for flow cytometry for Treg and Th17 cells as described previously [32]. …”

Section: Methodsmentioning

confidence: 99%

Correlation of PD-1/PD-L1 Signaling Pathway with Treg/Th17 Imbalance from Asthmatic Children

Xia

Liu

Guo

2018

Int Arch Allergy Immunol

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Background: The balance between T helper 17 (Th17) and regulatory T cells (Treg) is a new paradigm in asthma pathogenesis, but no therapeutic targets could modulate the Th17/Treg balance specifically for asthma. Since previous studies have shown the programmed cell death-1(PD-1)/PD-ligand 1 (PD-L1) pathway is critical to immune homeostasis in this disease, we hypothesized that the PD-1/PD-L1 pathway might be involved in the regulation of Treg/Th17 imbalance in asthmatic children. Methods: The percentage of Treg and Th17 cells and the expression of PD-1 and PD-L1 were detected by flow cytometry in children with asthma and healthy controls. CD4⁺ T cells were stimulated with Th17 and Treg differentiating factors, and treated with anti-PD-1. Then cells were harvested and measured for Th17 and Treg percentages and Foxp3 and RORγt levels using RT-PCR. Results: We observed an inverse correlation between the percentages of Treg and Th17 cells, and the expression of PD-1 and PD-L1 in the two subsets also changed in the mild persistent and moderate to severe persistent groups compared with healthy controls. In vitro, administration of anti-PD-1 could decrease Th17 percentages and RORγt mRNA, and increase Treg percentages and Foxp3 mRNA in CD4⁺ T cells of children with asthma in the mild persistent and moderate to persistent groups. Additionally, the role played by anti-PD-1 in regulating Treg/Th17 balance was further confirmed in an asthmatic mouse model. Conclusion: Alteration of the PD-1/PD-L1 pathway can modulate Treg/Th17 balance in asthmatic children. Treatment with anti-PD-1 posed protective effects on asthma models, providing a novel theoretical target for asthma.

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Huai Qi Huang corrects the balance of Th1/Th2 and Treg/Th17 in an ovalbumin-induced asthma mouse model

Cited by 51 publications

References 23 publications

Effects of Pelargonium sidoides and Coptis Rhizoma 2 : 1 Mixed Formula (PS + CR) on Ovalbumin-Induced Asthma in Mice

Effects of Pelargonium sidoides and Coptis Rhizoma 2 : 1 Mixed Formula (PS + CR) on Ovalbumin-Induced Asthma in Mice

The Effect of Chinese Traditional Medicine Huaiqihuang (HQH) on the Protection of Nephropathy

Correlation of PD-1/PD-L1 Signaling Pathway with Treg/Th17 Imbalance from Asthmatic Children

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