2001
DOI: 10.1074/jbc.m109511200
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HuA and Tristetraprolin Are Induced following T Cell Activation and Display Distinct but Overlapping RNA Binding Specificities

Abstract: AU-rich elements found in the 3-untranslated regions of cytokine and proto-oncogene transcripts regulate mRNA degradation and function as binding sites for the mRNA-stabilizing protein HuA and the mRNA-destabilizing protein tristetraprolin. Experiments were performed to evaluate the expression of HuA and tristetraprolin in purified human T lymphocytes and to evaluate the ability of these proteins to recognize specific AU-rich sequences. HuA is a predominantly nuclear protein that can also be found in the cytop… Show more

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Cited by 127 publications
(136 citation statements)
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References 45 publications
(67 reference statements)
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“…Proximity of HuR-and TIS11b-binding elements in VEGF 3 0 UTR suggests that stabilizing and destabilizing AREbinding proteins may compete for binding to similar regions of the 3 0 UTR as it has been recently observed for several cytokine mRNAs (Raghavan et al, 2001). Interestingly, this TBE sequence has previously been shown to be involved in the stabilization of VEGF mRNA after JNK and p38/HOG activation (Pages et al, 2000).…”
Section: Discussionmentioning
confidence: 98%
“…Proximity of HuR-and TIS11b-binding elements in VEGF 3 0 UTR suggests that stabilizing and destabilizing AREbinding proteins may compete for binding to similar regions of the 3 0 UTR as it has been recently observed for several cytokine mRNAs (Raghavan et al, 2001). Interestingly, this TBE sequence has previously been shown to be involved in the stabilization of VEGF mRNA after JNK and p38/HOG activation (Pages et al, 2000).…”
Section: Discussionmentioning
confidence: 98%
“…However, the JNK response element maps to the 5Ј end of IL-2 mRNA, so JNK may not have a direct effect on ARE-binding proteins (14,25). T cell activation leads to an increase in expression of tristetraprolin and HuR (26), but HuR does not recognize the specific AU-rich region in the IL-2 mRNA and another AU-binding protein, NF90, has been implicated in signal-dependent IL-2 mRNA stabilization (27). JNK has been implicated as a potential site for signal integration between TCR and CD28 (28), but JNK1/JNK2 double KOs have little defect in T cell activation (29).…”
Section: Discussionmentioning
confidence: 99%
“…Additional studies have shown that mutants not binding TNFa or GM-CSF AREs may inhibit the RNA turnover of these mRNAs, eventually failing to recruit the poly(A)-binding proteins. In activated T lymphocytes, TTP was associated with HuR in a homeostatic complex that transiently regulates the cytoplasmic level of relevant RNAs (Raghavan et al, 2001).…”
Section: Tristetraprolinmentioning
confidence: 99%