HSP27 is markedly induced in Schwann cell columns and associated regenerating axons

Hirata, Ken‐ichi; He, J.; Hirakawa, Yasuyuki; Liu, Wenting; Wang, Songyan; Kawabuchi, Masaru

doi:10.1002/glia.10105

Cited by 37 publications

(22 citation statements)

References 45 publications

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“…Induction of HSP27 has been observed in Schwann cells and in the regenerating axons after nerve injury [26]. In addition, HSP27 is also constitutively produced in sensory and motor neurons, as well as in the surrounding satellite cells [27].…”

Section: Neuronal Aspects Of Hsp27mentioning

confidence: 93%

Heat shock protein 27 is associated with better nerve function and fewer signs of neuropathy

et al. 2011

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Aims/hypothesis High levels of serum heat shock protein 27 (sHSP27) have been associated with distal symmetric polyneuropathy in patients with type 1 diabetes. Our objective was to investigate the association between sHSP27, neuropathic signs and nerve function in individuals with normal glucose tolerance (NGT), impaired glucose tolerance (IGT) and type 2 diabetes. Methods Participants were recruited consecutively from the population-based Västerbotten Intervention Program (NGT, n=39, IGT, n=29, and type 2 diabetes, n=51) and were matched for age and sex. sHSP27 levels were measured and nerve conduction studies were performed (peroneal and sural nerves). z Scores for each nerve conduction measure were calculated and compiled into a composite z score for the leg. Neuropathy disability score (NDS) was used to assess neuropathic signs. Results Patients with diabetes had significantly lower sHSP27 levels (geometric mean sHSP27 206 pg/ml, 95% CI 142, 299) than those with IGT (geometric mean sHSP27 455 pg/ml, 95% CI 319, 650, p<0.05) and controls (geometric mean sHSP27 361 pg/ml, 95% CI 282, 461, p<0.05). Participants with few signs of neuropathy (first tertile, NDS ≤2) had significantly higher sHSP27 levels (geometric mean sHSP27 401 pg/ml, 95% CI 310, 520) than participants with many signs (third tertile, NDS ≥7) (geometric mean sHSP27 192 pg/ml, 95% CI 128, 288, p=0.007). The highest sHSP27 tertile was associated with better nerve function, adjusted for age, sex, statin medication and HbA 1c (OR 2.51, 95% CI 1.25, 5.05, p<0.05). Conclusions/interpretation High sHSP27 levels were associated with better nerve function and fewer neuropathic signs in NGT, IGT and type 2 diabetes.

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Section: Neuronal Aspects Of Hsp27mentioning

confidence: 93%

Heat shock protein 27 is associated with better nerve function and fewer signs of neuropathy

et al. 2011

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“…The fluorescent immunohistochemical procedure was as described previously (Hirata et al 2000(Hirata et al , 2003.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Expression of TGF-?-like molecules in the life cycle of Schistosoma japonicum

Hirata

Hara

et al. 2005

Parasitol Res

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The transforming growth factor beta (TGF-beta) family controls an extremely wide range of biological activities, such as the growth and differentiation of cells, and immunological events against infectious agents. Although TGF-beta homologs appear to be widely present in metazoan animals, studies of parasite-derived molecules are relatively few. Using antibodies against anti-mouse TGF-beta1, -beta2, and -beta3, we show the expression of TGF-beta-like molecules in Schistosoma japonicum cercariae, schistosomula, eggs and adult worms. Intense immunoreactivity was found on the surface of free-living cercarial bodies. In transverse sections of cercariae, the molecules were localized in the tegument and subtegumental cells, and the number and distribution of producing cells significantly differed with each antibody. In the skin-migrating stage, the expression in the tegumental surface gradually decreased and became almost negative within 48 h of exposure. In adult worms and eggs, the reactivity was found in subtegumental cells and in cells of a tubular structure, respectively. In western blot analysis, the detection of conventional TGF-beta molecules failed. The expression of TGF-beta-like molecules was distinctly regulated at each developmental stage.

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“…55 Another mechanism may be through the upregulation of heat-shock proteins, which can increase the rate of survival of motor 56 and sensory 57 neurons after injury and induce the process of nerve regeneration. 58,59 However, although thyroid hormone has been shown to induce the expression and phosphorylation of heat-shock protein 27 in heart tissues, 60 it remains largely unknown whether similar effect can occur in nerve tissue. This issue merits further investigation.…”

Section: Discussionmentioning

confidence: 99%

Functional effects of local thyroid hormone administration after sciatic nerve injury in rats

Papakostas

Mourouzis

et al. 2008

Microsurgery

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This study investigates potential functional effects of thyroid hormone (T3) on peripheral nerve regeneration in rats. Forty adult male Lewis rats were included in this study. After complete transection of the right sciatic nerve, the gap between the stumps was bridged with a silicone tube. In the first experimental group (group A, n = 12), T3 solution was used to fill the tube, whereas a sterile buffer solution was used in nontreated rats, (group B, n = 12). Additionally, sham operation with surgical incision and mobilization of the sciatic nerve without any other intervention was performed (group C, n = 10). In a few animals, alpha-segment of the nerve was excised and the stumps were reversed to exclude the possibility of regeneration (group D, n = 6). The process of peripheral nerve regeneration was assessed by functional indices at 3, 6, 9, 13, and 17 weeks postoperatively. Mid-stance angle, at the ankle, measured in degrees, was used as a kinematic index and the withdrawal reflex (measured in grams of applied force) was used to evaluate the return of sensory function. Kinematic indexes were not different between the groups A and B at all time points of the evaluation. Sensory function was significantly different in T3-treated animals compared with buffer-treated control group (x vs. y, P = 0.031) at 9 weeks. Thereafter, sensory function was comparable between groups. In conclusion, T3 seems to accelerate the return of sensory function after complete transection of the sciatic nerves in Lewis rats without a significant effect on motor nerve recovery.

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HSP27 is markedly induced in Schwann cell columns and associated regenerating axons

Cited by 37 publications

References 45 publications

Heat shock protein 27 is associated with better nerve function and fewer signs of neuropathy

Heat shock protein 27 is associated with better nerve function and fewer signs of neuropathy

Expression of TGF-?-like molecules in the life cycle of Schistosoma japonicum

Functional effects of local thyroid hormone administration after sciatic nerve injury in rats

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