Hsa_circ_0123190 acts as a competitive endogenous RNA to regulate APLNR expression by sponging hsa-miR-483-3p in lupus nephritis

Zhang, Chunyi; Gao, Congcong; Di, Xing; Cui, Siwan; Liang, Wenfang; Sun, Wenbo; Yao, Meng; Liu, Shengyun; Zheng, Zhaohui

doi:10.1186/s13075-020-02404-8

Cited by 21 publications

(15 citation statements)

References 30 publications

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“…A large amount of evidence has shown that circRNAs regulated the pathological processes of renal diseases [ 20 ]. For example, circSDHC contributed to cell invasion and proliferation in renal cell carcinoma [ 21 ]; circ-Ttc3 promoted the inflammatory response and oxidative stress in sepsis-induced acute kidney injury [ 22 ]; hsa_circ_0123190 was significantly downregulated in lupus nephritis and it might impede the progression of lupus nephritis [ 23 ]. Circ-ACTR2 has been upregulated in DN patients and HG-treated renal tubular cells [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

Circ-ACTR2 aggravates the high glucose-induced cell dysfunction of human renal mesangial cells through mediating the miR-205-5p/HMGA2 axis in diabetic nephropathy

Yun

Ren

Liu

et al. 2021

Diabetol Metab Syndr

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Background Circular RNAs (circRNAs) have been considered as pivotal biomarkers in Diabetic nephropathy (DN). CircRNA ARP2 actin-related protein 2 homolog (circ-ACTR2) could promote the HG-induced cell injury in DN. However, how circ-ACTR2 acts in DN is still unclear. This study aimed to explore the molecular mechanism of circ-ACTR2 in DN progression, intending to provide support for the diagnostic and therapeutic potentials of circ-ACTR2 in DN. Methods RNA expression analysis was conducted by the quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Cell growth was measured via Cell Counting Kit-8 and EdU assays. Inflammatory response was assessed by Enzyme-linked immunosorbent assay. The protein detection was performed via western blot. Oxidative stress was evaluated by the commercial kits. The molecular interaction was affirmed through dual-luciferase reporter and RNA immunoprecipitation assays. Results Circ-ACTR2 level was upregulated in DN samples and high glucose (HG)-treated human renal mesangial cells (HRMCs). Silencing the circ-ACTR2 expression partly abolished the HG-induced cell proliferation, inflammation and extracellular matrix accumulation and oxidative stress in HRMCs. Circ-ACTR2 was confirmed as a sponge for miR-205-5p. Circ-ACTR2 regulated the effects of HG on HRMCs by targeting miR-205-5p. MiR-205-5p directly targeted high-mobility group AT-hook 2 (HMGA2), and HMGA2 downregulation also protected against cell injury in HG-treated HRMCs. HG-mediated cell dysfunction was repressed by miR-205-5p/HMGA2 axis. Moreover, circ-ACTR2 increased the expression of HMGA2 through the sponge effect on miR-205-5p in HG-treated HRMCs. Conclusion All data have manifested that circ-ACTR2 contributed to the HG-induced DN progression in HRMCs by the mediation of miR-205-5p/HMGA2 axis.

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Section: Discussionmentioning

confidence: 99%

Circ-ACTR2 aggravates the high glucose-induced cell dysfunction of human renal mesangial cells through mediating the miR-205-5p/HMGA2 axis in diabetic nephropathy

Yun

Ren

Liu

et al. 2021

Diabetol Metab Syndr

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“…ROC analysis revealed that circRNA_002453 yielded an AUC value of 0.906 to diagnose LN. Another study identified hsa_circ_0123190 was down-regulated in both renal tissues and peripheral blood of LN patients ( 62 ). There was no significant association between the tissue levels of hsa_circ_0123190 and clinical parameters, while its expression in blood was negatively correlated with serum creatinine, and the AUC-ROC value diagnosing LN was 0.900.…”

Section: Circrnas As Novel Biomarkers For Kidney Diseasesmentioning

confidence: 99%

“…Since miR-150 was previously reported to promote renal fibrosis in LN, these results suggest that circHLA-C might participate in the development of LN by sponging miR-150. The other study found hsa_circ_0123190 was down-regulated in LN and could serve as a sponge for hsa-miR-483-3p, which targets apelin receptor (APLNR) ( 62 ). APLNR has been shown to be involved in renal fibrosis by acting on TGF-β1 and its expression was associated with chronicity index (CI) of LN.…”

Section: Circrnas As Therapeutic Targets For Kidney Diseasesmentioning

confidence: 99%

Circular RNAs as Novel Diagnostic Biomarkers and Therapeutic Targets in Kidney Disease

Xie²,

Huang

et al. 2021

Front. Med.

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Circular RNAs (circRNAs) are a novel type of non-coding RNAs that have aroused growing attention in this decade. They are widely expressed in eukaryotes and generally have high stability owing to their special closed-loop structure. Many circRNAs are abundant, evolutionarily conserved, and exhibit cell-type-specific and tissue-specific expression patterns. Mounting evidence suggests that circRNAs have regulatory potency for gene expression by acting as microRNA sponges, interacting with proteins, regulating transcription, or directly undergoing translation. Dysregulated expression of circRNAs were found in many pathological conditions and contribute to the pathogenesis and progression of various disorders, including renal diseases. Recent studies have revealed that circRNAs may serve as novel reliable biomarkers for the diagnosis and prognosis prediction of multiple kidney diseases, such as renal cell carcinoma (RCC), acute kidney injury (AKI), diabetic kidney disease (DKD), and other glomerular diseases. Furthermore, circRNAs expressed by intrinsic kidney cells are shown to play a substantial role in kidney injury, mostly reported in DKD and RCC. Herein, we review the biogenesis and biological functions of circRNAs, and summarize their roles as promising biomarkers and therapeutic targets in common kidney diseases.

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“…Circular RNA (circRNA), lacking 5′cap and a 3′poly (A) tail, is a stable ring structure with highly conservative sequences across species [ 6 , 7 ], and implicated in the pathophysiology of various renal diseases (e.g., lupus nephritis, diabetic nephropathy, and focal segmental glomerulus sclerosis) [ 8 – 15 ]. Our recent study reported that mmu_circRNA_30032 promoted unilateral ureteral obstruction (UUO)-mice kidney fibrogenesis via microRNA (miRNA) sponge [ 16 ], however, what role the circRNAs exactly played in renal fibrosis caused by mice-UUO and human ON remains largely unknown.…”

Section: Introductionmentioning

confidence: 99%

The mmu_circRNA_37492/hsa_circ_0012138 function as potential ceRNA to attenuate obstructive renal fibrosis

Lee

et al. 2022

Cell Death Dis

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Circular RNAs (circRNAs) are involved in the pathogenesis of certain renal diseases, however, the function and mechanism of them in renal fibrosis remains largely unknown. In the present study, RNA expression data in unilateral ureteral obstruction (UUO) kidneys was obtained from our previous circRNA Microarray and public Gene Expression Omnibus datasets to construct a ceRNA network. The effects of target circRNA as long as the homologous human circRNA on renal fibrosis was examined in vitro and in vivo. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis was further performed among genes regulated by the human circRNA. We found that circRNA_37492, showing well connection degree in the ceRNA network, was abundant expression and high sequence conservation. We observed that the expression of circRNA_37492 was induced by the TGF-β1 or UUO in BUMPT cells and C57BL/6 mice, respectively. In vitro, cytoplasmic circRNA_37492 inhibited type I, III collagen and fibronectin deposition by sponging miR-7682-3p and then upregulated its downstream target Fgb. In vivo, overexpression of circRNA_37492 attenuated fibrotic lesions in the kidneys of UUO mice via targeting miR-7682-3p/Fgb axis. Furthermore, hsa_circ_0012138, homologous with circRNA_37492, may potentially target miR-651-5p/FGB axis in human renal fibrosis. Not only that, GO and KEGG enrichment revealed that hsa_circ_0012138-regulated genes were previously demonstrated to related to the fibrosis. In conclusion, we for the first time demonstrated that circRNA_37492 attenuated renal fibrosis via targeting miR-7682-3p/Fgb axis, and the homologous hsa_circRNA_0012138 was speculated as a possible ceRNA to regulate multiple gene expressions and involve in human renal fibrosis, suggesting that circRNA_37492/hsa_circ_0012138 may serve as potent therapy target for obstructive renal fibrosis disease.

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Hsa_circ_0123190 acts as a competitive endogenous RNA to regulate APLNR expression by sponging hsa-miR-483-3p in lupus nephritis

Cited by 21 publications

References 30 publications

Circ-ACTR2 aggravates the high glucose-induced cell dysfunction of human renal mesangial cells through mediating the miR-205-5p/HMGA2 axis in diabetic nephropathy

Circ-ACTR2 aggravates the high glucose-induced cell dysfunction of human renal mesangial cells through mediating the miR-205-5p/HMGA2 axis in diabetic nephropathy

Circular RNAs as Novel Diagnostic Biomarkers and Therapeutic Targets in Kidney Disease

The mmu_circRNA_37492/hsa_circ_0012138 function as potential ceRNA to attenuate obstructive renal fibrosis

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