Circ-ACTR2 aggravates the high glucose-induced cell dysfunction of human renal mesangial cells through mediating the miR-205-5p/HMGA2 axis in diabetic nephropathy

Yun, Jie; Ren, Jiangong; Liu, Yuanyuan; Dai, Lijuan; Song, Limei; Ma, Xiaopeng; Luo, Shan; Ye-xu, Song

doi:10.1186/s13098-021-00692-x

Cited by 23 publications

(17 citation statements)

References 35 publications

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“…Numerous reports have highlighted circRNAs in the treatment of DN, such as circEIF4G2, hsa_circ_0125310, circTAOK1, and circ-ACTR2 ( Xu et al, 2020 ; Yun et al, 2021 ; Li et al, 2022 ; Zhu et al, 2022 ). A study has indicated that circ-FBXW12 was increased in the serum of DN patients and human MCs by HG-induced.…”

Section: The Role Of Circrnas In Diabetic Complicationsmentioning

confidence: 99%

Circular RNAs in diabetes and its complications: Current knowledge and future prospects

Yin

Zhang²,

Xiao³

et al. 2022

Front. Genet.

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A novel class of non-coding RNA transcripts called circular RNAs (circRNAs) have been the subject of significant recent studies. Accumulating evidence points that circRNAs play an important role in the cellular processes, inflammatory expression, and immune responses through sponging miRNA, binding, or translating in proteins. Studies have found that circRNAs are involved in the physiologic and pathologic processes of diabetes. There has been an increased focus on the relevance of between abnormal circRNA expression and the development and progression of various types of diabetes and diabetes-related diseases. These circRNAs not only serve as promising diagnostic and prognostic molecular biomarkers, but also have important biological roles in islet cells, diabetes, and its complications. In addition, many circRNA signaling pathways have been found to regulate the occurrence and development of diabetes. Here we comprehensively review and discuss recent advances in our understanding of the physiologic function and regulatory mechanisms of circRNAs on pancreatic islet cells, different subtypes in diabetes, and diabetic complications.

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Section: The Role Of Circrnas In Diabetic Complicationsmentioning

confidence: 99%

Circular RNAs in diabetes and its complications: Current knowledge and future prospects

Yin

Zhang²,

Xiao³

et al. 2022

Front. Genet.

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“…Jie et al found that circ-ACTR2 levels were upregulated in diabetic kidney disease and human renal mesangial cells (HRMCs) treated with high glucose. Silencing circ-ACTR2 expression partially abrogated high-glucose-induced cell proliferation, inflammation, ECM accumulation, and oxidative stress in HRMCs [ 17 ]. In addition, miR-205-5p directly targets the high mobility group AT-hook2 (HMGA2), and HMGA2 downregulation protects against high-glucose-treated HRMCs injury.…”

Section: Discussionmentioning

confidence: 99%

Differential expression profile of urinary exosomal microRNAs in patients with mesangial proliferative glomerulonephritis

Dai

Zhang

Jin

et al. 2023

Aging

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Objective: To investigate the differential expression profile of urinary exosomal microRNA (miRNA) in patients with mesangial proliferative glomerulonephritis (MsPGN) and healthy controls and their potential role in the pathogenesis of MsPGN. Methods: Urine specimens were collected from five MsPGN patients and five healthy controls, and differentially expressed miRNAs were screened using high-throughput sequencing technology. The sequenced urinary exosomal miRNAs were further investigated by quantitative real-time polymerase chain reaction (qRT-PCR) in a validation cohort (16 MsPGN patients and 16 healthy controls). Correlation and receiver operating characteristic (ROC) curve analyses were used to determine the association between clinical features and miRNA expression in MsPGN. Finally, fluorescence in situ hybridization was performed to detect miRNA expression in the renal tissues of MsPGN patients. Results: Five differentially expressed miRNAs (miR-125b-2-3p, miR-205-5p, let-7b-3p, miR-1262, and miR-548o-3p) were identified by qRT-PCR. The expression of these miRNAs correlated with ACR, 24hUpro, mAlb, UA, and combined yielded a ROC curve area of 0.916 in discriminating MsPGN patients from the controls. In addition, the expression of miR-205-5p, let-7b-3p, miR-1262, and miR-548o-3p was elevated in the MsPGN patient group, and miR-125b-2-3p was decreased in the MsPGN patient group. Conclusions: Differential expression of urinary exosomal miRNAs may pose a risk of MsPGN and help distinguish MsPGN patients from controls. Certain miRNA expressions may be associated with disease progression, contributing to the epigenetic understanding of the pathophysiology of MsPGN.

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“…circRNA requires specific binding sites for multiple miRNAs to conduct diverse biological roles. Numerous studies reported that circACTR2 could serve as sponge for several miRNAs, such as miR-561 and miR-205-5p [ 21 , 25 ]. Our study firstly demonstrated that miR-221-3p was a direct target of circACTR2.…”

Section: Discussionmentioning

confidence: 99%

circACTR2 attenuates gemcitabine chemoresiatance in pancreatic cancer through PTEN mediated PI3K/AKT signaling pathway

et al. 2023

Biol Direct

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Background Recently, accumulating studies have unveiled that circRNAs exert critical function in a variety of tumor biological processes including chemoresistance. Our previous study has found circACTR2 is significantly down-regulated in acquired gemcitabine (GEM)- resistant pancreatic cancer (PC) cells, which has not been well-explored. Our study aimed to research the function and molecular mechanism of circACTR2 in PC chemoresistance. Methods qRT-PCR and western blot analysis was performed to detect gene expression. The effect of circACTR2 on PC GEM resistance were examined by CCK-8 and flow cytometry assays. Whether circACTR2 could sponge miR-221-3p and regulate PTEN expression were determined by bioinformatics analysis, RNA pull-down, and Dual-luciferase reporter assay. Results circACTR2 was notably down-regulated in a panel of GEM-resistant PC cells lines, and negatively associated with aggressive phenotype and poor prognosis of PC. circACTR2 downregulation contributed to GEM chemoresistance of PC cells with decreased S phase ratio of cell cycle and cell apoptosis, as confirmed by gain- and loss-of-function assays in vitro. In addition, circACTR2 overexpression retarded GEM resistance in vivo. Further, circACTR2 acted as a ceRNA against miR-221-3p, which directly targeted PTEN. The mechanistic studies revealed that loss of circACTR2 promoted GEM resistance in PC through activating the PI3K/AKT signaling pathway by downregulating PTEN expression in a miR-221-3p dependent manner. Conclusions circACTR2 reversed the chemoresistance of PC cells to GEM through inhibiting PI3K/AKT signaling pathway by sponging miR-221-3p and upregulating PTEN expression.

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Circ-ACTR2 aggravates the high glucose-induced cell dysfunction of human renal mesangial cells through mediating the miR-205-5p/HMGA2 axis in diabetic nephropathy

Cited by 23 publications

References 35 publications

Circular RNAs in diabetes and its complications: Current knowledge and future prospects

Circular RNAs in diabetes and its complications: Current knowledge and future prospects

Differential expression profile of urinary exosomal microRNAs in patients with mesangial proliferative glomerulonephritis

circACTR2 attenuates gemcitabine chemoresiatance in pancreatic cancer through PTEN mediated PI3K/AKT signaling pathway

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