Hsa_circ_0088233 Alleviates Proliferation, Migration, and Invasion of Prostate Cancer by Targeting hsa-miR-185-3p

Abstract

Prostate cancer is the most common malignant tumor of the urinary system. The mechanisms of the initiation and progression of prostate cancer have not been fully elucidated. Increasing evidence suggests that circular RNAs (circRNAs) are involved in cancer pathogenesis. In this study, we aimed to identify differentially expressed circRNAs in prostate cancer tissues and explored the role of circRNAs in the pathogenesis of prostate cancer. By screening a circRNA microarray assay, we found that circ_0088233 was upregulated in prostate cancer tissues compared to adjacent normal tissues, and this upregulation can be verified in 46 pairs of prostate cancer and adjacent normal tissues examined using quantitative reverse transcription-PCR. The level of circ_0088233 correlated with the TNM stage. Knockdown of circ_0088233 reduced cell proliferation, migration, invasion, and induced G1 phase arrest and apoptosis. In addition, miR-185-3p was identified as the downstream target of circ_0088233 using luciferase reporter assays and a biotinylated circ_0088233 probe pull-down assay. The miR-185-3p level showed a negative correlation with the circ_0088233 level in prostate cancer tissues. Overexpression of circ_0088233 blocked the effects of miR-185-3p on cell proliferation, migration, invasion, cell cycle, and apoptosis. In conclusion, circ_0088233 may function as an oncogene and play an oncogenic role by sponging hsa-miR-185-3p. This study increases the understanding of circRNAs in the progression of prostate cancer. These results implicate circ_0088233 as a potential therapeutic target for prostate cancer.

Circular RNA CircPPP1CB Suppresses Tumorigenesis by Interacting With the MiR-1307-3p/SMG1 Axis in Human Bladder Cancer

“…Circular RNAs were first observed in eukaryotic cells approximately 40 years ago and were regarded as by-products or junk of abnormal splicing ( Haddad and Lorenzen, 2019 ). However, as the studies of circRNAs move along, numerous circRNAs were verified to be dysregulated and modulated biological functions in human cancers, including colorectal cancer ( Xu H. et al, 2020 ), cervical adenocarcinoma ( Xu J. et al, 2020 ), hepatocellular carcinoma ( Huang et al, 2020 ), prostate cancer ( Deng Z.H. et al, 2020 ), and BC ( Wang et al, 2020a ).…”

The Emerging Role of Circular RNAs in Prostate Cancer: A Systematic Review

“…Liu et al suggested that circHIPK3 expression was upregulated in PCa and promoted G2/M transition by sponging miR-338-3p (Liu et al, 2020 ). Deng et al claimed that the suppression of circ_0088233 reduced cell proliferation and induced G1 phase arrest and apoptosis through targeting hsa-miR-185-3p (Deng et al, 2020 ). Zhang et al indicated that the knockdown of circ_0057553 could inhibit cell viability and facilitate apoptosis (Zhang et al, 2020e ).…”

CircGNG4 Promotes the Progression of Prostate Cancer by Sponging miR-223 to Enhance EYA3/c-myc Expression

“…In previous studies, a number of circRNAs have been found to play critical roles in prostate cancer progression. For instance, circRNA nucleolar and coiled-body phosphoprotein 1, circRNA formin 1, circ-0016068, circ_0088233 and circ0005276 are all upregulated in prostate cancer and promote the development of prostate cancer ( Feng et al, 2019 ; Chen et al, 2020 ; Deng et al, 2020 ; Li et al, 2020 ; Shan et al, 2020 ). The findings of the present study extend the current understanding of the function of circRNAs in prostate cancer.…”