CircGNG4 Promotes the Progression of Prostate Cancer by Sponging miR-223 to Enhance EYA3/c-myc Expression

Abstract

Patients diagnosed with prostate cancer often have a poor prognosis and limited treatment options, as the specific pathogenesis remains to be elucidated. Circular RNA (circRNA) is a type of non-coding RNA that interacts with microRNA (miRNA/miR) and transcription factors to regulate gene expression. However, little is known about specific circRNAs that serve roles in the pathogenesis of prostate cancer. Findings of the present study confirmed that circRNA G protein subunit γ 4 (circGNG4) was upregulated in prostate cancer tissues and cell lines. Knockdown of circGNG4 inhibited the malignant behavior of prostate cancer cells. Furthermore, bioinformatics were used to predict targeting interactions between circGNG4 or miR-223 and EYA transcriptional coactivator and phosphatase 3 (EYA3)/c-Myc mRNA. miR-223 inhibited the malignant behavior of prostate cancer cells, while EYA3/c-Myc had the opposite effect. circGNG4 enhanced the expression of EYA3/c-Myc by sponging miR-223 to promote the growth of prostate cancer tumors in vivo. In conclusion, the circGNG4/miR-223/EYA3/c-Myc regulatory pathway promoted the malignant progression of prostate cancer. The results of the present study may provide potential new targets for the diagnosis or treatment of prostate cancer.

miR-342-5p downstream to Notch enhances arterialization of endothelial cells in response to shear stress by repressing MYC

“… 32 , 33 Bioinformatic analysis has shown that MYC is not a direct target of miR-342. However, the 3′-UTR of EYA3, which increases MYC protein stability by dephosphorylating threonine 58 (Thr58) and phosphorylating serine 62 (Ser62) of MYC via protein phosphatase 2A, 34 , 35 harbors an miR-342 recognition site ( Figure S7 A). Next, we assessed whether miR-342 influences MYC stability.…”

Circ_0006324 regulates cell proliferation, cell‐cycle progression, apoptosis, and glycolysis of non‐small cell lung cancer cells through miR‐496/TRIM59 axis

“…[ 14 ] circGNG4 worked as a potent driver in prostate cancer by EYA3/c‐Myc pathway via miR‐223 competition. [ 15 ] Circ_001422 sponged miR‐195‐5p to expedite the tumorigenesis and progression of osteosarcoma by fibroblast growth factor 2/phosphatidylinositol 3‐kinase pathway. [ 16 ] In NSCLC, circ_0003222 and circ_0060937 have been claimed to be aberrantly expressed and are strongly linked to the occurrence of NSCLC.…”

Circ_0076305 facilitates prostate cancer development via sponging miR‐411‐5p and regulating PGK1

“…CircRNAs are abnormally expressed in many malignant tumours and have critical roles in regulation of various pathological and physiological processes in cancers (Arnaiz et al, 2019; Vo et al, 2019). Additionally, some circRNAs have been found to be implicated in PCa tumorigenesis via acting as tumour promoters or suppressors (Wang et al, 2021; Xu et al, 2021). Circ_0076305 is derived from progastricsin (PGC) gene and located on chr6:41704448–41712252.…”