Hsa-mir-548 family expression in human reproductive tissues

Rooda, Ilmatar; Kaselt, Birgitta; Liivrand, Maria; Smolander, Olli‐Pekka; Salumets, Andres; Velthut-Meikas, Agne

doi:10.1186/s12863-021-00997-w

“…According to the findings of Peng et al, hsa-miR-8085 is a predicted miRNA that regulates HOXC10 expression, which plays a significant role in ovarian cancer metastasis ( 38 ). The highest expression levels of hsa-miR-548w were discovered in ovarian cumulus granulosa cell (CGC) and mural granulosa cell (MGC) samples according to small RNA high-throughput sequencing performed by Rooda et al ( 39 ). In line with the results of Rooda et al ( 39 ), upregulation of hsa-miR-548w was observed in our study in PCOS patients.…”

Section: Discussionmentioning

confidence: 99%

Unveiling Key Biomarkers and Therapeutic Drugs in Polycystic Ovary Syndrome (PCOS) Through Pathway Enrichment Analysis and Hub Gene-miRNA Networks

Heidarzadehpilehrood,

Pirhoushiaran,

Binti Osman

et al. 2023

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Background: Polycystic ovary syndrome (PCOS) affects women of reproductive age globally with an incidence rate of 5% - 26%. Growing evidence reports important roles for microRNAs (miRNAs) in the pathophysiology of granulosa cells (GCs) in PCOS. Objectives: The objectives of this study were to identify the top differentially expressed miRNAs (DE-miRNAs) and their corresponding targets in hub gene-miRNA networks, as well as identify novel DE-miRNAs by analyzing three distinct microarray datasets. Additionally, functional enrichment analysis was performed using bioinformatics approaches. Finally, interactions between the 5 top-ranked hub genes and drugs were investigated. Methods: Using bioinformatics approaches, three GC profiles from the gene expression omnibus (GEO), namely gene expression omnibus series (GSE)-34526, GSE114419, and GSE137684, were analyzed. Targets of the top DE-miRNAs were predicted using the multiMiR R package, and only miRNAs with validated results were retrieved. Genes that were common between the ''DE-miRNA prediction results'' and the ''existing tissue DE-mRNAs'' were designated as differentially expressed genes (DEGs). Gene ontology (GO) and pathway enrichment analyses were implemented for DEGs. In order to identify hub genes and hub DE-miRNAs, the protein-protein interaction (PPI) network and miRNA-mRNA interaction network were constructed using Cytoscape software. The drug-gene interaction database (DGIdb) database was utilized to identify interactions between the top-ranked hub genes and drugs. Results: Out of the top 20 DE-miRNAs that were retrieved from the GSE114419 and GSE34526 microarray datasets, only 13 of them had "validated results" through the multiMiR prediction method. Among the 13 DE-miRNAs investigated, only 5, namely hsa-miR-8085, hsa-miR-548w, hsa-miR-612, hsa-miR-1470, and hsa-miR-644a, demonstrated interactions with the 10 hub genes in the hub gene-miRNA networks in our study. Except for hsa-miR-612, the other 4 DE-miRNAs, including hsa-miR-8085, hsa-miR-548w, hsa-miR-1470, and hsa-miR-644a, are novel and had not been reported in PCOS pathogenesis before. Also, GO and pathway enrichment analyses identified ''pathogenic E. coli infection'' in the Kyoto encyclopedia of genes and genomes (KEGG) and ''regulation of Rac1 activity'' in FunRich as the top pathways. The drug-hub gene interaction network identified ACTB, JUN, PTEN, KRAS, and MAPK1 as potential targets to treat PCOS with therapeutic drugs. Conclusions: The findings from this study might assist researchers in uncovering new biomarkers and potential therapeutic drug targets in PCOS treatment.

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“…Another interesting micro‐RNA superfamily found altered in the lesion skin of HS patients was the hsa‐miR‐548 family, a large and poorly conserved primate‐specific miRNA family consisting of 76 members 95 . miR‐548a‐3p has been greatly investigated in the past 5 years for its potential to become a screening biomarker for cancers.…”

Section: Discussionmentioning

confidence: 99%

“…94 The microRNA-548 family Another interesting micro-RNA superfamily found altered in the lesion skin of HS patients was the hsa-miR-548 family, a large and poorly conserved primate-specific miRNA family consisting of 76 members. 95 miR-548a-3p has been greatly investigated in the past 5 years for its potential to become a screening biomarker for cancers. It has displayed a relevant possibility to segregate patients with or without prostate cancer, 96 mesothelioma 97 and gastric cancer.…”

Section: Wnt/b-catenin Signallingmentioning

confidence: 99%

Methylated miRNAsmay serve as potential biomarkers and therapeutic targets for hidradenitis suppurativa

Radhakrishna

¹

,

Uppala

²

,

Jhala

³

et al. 2022

Acad Dermatol Venereol

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Background Hidradenitis suppurativa (HS) is a chronic inflammatory disease influenced by genetics, non‐genetic and environmental factors that modulate miRNA expression. Currently, no miRNA data are available for HS. In this study, we profiled DNA methylation patterns of miRNA genes associated with HS susceptibility. Objectives Identify miRNA gene methylation profiles associated with HS susceptibility. This study examined the methylation patterns of DNAs from 24 healthy controls and 24 patients with HS using Illumina Infinium MethylationEPIC BeadChip array analysis. Methylation patterns of miRNA genes were analysed using KEGG pathway analysis to explore the inversely correlated pathways regulated by miRNAs. Results We identified 60 CpG sites representing 65 unique microRNA genes including 54 hypomethylated and 6 hypermethylated CpGs as potentially associated with HS. Some of these CpGs were found to be critical for skin function, such as miR‐29, miR‐200, miR‐205, miR‐548 and miR‐132. The miR‐192 is implicated in non‐alcoholic fatty liver disease. The miR‐200c gene was identified as a vital determinant in regulating skin repair after injury and may contribute to age‐associated alterations in wound repair. miR‐132 was significantly upregulated during the inflammation phase of wound repair, enhancing the activity of STAT3 and ERK pathways that promote keratinocyte proliferation. Conclusions Epigenetically altered microRNA genes are implicated in wound healing, inflammation, keratinocyte proliferation and wound modulation. This is the first study to analyse methylation profiles of miRNA genes in the HS population, highlighting the unique role that miRNAs might play in diagnosing and treating HS.

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“…The substitution T > A of rs221634 causes losing a binding site of hsa-mir-548 family in the 3′ UTR of human LIN28B gene, as analyzed by SNPinfo ( https://manticore.niehs.nih.gov/ ). The hsa-mir-548 family is highly expressed in human reproductive tissues [ 34 ] and is a pivotal suppressor of cell growth [ 35 – 37 ]. So we can also explain our results by that rs221634 T > A polymorphism is the real genetic cause of polyp recurrence.…”

Section: Discussionmentioning

confidence: 99%

LIN28B Polymorphisms Confer a Higher Postoperative Recurrence Risk in Reproductive-Age Women with Endometrial Polyps

Lu

¹

,

Li

²

,

Niu

³

et al. 2022

Disease Markers

1

0

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The RNA-binding protein LIN28B is an important factor for cell proliferation. Because LIN28B polymorphisms have been shown to be relative with the recurrence of some hyperplastic diseases, we hypothesized that genetic variants of LIN28B gene were associated with postoperative recurrence risk in reproductive-age women with endometrial polyps (EP). In a hospital-based cohort of 351 reproductive female patients underwent hysteroscopic polypectomies between May 2018 and Jan 2020, we genotyped two common polymorphisms in LIN28B gene ( rs 369065 C > T and rs 314280 A > G ) and analyzed their associations with the risk of postoperative recurrence in multiple Cox regression model. When followed up to Jun 2021, carries of rs369065 TT genotype had an increased risk of polyp recurrence (adjusting hazard ratio HR = 1.883 , 95% confidence interval CI = 1.033 − 3.434 ) and had a shorter time to recurrence (median time 352 vs. 342 days, log-rank P < 0.01 ), compared to the CC/CT genotype. Further stratification analysis showed that the increased risk of rs369065 TT genotype was more evident in patients who were older than 33 years (adjusted HR = 2.597 , 95 % CI = 1.037 − 6.505 ), had a single polyp (adjusted HR = 2.545 , 95 % CI = 1.059 − 6.113 ), and had smaller polyps (<1.2 cm, adjusted HR = 2.708 , 95 % CI = 1.042 − 7.043 ). However, no significant association between rs 314280 A > G polymorphism and the risk of polyp recurrence was found. Our study suggests that rs369065 TT genotype of LIN28B gene is associated with an increased postoperative recurrence risk in EP patients, especially in those with fewer and smaller polyps. These findings implicate a precise choice of clinical counseling and decision making. Larger studies in different ethnic populations are warranted.

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Hsa-mir-548 family expression in human reproductive tissues

Cited by 7 publications

References 66 publications

Unveiling Key Biomarkers and Therapeutic Drugs in Polycystic Ovary Syndrome (PCOS) Through Pathway Enrichment Analysis and Hub Gene-miRNA Networks

Unveiling Key Biomarkers and Therapeutic Drugs in Polycystic Ovary Syndrome (PCOS) Through Pathway Enrichment Analysis and Hub Gene-miRNA Networks

Methylated miRNAsmay serve as potential biomarkers and therapeutic targets for hidradenitis suppurativa

LIN28B Polymorphisms Confer a Higher Postoperative Recurrence Risk in Reproductive-Age Women with Endometrial Polyps

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