hsa‐mir‐133a‐2 promotes the proliferation and invasion of cervical cancer cells by targeting the <scp>LAMB3</scp>‐mediated <scp>PI3K</scp>/<scp>ATK</scp> pathway

Sui, Xiaoyu; Sun, Yurong; Zhang, Guiyu; Chi, Na; Guan, Yitong; Wang, Dan; Li, Xiulan

doi:10.1002/cam4.5380

Cited by 8 publications

(5 citation statements)

References 44 publications

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“…The GEP data presented in this study reveal, for the first time, the expression of MMP7, LAMB3 , and LAMC2 RNA in PTL. The epigenetic regulation of LAMB3 and LAMC2 , as previously reported in solid tumors, did not align with the findings of the current research 68 . Therefore, the findings presented necessitate additional large‐scale investigations.…”

Section: Discussioncontrasting

confidence: 88%

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Primary testicular lymphoma demonstrates overexpression of the Wilms tumor 1 gene and different mRNA and miRNA expression profiles compared to nodal diffuse large B‐cell lymphoma

Mansoor

Akhter

Shabani‐Rad

et al. 2023

Hematological Oncology

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Diffuse large B‐cell lymphoma (DLBCL) shows a high degree of clinical and biological heterogeneity. Primary testicular lymphoma (PTL) is an extranodal variant of DLBCL associated with a higher risk of recurrence, including contralateral testicles and central nervous system sanctuary sites. Several molecular aberrations, including somatic mutation of MYD88, CD79B, and upregulation of NF‐kB, PDL‐1, and PDL‐2, are thought to contribute to the pathogenesis and poor prognosis of PTL. However, additional biomarkers are needed that may improve the prognosis and help understand the PTL biology and lead to new therapeutic targets. RNA from diagnostic tissue biopsies of the PTL‐ABC subtype and matched nodal DLBCL‐ABC subtype patients was evaluated by mRNA and miRNA expression. Screening of 730 essential oncogenic genes was performed, and their epigenetic connections were examined using the nCounter PAN‐cancer pathway, and Human miRNA assays with the nCounter System (NanoString Technologies). PTL and nodal DLBCL patients were comparable in age, gender, and putative cell of origin (p > 0.05). Wilms tumor 1 (WT1) expression in PTL exceeded that in nodal DLBCL (>6‐fold; p = 0.01, FDR <0.01) and WT1 associated pathway genes THBS4, PTPN5, PLA2G2A, and IFNA17 were upregulated in PTL (>2.0‐fold, p < 0.01, FDR <0.01). Additionally, miRNAs targeting WT1 (hsa15a‐5p, hsa‐miR‐16‐5p, has‐miR‐361‐5p, has‐miR‐27b‐3p, has‐miR‐199a‐5p, has‐miR‐199b‐5p, has‐miR‐132‐3p, and hsa‐miR‐128‐3p) showed higher expression in PTL compared to nodal DLBCL (≥2.0‐fold; FDR 0.01). Lower expression of BMP7, LAMB3, GAS1, MMP7, and LAMC2 (>2.0‐fold, p < 0.01) was observed in PTL compared to nodal DLBCL. This research revealed higher WT1 expression in PTL relative to nodal DLBCL, suggesting that a specific miRNA subset may target WT1 expression and impact the PI3k/Akt pathway in PTL. Further investigation is needed to explore WT1's biological role in PTL and its potential as a therapeutic target.

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Section: Discussioncontrasting

confidence: 88%

“…The epigenetic regulation of LAMB3 and LAMC2, as previously reported in solid tumors, did not align with the findings of the current research. 68 Therefore, the findings presented necessitate additional large-scale investigations.…”

mentioning

confidence: 99%

Primary testicular lymphoma demonstrates overexpression of the Wilms tumor 1 gene and different mRNA and miRNA expression profiles compared to nodal diffuse large B‐cell lymphoma

Mansoor

Akhter

Shabani‐Rad

et al. 2023

Hematological Oncology

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“…We are not aware of studies at this time analysing the direct association between the purinergic receptor P2RY2 and PAAD; this receptor is known to upregulate selinexor, which, in turn, activates PI3K/AKT signalling in acute myeloid leukaemia ( Lin et al, 2022 ). It is well-known that abnormal activation of the PI3K-Akt signalling pathway is related to malignancy, there is also a relationship between PI3K and receptor tyrosine kinases upstream of PI3K ( Noorolyai et al, 2019 ), and furthermore, the KEGG pathway analysis of LM332-co-expressed gene revealed that a correlation with PI3K-Akt signalling pathway genes and numerous in vitro studies have linked LM332 with PI3K activation ( Yazlovitskaya et al, 2015 ; Zhang et al, 2019 ; Sui et al, 2022 ). Therefore, we suggest that the overexpression of LM332 in PAAD tumours represents a more widespread upregulation of genes promoting tumorigenesis poor outcomes.…”

Section: Discussionmentioning

confidence: 99%

Laminin 332 expression levels predict clinical outcomes and chemotherapy response in patients with pancreatic adenocarcinoma

Sari,

Gulbey,

Hamill

2023

Front. Cell Dev. Biol.

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Poor outcomes and chemotherapy resistance for patients with pancreatic adenocarcinoma (PAAD) are a challenge worldwide, and new or improved prognostic biomarkers are urgently required. Individual laminin family members have been established as cancer-associated markers, predicting patient outcomes in many cancer types, including PAAD. Here, we used multiple modalities including RNAseq and gene chip, and genomic and proteomic data to examine the relationships of all laminin genes in PAAD with clinical outcomes. These analyses identified that LAMA3, LAMB3, and LAMC2 expression levels are increased at the mRNA and protein levels in PAAD tumours with evidence of co-regulation. Increased expression of all three genes was associated with decreased promoter methylation status, TP53 mutations, and altered receptor tyrosine kinase (RTK) pathways. Clinically, high LAMA3, LAMB3, and LAMC2 transcript abundance was each related to an advanced histological grade. Moreover, high expression of these genes individually predicted poor patient survival, while a signature of combined high expression of LAMA3, LAMB3, and LAMC2 was a stronger predictor of patient outcomes than each gene alone. Interestingly, cell lines with high expression of LM332 chains were not sensitive to the commonly used PAAD chemotherapy drugs paclitaxel and gemcitabine; however, increased sensitivity was evident for erlotinib, afatinib, gefitinib, and cetuximab epidermal growth factor (EGFR) RTK inhibitors. To explore possible mechanisms, we investigated co-expressed genes, identifying eight hub genes, namely, GJB3, ITGB6, SERPINB5, GPRC5A, PLEK2, TMPRSS4, P2RY2, and TRIM29, which are co-expressed with all three of LAMA3, LAMB3, and LAMC2. Of these, only SERPINB5 provided a stronger predictive value than the laminin-encoding genes. Together, these multiple integrated analyses suggest that the combined expression of LM332 is a useful prognostic biomarker for PAAD and could help patient stratification and therapeutic selection.

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“…Specifically, a particular set of miRNAs (defined as epi-miRNAs) can indirectly affect the expression of tumor suppressor genes by influencing epigenetic mechanism effectors such as DNA methyltransferases, histone deacetylases, and polyoma suppressor complex genes, which are also controlled by epigenetic factors ( Fabbri and Calin, 2010 ). Further information on the mechanism underlying the occurrence of CC based on miRNAs has recently been obtained ( Sui et al, 2022 ). According to current findings, the mechanisms of miRNAs in CC development include the following aspects.…”

Section: Mirna Interference With CC Developmentmentioning

confidence: 99%

The mechanisms and diagnostic potential of lncRNAs, miRNAs, and their related signaling pathways in cervical cancer

Sun

Song

et al. 2023

Front. Cell Dev. Biol.

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Cervical cancer (CC), the fourth most prevalent type of cancer among women worldwide, is associated with high rates of morbidity and mortality. Due to the long period of latency in CC, most patients are already in the middle to late stages when initially diagnosed, which greatly reduces the clinical cure rate and quality of survival, thus resulting in poor outcomes. In recent years, with continuous exploration in the fields of bioinformatics and molecules, it has been found that ncRNAs, including miRNAs and lncRNAs, without the ability to translate proteins are capable of activating or inhibiting certain signaling pathways by targeting and modulating the level of expression of proteins involved in these signaling pathways. ncRNAs play important roles in assisting with diagnosis, drug administration, and prediction of prognosis during CC progression. As an entry point, the mechanisms of interaction between miRNAs, lncRNAs, and signaling pathways have long been a focus in basic research relating to CC, and numerous experimental studies have confirmed the close relationship of miRNAs, lncRNAs, and signaling pathways with CC development. Against this background, we summarize the latest advances in the involvement of lncRNA- and miRNA-related signaling pathways in the development of CC to provide guidance for CC treatment.

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hsa‐mir‐133a‐2 promotes the proliferation and invasion of cervical cancer cells by targeting the LAMB3‐mediated PI3K/ATK pathway

Cited by 8 publications

References 44 publications

Primary testicular lymphoma demonstrates overexpression of the Wilms tumor 1 gene and different mRNA and miRNA expression profiles compared to nodal diffuse large B‐cell lymphoma

Primary testicular lymphoma demonstrates overexpression of the Wilms tumor 1 gene and different mRNA and miRNA expression profiles compared to nodal diffuse large B‐cell lymphoma

Laminin 332 expression levels predict clinical outcomes and chemotherapy response in patients with pancreatic adenocarcinoma

The mechanisms and diagnostic potential of lncRNAs, miRNAs, and their related signaling pathways in cervical cancer

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