HRS1/HHOs GARP transcription factors and reactive oxygen species are regulators of Arabidopsis nitrogen starvation response

Safi, Alaeddine; Medici, Anna; Szponarski, Wojciech; Marshall‐Colón, Amy; Ruffel, Sandrine; Gaymard, Frédéric; Coruzzi, Gloria M.; Lacombe, Benoı̂t; Krouk, Gabriel

doi:10.1101/164277

Cited by 12 publications

(11 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This expression pattern was similar to that of OsNIGT1 [38]. Transient transcription dual-luciferase assay showed that ZmNIGT1 and SbNIGT1 had transcriptional inhibitory activity, which was consistent with Arabidopsis NIGT1 subfamily transcription factors [54,59]. These results suggested that ZmNIGT1 and its homologies in other plants are negative regulators under conditions of high N availability.…”

Section: Zmnigt1 and Sbnigt1 Are Transcriptional Repressors Of Nitratsupporting

confidence: 71%

“…Several regulated genes were associated with phosphate transport and starvation responses. Arabidopsis NIGT1s shares many features with ZmNIGT1, such as nitrate-inducible expression, transcriptional repressor activity, binding motifs, and auto-negative feedback regulation [54,58,59]. Three genes encode sulfate and potassium transporters, implying that ZmNIGT1 may be involved in the metabolism of various nutrients.…”

Section: Zmnigt1 and Sbnigt1 Are Transcriptional Repressors Of Nitratmentioning

confidence: 99%

See 1 more Smart Citation

Cross-Species Root Transcriptional Network Analysis Highlights Conserved Modules in Response to Nitrate between Maize and Sorghum

Du¹,

Ning²,

He³

et al. 2020

IJMS

View full text Add to dashboard Cite

Plants have evolved complex mechanisms to respond to the fluctuation of available nitrogen (N) in soil, but the genetic mechanisms underlying the N response in crops are not well-documented. In this study, we generated a time series of NO3−-mediated transcriptional profiles in roots of maize and sorghum, respectively. Using weighted gene co-expression network analysis, we identified modules of co-expressed genes that related to NO3− treatments. A cross-species comparison revealed 22 conserved modules, of which four were related to hormone signaling, suggesting that hormones participate in the early nitrate response. Three other modules are composed of genes that are mainly upregulated by NO3− and involved in nitrogen and carbohydrate metabolism, including NRT, NIR, NIA, FNR, and G6PD2. Two G2-like transcription factors (ZmNIGT1 and SbNIGT1), induced by NO3− stimulation, were identified as hub transcription factors (TFs) in the modules. Transient assays demonstrated that ZmNIGT1 and SbNIGT1 are transcriptional repressors. We identified the target genes of ZmNIGT1 by DNA affinity-purification sequencing (DAP-Seq) and found that they were significantly enriched in catalytic activity, including carbon, nitrogen, and other nutrient metabolism. A set of ZmNIGT1 targets encode transcription factors (ERF, ARF, and AGL) that are involved in hormone signaling and root development. We propose that ZmNIGT1 and SbNIGT1 are negative regulators of nitrate responses that play an important role in optimizing nutrition metabolism and root morphogenesis. Together with conserved N responsive modules, our study indicated that, to encounter N variation in soil, maize and sorghum have evolved an NO3−-regulatory network containing a set of conserved modules and transcription factors.

show abstract

Section: Zmnigt1 and Sbnigt1 Are Transcriptional Repressors Of Nitratsupporting

confidence: 71%

Section: Zmnigt1 and Sbnigt1 Are Transcriptional Repressors Of Nitratmentioning

confidence: 99%

Cross-Species Root Transcriptional Network Analysis Highlights Conserved Modules in Response to Nitrate between Maize and Sorghum

Du¹,

Ning²,

He³

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Furthermore, ChIP-qPCR experiments confirmed the association of NIGT1 with the NRT2.5, GLN1;4, and GDH3 promoters at the NIGT1 binding motifs ( Figures 8C and 8D). Using different methods, Safi et al (2018) recently reported that NRT2.4, NRT2.5, and GDH3 are directly repressed by NIGT1s. These results strongly support the validity of our lists as enriched for NIGT1 direct targets.…”

Section: Identification Of Nigt1 Target Genesmentioning

confidence: 99%

Repression of Nitrogen Starvation Responses by Members of the Arabidopsis GARP-Type Transcription Factor NIGT1/HRS1 Subfamily

et al. 2018

View full text Add to dashboard Cite

Nitrogen (N) is often a limiting nutrient whose availability determines plant growth and productivity. Because its availability is often low and/or not uniform over time and space in nature, plants respond to variations in N availability by altering uptake and recycling mechanisms, but the molecular mechanisms underlying how these responses are regulated are poorly understood. Here, we show that a group of GARP G2-like transcription factors, NITRATE-INDUCIBLE, GARP-TYPE TRANSCRIPTIONAL REPRESSOR1/HYPERSENSITIVE TO LOW Pi-ELICITED PRIMARY ROOT SHORTENING1 proteins (NIGT1/HRS1s), are factors that bind to the promoter of the N starvation marker and repress an array of N starvation-responsive genes under conditions of high N availability. Transient assays and expression analysis demonstrated that NIGT1/HRS1s are transcriptional repressors whose expression is regulated by N availability. We identified target genes of the NIGT1/HRS1s by genome-wide transcriptome analyses and found that they are significantly enriched in N starvation response-related genes, including N acquisition, recycling, remobilization, and signaling genes. Loss of resulted in deregulation of N acquisition and accumulation. We propose that NIGT1/HRS1s are major regulators of N starvation responses that play an important role in optimizing N acquisition and utilization under fluctuating N conditions.

show abstract

“…and HRS1/HHOs control N use (Baena-González et al, 2007;Rubin et al, 2009;Araus et al, 2016;Safi et al, 2018). The transcription factor TCP20, which is not involved in the PNR per se, physically interacts with NLP6/7 and regulates the expression of NO 3 − -responsive genes and a cell cycle marker gene (Li et al, 2005;Guan et al, 2014Guan et al, , 2017.…”

mentioning

confidence: 99%

Responses to Systemic Nitrogen Signaling in Arabidopsis Roots Involve trans-Zeatin in Shoots

et al. 2018

Self Cite

View full text Add to dashboard Cite

Plants face temporal and spatial variation in nitrogen (N) availability. This includes heterogeneity in soil nitrate (NO) content. To overcome these constraints, plants modify their gene expression and physiological processes to optimize N acquisition. This plasticity relies on a complex long-distance root-shoot-root signaling network that remains poorly understood. We previously showed that cytokinin (CK) biosynthesis is required to trigger systemic N signaling. Here, we performed split-root experiments and used a combination of CK-related mutant analyses, hormone profiling, transcriptomic analysis, NO uptake assays, and root growth measurements to gain insight into systemic N signaling in By comparing wild-type plants and mutants affected in CK biosynthesis and ABCG14-dependent root-to-shoot translocation of CK, we revealed an important role for active-zeatin (Z) in systemic N signaling. Both rapid sentinel gene regulation and long-term functional acclimation to heterogeneous NO supply, including NO transport and root growth regulation, are likely mediated by the integration of content in shoots. Furthermore, shoot transcriptome profiling revealed that glutamate/glutamine metabolism is likely a target ofZ root-to-shoot translocation, prompting an interesting hypothesis regarding shoot-to-root communication. Finally, this study highlights Z-independent pathways regulating gene expression in shoots as well as NO uptake activity in response to total N deprivation.

show abstract

HRS1/HHOs GARP transcription factors and reactive oxygen species are regulators of Arabidopsis nitrogen starvation response

Abstract: Abstract

Cited by 12 publications

References 74 publications

Cross-Species Root Transcriptional Network Analysis Highlights Conserved Modules in Response to Nitrate between Maize and Sorghum

Cross-Species Root Transcriptional Network Analysis Highlights Conserved Modules in Response to Nitrate between Maize and Sorghum

Repression of Nitrogen Starvation Responses by Members of the Arabidopsis GARP-Type Transcription Factor NIGT1/HRS1 Subfamily

Responses to Systemic Nitrogen Signaling in Arabidopsis Roots Involve trans-Zeatin in Shoots

Contact Info

Product

Resources

About