Repression of Nitrogen Starvation Responses by Members of the Arabidopsis GARP-Type Transcription Factor NIGT1/HRS1 Subfamily

Kiba, Takatoshi; Inaba, Jun; Kudo, Toru; Ueda, Norihiro; Konishi, Mineko; Mitsuda, Nobutaka; Takiguchi, Yuko; Kondou, Youichi; Yoshizumi, Takeshi; Ohme‐Takagi, Masaru; Matsui, Minami; Yano, Kentarô; Yanagisawa, Shuichi; Sakakibara, Hitoshi

doi:10.1105/tpc.17.00810

Cited by 158 publications

(145 citation statements)

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“…We considered that the grafted scions were subject to inorganic nutrient starvation stress before forming the vasculature connection between the scion and stock, and examined the expression level of marker genes that respond to mineral nutrient starvation stress. We focused on the ERD5, SGT1 , and NRT2.5 genes of At among the nutrient deficiency responsive genes, reported previously (Hammond et al ., 2003; Kiba et al ., 2018), and identified their orthologs in Nb by tblastx search. The orthologs of Nb of AT3G30775 ( ERD5 ), AT2G43820 ( SGT1 ) and AT1G12940 ( NRT2.5 ) were Niben101Scf00390g02001, Niben101Scf05415g00003 and Niben101Scf12671g00007 , respectively.…”

Section: Resultsmentioning

confidence: 99%

Autophagy is induced during plant grafting for wound healing

Kurotani

Tabata

Kawakatsu

et al. 2020

Preprint

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l Grafting is an important technique in agriculture to obtain several good traits such as high disease tolerance and high yield by exchanging root system. However, the underlined cellular processes to compensate the wound damage and repair tissues were largely unknown. l We analyzed two graft combinations: Nicotiana benthamiana (Nb) homograft as a compatible, wound repairing model and Nb heterograft with Arabidopsis thaliana (At)as an incompatible and more stressful model, which we recently identified as an exceptional maintainable interfamily grafting. l In both graft combinations, nutrient loss was observed in gene expression after grafting, where the level of nutrient loss was more sever in heterografts. Transmission electron micrographs of Nb/At heterografts suggested that microautophagy was induced in cells near the graft boundary. In At seedling micrografting, the fluorescence of autophagy protein marker GFP-ATG8 was highly observed at graft junction especially in cambial region. In At atg2 mutant homografts, growth after grafting decreased compared with wild-type homografts. Moreover, when NbATG5 knockeddown Nb scion was grafted to At stock, the successful rate of grafting was significantly decreased. l Altogether, these results suggest that component of autophagy is induced during grafting and has a role in wound healing.

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Section: Resultsmentioning

confidence: 99%

Autophagy is induced during plant grafting for wound healing

Kurotani

Tabata

Kawakatsu

et al. 2020

Preprint

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“…This idea is supported by the work of (Ma et al, 2015) showing that the regulation of NRT2.4 and NRT2.5 by N starvation depends on CBL7, which is specifically induced by NO 3 - deficiency. Moreover, NIGT/HRS1s have been shown to act as transcriptional repressor of NRT2.4 and NRT2.5 upon NO 3 - treatment (Kiba et al, 2018)Safi et al 2018). Local regulation by NO 3 - would explain why these transporters, unlike NRT2.1 , are always repressed when plants are on 10 mM NO 3 - , regardless of the light conditions (Supplemental Figure 1A).…”

Section: Discussionmentioning

confidence: 99%

“…HY5 has been recently identified as a regulator of NRT2.1 by mediating light promotion of NO 3 - uptake (Chen et al, 2016). HRS1, HHO1, HHO2 and HHO3 are repressors of NRT2.4 and NRT2.5 expression under high N conditions (Kiba et al, 2018)Safi et al 2018)…”

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confidence: 99%

Genome-wide Analysis In Response to N and C Identifies New Regulators for root AtNRT2 Transporters

Ruffel

Chaput

Przybyla-Toscano

et al. 2019

Preprint

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In Arabidopsis thaliana, the High-Affinity Transport System (HATS) for root NO3- uptake depends mainly on four NRT2 transporters, namely NRT2.1, NRT2.2, NRT2.4 and NRT2.5. The HATS is the target of many regulations to coordinate nitrogen (N) acquisition with the N status of the plant and with carbon (C) assimilation through photosynthesis. At the molecular level, C and N signaling pathways have been shown to control gene expression of the NRT2 transporters. Although several regulators of these transporters have been identified in response to either N or C signals, the response of NRT2 genes expression to the interaction of these signals has never been specifically investigated and the underlying molecular mechanisms remain largely unknown. To address this question we used an original systems biology approach to model a regulatory gene network targeting NRT2.1, NRT2.2, NRT2.4 and NRT2.5 in response to N/C signals. Our systems analysis of the data highlighted the potential role of three putative transcription factors, TGA3, MYC1 and bHLH093. Functional analysis of mutants combined with yeast one hybrid experiments confirmed that all 3 transcription factors are regulators of NRT2.4 or NRT2.5 in response to N or C signals.One sentence summaryIdentification of three transcription factors involved in the regulation of NRT2s transporters using a systems biology approach and NRT2.1 as target gene in response to combinations of N/C treatments

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“…Recent studies have found that NITRATE-INDUCIBLE, GARP-TYPE TRANSCRIPTIONAL REPRESSORs (NIGT1.1/1.2/1.3/1.4) downregulate the expression of genes induced by N starvation, including NRT2.1, NRT2.4, NRT2.5, AMT1;1, and AMT1;3. (Medici et al 2015;Kiba et al 2018;Maeda et al 2018). Therefore, we profiled the expression of all candidate genes that are repressed by NIGT1.2 and induced by N starvation, according to the list compiled by Kiba et al (2018) (Fig.…”

Section: Expression Of Genes Encoding Inorganic N Transporters In Rootsmentioning

confidence: 99%

“…Hu et al (2009) reported that the transcript levels of several nitrateresponsive genes are positively correlated with nitrate concentrations in A. thaliana roots. One such gene is the transcriptional repressor NIGT1.4, the translational product of which downregulates the expression of high-affinity N uptake transporters (Medici et al 2015;Kiba et al 2018;Maeda et al 2018). Accordingly, the high-affinity nitrate transporter gene NRT2.1, which is induced by nitrate during the PNR, was shown to be repressed by a nitratedependent feedback signal involving the NRT1.1/NPF6.3/CHL1 transceptor (Bouguyon et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Shoot nitrate underlies a perception of nitrogen satiety to trigger local and systemic signaling cascades in Arabidopsis thaliana

Okamoto

Suzuki

Sugiura

et al. 2018

Soil Science and Plant Nutrition

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Nitrate is an important ion for plant growth and development. It serves not only as a building block for amino acid synthesis but also as a signaling molecule. Changes in the exogenous nitrate concentrations affect the expression of nitrate-responsive genes within minutes. Following these rapid transcriptional events, nitrate and its downstream organic nitrogen (N) compounds accumulate in the plant body, inducing secondary responses to the internal N level. Nevertheless, the respective roles of nitrate and organic N in triggering plant responses to internal N remain to be clarified. Several studies have implied that internal nitrate levels regulate root N uptake independent of the levels of N assimilation products. However, little is known about the specific effects of internal nitrate levels on plant growth and gene expression. To manipulate the internal nitrate levels independently of internal organic N, we grew wildtype Arabidopsis and a nitrate reductase (NR)-null mutant under a series of modified N conditions. Using their shoots and roots, we performed analyses of plant growth and RNA sequencing. The results showed that elevated shoot nitrate accumulation in the NR-null mutant was accompanied by increased expression of nitrate assimilatory genes in the shoots, decreased gene expression of high-affinity nitrate and ammonium uptake transporters in the roots, and decreased lateral root growth. Furthermore, the genes normally induced by N deficiency were significantly downregulated both in the shoots and roots of the NR-null mutant, compared with the wild-type. Our transcriptional profiling suggests that the transcription factors NLP7 and NIGT mediate a wide range of these transcriptional responses. Taken together, we conclude that shoot nitrate acts as a N satiety signal to trigger local and systemic signaling cascades in A. thaliana. The present study illustrates an adaptive strategy of plants to survive in N-limited environments, depending on the residual nitrate storage. ARTICLE HISTORY

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Repression of Nitrogen Starvation Responses by Members of the Arabidopsis GARP-Type Transcription Factor NIGT1/HRS1 Subfamily

Cited by 158 publications

References 78 publications

Autophagy is induced during plant grafting for wound healing

Autophagy is induced during plant grafting for wound healing

Genome-wide Analysis In Response to N and C Identifies New Regulators for root AtNRT2 Transporters

Shoot nitrate underlies a perception of nitrogen satiety to trigger local and systemic signaling cascades in Arabidopsis thaliana

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