HrpZ harpins from different Pseudomonas syringae pathovars differ in molecular interactions and in induction of anion channel responses in Arabidopsis thaliana suspension cells

Haapalainen, Minna; Dauphin, Aurélien; Li, C.-M.; Bailly, Guillaume; Tran, Daniel; Briand, Joël; Bouteau, François; Taira, Suvi

doi:10.1016/j.plaphy.2011.10.022

Cited by 15 publications

(12 citation statements)

References 39 publications

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“…AtGLP genes that respond to pathogens ( Pseudomonas and Phytophthora ) and elicitors [flagellin frgment 22 (Flg22), lipopolysaccharides (LPS), harpin (HrpZ), glutathione S-transferases (GST) and GST-necrosis-inducing phytophthora protein 1 (GST-NPP1)] were analysed using microarray data [48,49,50,51]. The expressions of 8 AtGLP genes were significantly changed under various biotic stress conditions (Figure 7, Table S9).…”

Section: Resultsmentioning

confidence: 99%

Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis

Chen

et al. 2016

IJMS

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Previous studies have shown that germin-like proteins (GLPs) are present ubiquitously in rice and Arabidopsis. However, the understanding regarding their role in development and abiotic/biotic stress resistance remains limited. In the present study, we report genome-wide identification, characterisation, subcellular localization, enzyme activity, and expression analysis of the GLP gene family in rice and Arabidopsis to study their functions. In total, 43 and 32 GLPs in the rice and Arabidopsis genome were identified based on a systematic analysis, respectively. The GLP genes were clustered into six clades based on phylogenetic analysis, and many stress and developmental-related cis-elements were detected in promoters of GLP genes. In addition, subcellular location and superoxide dismutase (SOD) analysis demonstrated that the random selected OsGLP genes on chromosomes 8 and 4 of rice were expressed in the cell wall with SOD activity. Overall, our results showed that tandem duplication events, especially the clusters of tandem duplication genes on chromosome 8 in rice, play a major role in expansion of the GLP family and thus increase our understanding of the role of the GLP family in abiotic/biotic stress and development.

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Section: Resultsmentioning

confidence: 99%

Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis

Chen

et al. 2016

IJMS

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“…HrpZ can bind to phosphatidic acid and, upon insertion into vesicles prepared from plant plasma membranes, it can cause vesicle disruption (Lee et al ., 2001b; Haapalainen et al ., 2011). Despite this obviously different mode of perception for HrpZ, there seems to be some sort of specificity: when HrpZ proteins originating from two different pathovars of P. syringae were administered to A. thaliana , both HrpZ types caused cell death but the modulation of anionic currents was specific, and in a phage display screen for peptide binding, different motif preferences were observed (Haapalainen et al ., 2012). …”

Section: Discussionmentioning

confidence: 99%

“…Whether HrpZ can trigger ETI of the strict sense, is not clear. The finding of pathovar-specific activities in A. thaliana (Haapalainen et al ., 2012) indicates the existence of cognate R-gene products. Due to this conceptual incertainty, the term ‘ETI-like’ response will be used throughout the current work.…”

Section: Introductionmentioning

confidence: 99%

The cytoskeleton is disrupted by the bacterial effector HrpZ, but not by the bacterial PAMP flg22, in tobacco BY-2 cells

Guan

Buchholz

Nick

2013

Journal of Experimental Botany

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Plant innate immunity is composed of two layers. Basal immunity is triggered by pathogen-associated molecular patterns (PAMPs) such as the flagellin-peptide flg22 and is termed PAMP-triggered immunity (PTI). In addition, effector-triggered immunity (ETI) linked with programmed cell death and cytoskeletal reorganization can be induced by pathogen-derived factors, such as the Harpin proteins originating from phytopathogenic bacteria. To get insight into the link between cytoskeleton and PTI or ETI, this study followed the responses of actin filaments and microtubules to flg22 and HrpZ in vivo by spinning-disc confocal microscopy in GFP-tagged marker lines of tobacco BY-2. At a concentration that clearly impairs mitosis, flg22 can induce only subtle cytoskeletal responses. In contrast, HrpZ causes a rapid and massive bundling of actin microfilaments (completed in ~20min, i.e. almost simultaneously with extracellular alkalinization), which is followed by progressive disintegration of actin cables and cytoplasmic microtubules, a loss of cytoplasmic structure, and vacuolar disintegration. Cytoskeletal disruption is proposed as an early event that discriminates HrpZ-triggered ETI-like defence from flg22-triggered PTI.

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“…Arabidopsis thaliana cell suspension cultures (ACSC) are an amenable, simplified model in which to study specific signaling mechanisms that would be too complex in plant tissues or organs. For instance, ACSC were recently used to study sugar signaling (Kunz et al, 2014), MAPK kinase and phosphatase signaling (Schweighofer et al, 2014), chitosan and galacturonide elicitor signaling (Ledoux et al, 2014), photooxidative damage (Gutiérrez et al, 2014), auxin transmembrane transport (Seifertová et al, 2014) and ion channel activity (Haapalainen et al, 2012). ACSC are easily labeled which is convenient for studying metabolic fluxes (Tjellström et al, 2012) like those in lipid phospholipid signaling.…”

Section: Introductionmentioning

confidence: 99%

Salicylic acid modulates levels of phosphoinositide dependent-phospholipase C substrates and products to remodel the Arabidopsis suspension cell transcriptome

et al. 2014

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Basal phosphoinositide-dependent phospholipase C (PI-PLC) activity controls gene expression in Arabidopsis suspension cells and seedlings. PI-PLC catalyzes the production of phosphorylated inositol and diacylglycerol (DAG) from phosphoinositides. It is not known how PI-PLC regulates the transcriptome although the action of DAG-kinase (DGK) on DAG immediately downstream from PI-PLC is responsible for some of the regulation. We previously established a list of genes whose expression is affected in the presence of PI-PLC inhibitors. Here this list of genes was used as a signature in similarity searches of curated plant hormone response transcriptome data. The strongest correlations obtained with the inhibited PI-PLC signature were with salicylic acid (SA) treatments. We confirm here that in Arabidopsis suspension cells SA treatment leads to an increase in phosphoinositides, then demonstrate that SA leads to a significant 20% decrease in phosphatidic acid, indicative of a decrease in PI-PLC products. Previous sets of microarray data were re-assessed. The SA response of one set of genes was dependent on phosphoinositides. Alterations in the levels of a second set of genes, mostly SA-repressed genes, could be related to decreases in PI-PLC products that occur in response to SA action. Together, the two groups of genes comprise at least 40% of all SA-responsive genes. Overall these two groups of genes are distinct in the functional categories of the proteins they encode, their promoter cis-elements and their regulation by DGK or phospholipase D. SA-regulated genes dependent on phosphoinositides are typical SA response genes while those with an SA response that is possibly dependent on PI-PLC products are less SA-specific. We propose a model in which SA inhibits PI-PLC activity and alters levels of PI-PLC products and substrates, thereby regulating gene expression divergently.

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HrpZ harpins from different Pseudomonas syringae pathovars differ in molecular interactions and in induction of anion channel responses in Arabidopsis thaliana suspension cells

Cited by 15 publications

References 39 publications

Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis

Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis

The cytoskeleton is disrupted by the bacterial effector HrpZ, but not by the bacterial PAMP flg22, in tobacco BY-2 cells

Salicylic acid modulates levels of phosphoinositide dependent-phospholipase C substrates and products to remodel the Arabidopsis suspension cell transcriptome

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