HPV DNA Typing of adult‐onset respiratory papillomatosis

Abstract

Respiratory papillomatosis is a recalcitrant, human papillomavirus-induced disorder of the upper airways. Using in situ hybridization and biotinylated deoxyribonucleic acid (DNA) probes, the type of human papillomavirus (HPV) in nine adults was determined both at presentation and during subsequent recurrences. Fifty-three biopsies obtained from seven men and two women over a 10-year period were examined for HPV 6, 11, 16 and 18. All patients had either type 6 or 11. One dual infection was found. Despite intensive therapy with lasers with or without alpha interferon, no elimination or change in viral type occurred. Despite identical histologies, the amount of HPV DNA present in a given patient's papillomata varied markedly. The results suggest that adult-onset respiratory papillomatosis is due to HPV 6 and/or HPV 11, and that its recurrent nature, despite therapy, is due to persistence of a viral reservoir.

Antibody response to a synthetic peptide derived from the human papillomavirus type 6/11 L2 protein in recurrent respiratory papillomatosis: Correlation between southern blot hybridization, polymerase chain reaction, and serology

“…Modern DNA hybridization techniques allowed a more sensitive detection and typing of HPV DNA in laryngeal papillomas. In situ hybridization enabled the accurate localization of the viral DNA in the lesions [Terry et al, 1987Quiney et al, 1989;Tsutsumi et al, 1989;Duggan et al, 1990;Lindeberg and Johansen, 1990;Wright et al, 1990;Rimell et al, 19921. It is now well established that nearly all respiratory recurrent papillomas are associated with the presence of HPV 6 and 11, the same viruses that are also responsible for genital condylomata acuminata (genital warts) [Gissmann and zur Hausen, 1980;Gissmann et al, 1983;Steinberg et al, 1983Steinberg et al, ,1987Steinberg et al, , 1988Mounts and Kashima, 1984;Corbitt et al, 1988;Chen and Mounts, 1989;Brandsma et al, 19901.…”

The role of HPV type in Recurrent Respiratory Papillomatosis

“…International Journal of Pediatric Otorhinolaryngology 74 (2010) [7][8][9][10][11][12][13][14] However, this is not a simple problem since the prevalence of HPV in both dormant pre-clinical and clinical conditions is variable and the influence of co-infection with other HPV types has not been fully evaluated.…”

Papilomatose laríngea recorrente: experiência de 10 anos

“…Quanto à etiologia, experimentos clínicos e estudos com microscopia óptica e eletrônica têm sugerido relação da papilomatose laríngea com infecções virais 5,6 . Somente com o desenvolvimento da biologia molecular e de diferentes técnicas de hibridização do DNA viral foi possível a demonstração desta relação, tendo sido confirmada pela técni-ca de PCR (polymerase chain reaction) 7,8,9 . Recentemente, foi possível definir que o papilloma virus humano (HPV), um DNA-vírus pertencente ao grupo de vírus lentos da família Papovaviridae é o agente causal da papilomatose laríngea.…”