HPV detection in primary intra‐oral squamous cell carcinomas – commensal, aetiological agent or contamination?

Boy, Sonja C.; Rensburg, Estrelita Janse van; Engelbrecht, Susan; Dreyer, L; Heerden, Marlene B. van; Heerden, W.F.P. Van

doi:10.1111/j.1600-0714.2006.00385.x

Cited by 75 publications

(80 citation statements)

References 26 publications

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“…A notable outlier is a study from South Africa which detected only HPV18, and not HPV16, in patients with oral cancer [23]. HPV18 was detected in a smaller percentage of oral cancers, some oral carcinomas had dual infections with HPV16/18 [51,70,96].…”

Section: Resultsmentioning

confidence: 99%

Human Papillomavirus in Non-Oropharyngeal Head and Neck Cancers: A Systematic Literature Review

Isayeva

Maswahu

et al. 2012

Head and Neck Pathol

216

210

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Perhaps one of the most important developments in head and neck oncology of the past decade is the demonstration that patients with human papillomavirus (HPV)-mediated oropharyngeal cancers have significantly improved outcomes, compared to HPV-negative counterpart patients. This has become the basis for clinical trials investigating the impact on ''treatment deintensification'' for patients with HPV-mediated oropharyngeal cancers. Unfortunately, the significance of HPV in non-oropharyngeal head and neck cancers is much less certain. Our goal is to systematically review the published data regarding the role HPV in carcinomas of the oral cavity, larynx, sinonasal tract and nasopharynx with respect to HPV detection frequency, viral activity, and association with outcome. We also present preliminary data on HPV16/18 transcriptional status in oral cavity carcinomas, as well as salivary gland neoplasia, as determined by nested reverse transcription PCR for HPV E6/E7 RNA. The weighted prevalence (WP) of HPV DNA detection in 4,195 oral cavity cancer patients is 20.2 %, (95 % CI 16.0 %, 25.2 %). HPV16 is the most common type detected. Importantly, no data currently demonstrates a significant association between the presence of HPV DNA and improved outcome. The WP of HPV DNA in 1,712 laryngeal cancer patients is 23.6 %, (95 % CI 18.7 %, 29.3 %). Similarly, no association has yet been demonstrated between HPV DNA status and outcome. The WP of HPV DNA detection in 120 sinonasal cancer patients is 29.6 % (95 % CI 17.8 %, 44.9 %), and in 154 nasopharyngeal carcinoma patients is 31.1 %, (95 % CI 20.3 %, 44.5 %). Recent preliminary data also suggests an association between HPV and certain salivary gland neoplasms. The clinical significance of these findings is unclear. The published data strongly support the need for studies on patients with oral and laryngeal carcinomas that will be powered to find any differences in clinical outcome with respect to HR-HPV and p16 overexpression.

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Section: Resultsmentioning

confidence: 99%

Human Papillomavirus in Non-Oropharyngeal Head and Neck Cancers: A Systematic Literature Review

Isayeva

Maswahu

et al. 2012

Head and Neck Pathol

216

210

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“…A large discrepancy of observed HPV prevalence in the data available can be due to analyzing samples of a particular diagnosis taken from different regions of oral cavity, thus reaching different conclusions (Mravak-Stipetic et al, 2013). The current controversy regarding the role of HPV in oral carcinogenesis is therefore justified (Boy et al, 2006). A study on HPV prevalence in oral cavity cancer (including lip, buccal mucosa, gingivobuccal complex along with anterior 2/3rd tongue) from another Indian study was reported to be around 34% (Koppikar et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Risk Stratification of Early Stage Oral Tongue Cancers Based on HPV Status and p16 Immunoexpression

Ramshankar¹,

Soundara²,

Shyamsundar³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Background: Recent epidemiological data have implicated human papilloma virus (HPV) infection in the pathogenesis of head and neck cancers, especially oropharyngeal cancers. Although, HPV has been detected in varied amounts in persons with oral dysplasia, leukoplakias and malignancies, its involvement in oral tongue carcinogenesis remains ambiguous. Materials and Methods: HPV DNA prevalence was assessed by PCR with formalin fixed paraffin embedded sections (n=167) of oral tongue squamous cell carcinoma patients and the physical status of the HPV16 DNA was assessed by qPCR. Immunohistochemistry was conducted for p16 evaluation. Results: We found the HPV prevalence in tongue cancers to be 51.2%, HPV 16 being present in 85.2% of the positive cases. A notable finding was a very poor concordance between HPV 16 DNA and p16 IHC findings (kappa<0.2). Further molecular classification of patients based on HPV16 DNA prevalence and p16 overexpression showed that patients with tumours showing p16 overexpression had increased hazard of death (HR=2.395; p=0.005) and disease recurrence (HR=2.581; p=0.002) irrespective of their HPV 16 DNA status. Conclusions: Our study has brought out several key facets which can potentially redefine our understanding of tongue cancer tumorigenesis. It has emphatically shown p16 overexpression to be a single important prognostic variable in defining a high risk group and depicting a poorer prognosis, thus highlighting the need for its routine assessment in tongue cancers. Another significant finding was a very poor concordance between p16 expression and HPV infection suggesting that p16 expression should possibly not be used as a surrogate marker for HPV infection in tongue cancers. Interestingly, the prognostic significance of p16 overexpression is different from that reported in oropharyngeal cancers. The mechanism of HPV independent p16 over expression in oral tongue cancers is possibly a distinct entity and needs to be further studied.

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“…Several methods can be used to detect HPV and different techniques with varying sensitivity and specificity could potentially influence the detection rate of HPV. In situ hybridization, immunohistochemistry and Southern blotting were utilized in the early period of HPV detection and were less sensitive than PCR (Yeudall & Campo, 1991;Frazer et al, 1993;Kay et al, 2002;Boy et al, 2006). In our study, quantitative PCR was used for the detection of HPV which is sensitive and could detect as low as 9 viral copies per reaction.…”

Section: Enumeration Of Viral Copy Number By Real-time Pcrmentioning

confidence: 99%

Detection of Human Papillomavirus in Normal Oral Cavity in a Group of Pakistani Subjects using Real-Time PCR

Gichki¹,

Buajeeb²,

Doungudomdacha³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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HPV detection in primary intra‐oral squamous cell carcinomas – commensal, aetiological agent or contamination?

Abstract: Background: High-risk human papilloma viruses (HPV) are reported to be significant independent risk factors for oral squamous cell carcinoma (OSCC). The prevalence of HPV in OSCC in a South African population sample was evaluated comparing three different HPV detection methods.

Cited by 75 publications

References 26 publications

Human Papillomavirus in Non-Oropharyngeal Head and Neck Cancers: A Systematic Literature Review

Human Papillomavirus in Non-Oropharyngeal Head and Neck Cancers: A Systematic Literature Review

Risk Stratification of Early Stage Oral Tongue Cancers Based on HPV Status and p16 Immunoexpression

Detection of Human Papillomavirus in Normal Oral Cavity in a Group of Pakistani Subjects using Real-Time PCR

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