HPV 11, 16 and 18 DNA sequences in cervical swabs from women with cervical dysplasia: prevalence and associated risk of progression

Hørding, Ulla; Daugaard, Søren; Bock, Johannes E.; Sebbelov, Anne M.; Norrild, Bodil

doi:10.1016/0028-2243(91)90043-k

Cited by 11 publications

(8 citation statements)

References 13 publications

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“…This study has an overall HPV positivity of 40% with 61% positive in the com bined severe dysplasia/CIS group. This is relatively low compared with other epidemiological studies [7][8][9], but does agree with previous findings in our laboratory [43]. The cytokeratin pattern is very heterogeneous.…”

Section: Discussioncontrasting

confidence: 35%

Cytokeratin Intermediate Filament Pattern and Human Papillomavirus Type in Uterine Cervical Biopsies with Different Histological Diagnosis

Nielsen

Hørding²,

Daugaard³

et al. 1991

Gynecol Obstet Invest

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The cytokeratin pattern and the presence of human papillomavirus (HPV) were analyzed in 53 uterine cervical biopsies. The biopsies were histologically characterized and the diagnosis ranged from normal through dysplasia to carcinoma. The cytokeratins were identified by their immunological reactivity with the monoclonal antibodies AEl and AE3. The tissue was typed for the presence of HPV types 11, 16 and 18. We have previously shown that there was no correlation between the expression of cytokeratins No. 14, 15, 16 and 19 (K14, K15, K16 and K19) and the histological diagnosis of cervical biopsies. The present study shows that the cytokeratin pattern cannot be correlated to HPV infection of the cervical tissue either.

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Section: Discussioncontrasting

confidence: 35%

Cytokeratin Intermediate Filament Pattern and Human Papillomavirus Type in Uterine Cervical Biopsies with Different Histological Diagnosis

Nielsen

Hørding²,

Daugaard³

et al. 1991

Gynecol Obstet Invest

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“…We identified 250 potentially eligible studies; 43 publications met the inclusion criteria (fig 1),6222324252627282930313233343536373839404142434445464748495051525354555657585960616263 but seven were duplicate reports of the same data 27283140505254. Of the 36 eligible studies (total of 3160 women), seven (19%) were randomised trials with suitable data in the non-experimental arm,22253339455762 16 (44%) were prospective cohort studies,6263032343536373841435155566063 and 13 (36%) were retrospective cohort studies23242942444647484953585961 (see supplementary table 2).…”

Section: Resultsmentioning

confidence: 99%

“…Of the 36 eligible studies (total of 3160 women), seven (19%) were randomised trials with suitable data in the non-experimental arm,22253339455762 16 (44%) were prospective cohort studies,6263032343536373841435155566063 and 13 (36%) were retrospective cohort studies23242942444647484953585961 (see supplementary table 2). The median follow-up was 16 months (range 3-72, interquartile range 7.6-27.4 months).…”

Section: Resultsmentioning

confidence: 99%

“…The protocols varied greatly between the studies, and of the 32 studies reporting the protocol, the definite criteria for colposcopic evaluation or histological sampling during the follow-up could not be defined in 11 studies. In the prospective, low risk of bias studies,22252630313335363738434751565763 the most typical protocols included cytology and colposcopy every three to four months, with routine biopsies or biopsies when progression was suspected.…”

Section: Resultsmentioning

confidence: 99%

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Clinical course of untreated cervical intraepithelial neoplasia grade 2 under active surveillance: systematic review and meta-analysis

Tainio

Athanasiou

Tikkinen

et al. 2018

BMJ

264

242

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ObjectiveTo estimate the regression, persistence, and progression of untreated cervical intraepithelial neoplasia grade 2 (CIN2) lesions managed conservatively as well as compliance with follow-up protocols.DesignSystematic review and meta-analysis.Data sourcesMedline, Embase, and the Cumulative Index to Nursing and Allied Health Literature (CINAHL) from 1 January 1973 to 20 August 2016.Eligibility criteriaStudies reporting on outcomes of histologically confirmed CIN2 in non-pregnant women, managed conservatively for three or more months.Data synthesisTwo reviewers extracted data and assessed risk of bias. Random effects model was used to calculate pooled proportions for each outcome, and heterogeneity was assessed using I2 statistics.Main outcome measuresRates of regression, persistence, or progression of CIN2 and default rates at different follow-up time points (3, 6, 12, 24, 36, and 60 months).Results36 studies that included 3160 women were identified (seven randomised trials, 16 prospective cohorts, and 13 retrospective cohorts; 50% of the studies were at low risk of bias). At 24 months, the pooled rates were 50% (11 studies, 819/1470 women, 95% confidence interval 43% to 57%; I2=77%) for regression, 32% (eight studies, 334/1257 women, 23% to 42%; I2=82%) for persistence, and 18% (nine studies, 282/1445 women, 11% to 27%; I2=90%) for progression. In a subgroup analysis including 1069 women aged less than 30 years, the rates were 60% (four studies, 638/1069 women, 57% to 63%; I2=0%), 23% (two studies, 226/938 women, 20% to 26%; I2=97%), and 11% (three studies, 163/1033 women, 5% to 19%; I2=67%), respectively. The rate of non-compliance (at six to 24 months of follow-up) in prospective studies was around 10%.ConclusionsMost CIN2 lesions, particularly in young women (<30 years), regress spontaneously. Active surveillance, rather than immediate intervention, is therefore justified, especially among young women who are likely to adhere to monitoring.Systematic review registrationPROSPERO 2014: CRD42014014406.

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“…However, it is generally believed that early lesions with HPV 16 do progress more frequently than virus-free lesions (3,12,28). This observation makes it desirable to identify the risk group as soon as the early lesions appear.…”

Section: Discussionmentioning

confidence: 99%

The prevalence of human papillomavirus in cervical lesions with koilocytosis and/or CINI

Iversen¹,

Duun

Sebbelov³

et al. 1992

APMIS

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The prevalence of human papillomavirus in cervical lesions with koilocytosis and/or CIN I. APMIS 100: 280-286, 1992. Thirthy-one patients with koilocytosis and/or concomitant CIN I were analysed for the presence of HPV types 11, 16 and 18 by in situ hybridization and Southern blot analysis. The prevalence of HPV was 48%) and 55%, respectively, when measured by the two methods and among the HPV positive, HPV 11 and 16 were present in 47% and 60%, respectively, whereas HPV 18 was not found.Clinical epidemiological studies support the hypothesis that precancerous and cancerous lesions of the uterine cervix are related to human papillomavirus (HPV) infection (1,9,16,17, 33, 34). Distinct subtypes of HPV have been demonstrated in 60-100% of precancerous and malignant lesions of the cervix, and an essential event in the malignant transformation may be the integration of the viral genome into the chromosomal DNA (7,24,27, 32). More than 60 HPV types and subtypes have been cloned and characterized (6). At present more than 20 different HPV types have been associated with infections in the genital mucosa. HPV 6 and 11 are most frequently found in genital condylomas and in lower grade cervical intraepithelial neoplasia (CIN I) (reviewed by Bosch & Munoz (1). The HPV types 16, 18, 31, 33, 35, 45, 51, and 52 are most often associated with higher grades of dysplasia (CIN I1 and CIN III/CIS) and invasive carcinoma of the uterine cervix (1). The prevailing types are HPV 16 and 18, which can be demonstrated in 60-100% of specimens with CIN 11, 111 and invasive cancer (1, 32). Typing of HPV in early lesions is believed to be of prognostic value and allows identification of possible risk groups. It is therefore desirable to develop diagnostic methods which are reliable, fast and inexpensive. Genital lesions are detected by clinical examination and by analysis of histological and cytological specimens, but identification of HPV types is only possible by nucleic acid hybridization or polymerase chain reaction (PCR) (14,18, 32).Brigati, D. J., Myerson, D. & Leary, J. J.: Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes. J. Virol. 126: [32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50] 1983. Campion, M. J., McCance, D. J., Cuzick, J. & Singer, A.; Progressive potential of mild cervical atypia: prospective cytological, colposcopic, and virological study. Lancet Aug 2: 237-240, 1986. Caussy , D., Marrett, L. D., Worth, A. J., McBride, M. & Rawls, W E.: Human papillomavirus and cervical intraepithelial neoplasia in women who subsequently had invasive cancer. Can. Med. As-Collins, J. E., Jenkins, D. & McCance, D. J.: Detection of human papillomavirus DNA sequences by in situ DNA-DNA hybridization in cervical intraepithelial neoplasia and invasive carcinoma: a retrospective study. J. Clin. Pathol. DeVilliers, E. M.: Heterogeneity of the human 135-151, 1989. SOC.

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HPV 11, 16 and 18 DNA sequences in cervical swabs from women with cervical dysplasia: prevalence and associated risk of progression

Cited by 11 publications

References 13 publications

Cytokeratin Intermediate Filament Pattern and Human Papillomavirus Type in Uterine Cervical Biopsies with Different Histological Diagnosis

Cytokeratin Intermediate Filament Pattern and Human Papillomavirus Type in Uterine Cervical Biopsies with Different Histological Diagnosis

Clinical course of untreated cervical intraepithelial neoplasia grade 2 under active surveillance: systematic review and meta-analysis

The prevalence of human papillomavirus in cervical lesions with koilocytosis and/or CINI

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