Cytokeratin Intermediate Filament Pattern and Human Papillomavirus Type in Uterine Cervical Biopsies with Different Histological Diagnosis

Abstract

The cytokeratin pattern and the presence of human papillomavirus (HPV) were analyzed in 53 uterine cervical biopsies. The biopsies were histologically characterized and the diagnosis ranged from normal through dysplasia to carcinoma. The cytokeratins were identified by their immunological reactivity with the monoclonal antibodies AEl and AE3. The tissue was typed for the presence of HPV types 11, 16 and 18. We have previously shown that there was no correlation between the expression of cytokeratins No. 14, 15, 16 and 19 (K14, K15, K16 and K19) and the histological diagnosis of cervical biopsies. The present study shows that the cytokeratin pattern cannot be correlated to HPV infection of the cervical tissue either.

A three-dimensional organotypic culture of the human uterine exocervix for studying mucosal epithelial differentiation and migrating leukocytes

“…Prior to culture, both the squamous epithelium and the stroma showed normal histology and expression patterns of the cellular markers examined, as reported for p63 (Di Como et al, 2002), Ki‐67 (Payne et al, 1996), involucrin (Serra et al, 1990), AE3 (Nielsen et al, 1991), ER (Kanai et al, 1998), vimentin (Puts et al, 1986), CD45 (Stern et al, 1998), and CD31 (Dellas et al, 1997).…”

Increased Expression of Cytokeratins 14, 18 and 19 Correlates with Tumor Progression in the Uterine Cervix

“…This upregulation or increased immunoreactivity of CKs specific for basal cells may indicate increased cell proliferation or the invasive po tential of the tumors analyzed [28]. In contrast downregulation of CK 13 during tumor progression in the uter ine cervix may be attributed to the differentiation poten tial of the lesion and may be used as a marker in assess ing the grade of the lesion, in addition to the routine histopathological criteria.…”