HPTLC, with UV and MS detection, and preparative-layer chromatography for analysis and purification of synthesis products

Planar Chromatography

“…The following are additional studies involving slit scanning densitometry in the absorbance−reflectance mode: determination of nitrendipine in tablets (scanning at 335 nm) ; determination of lipids and phospholipids in the medicinal leech Hirudo medicinalis maintained on different diets (scanning at 610 and 370 nm) ; detection of the iridoids of Satchys species growing in Hungary (detection with Erlich’s spray reagent and scanning at 540 nm) ; monitoring the dose of florfenicol in medicated salmon feed (scanning at 223 nm) ; standardization of the Ayurvedic medicinal plant Piper longum (scanning at 260 nm) ; evaluation of the stereochemically peculiar 2D separation of 2-arylpropionic acids by chiral TLC (concentration profiles recorded by scanning at 210 nm) ; separation of indole alkaloids from Rauvolfia serpentina roots (detection by dipping in Dragendorff reagent and scanning at 520 nm) ; quantification of pantoprazole sodium sesquihydrate and domperodone in tablets (scanning at 286 nm) ; determination of itopride hydrochloride in a pharmaceutical preparation and bulk drug (scanning at 230 nm) ; analysis and purification of synthesis products (scanning at 280 nm, AP-MALDI-MS, and PLC) ; quantitative screening of the myricitrin content of crude methanolic extracts of Acer species leaves (scanning at 254 nm) ; simultaneous determination of diclofenac sodium and paracetamol in a pharmaceutical preparation and bulk drug powder (scanning at 260 nm) ; quantitative analysis of selected phenolic acids for standardization of Propolis concentrates (scanning at 320 nm) ; quantification of phenobarbital in a dosage form (scanning at 210 nm) ; monoterpenoid alkaloid assay in areal tissues of Catharanthus roseus (scanning at 280 nm) ; and determination of curcuminoids from Curcuma longa (scanning at 366 nm) .…”

“…Vertical development in a saturated chamber with chloroform or dichloromethane mobile phase required 60 min, after which the zones were detected under UV light and compounds recovered by scraping and elution using a device constructed from a Finntip 1000 and Bio-Inert Microsep II membrane. Purity of the products was established by analytical HPTLC−UV and atmospheric pressure (AP)-MALDI-MS .…”

Application of electrospray ionization product ion spectra for identification with atmospheric pressure matrix‐assisted laser desorption/ionization mass spectrometry – a case study with seized drugs

“…Salo et al . used ultrathin‐layer chromatography plates and high performance thin‐layer chromatography plates coupled to AP‐MALDI‐MS for the detection small drug molecules directly from the plates . Pihlainen et al .…”

“…Salo et al used ultrathin-layer chromatography plates and high performance thin-layer chromatography plates coupled to AP-MALDI-MS for the detection small drug molecules directly from the plates. [24,25] Pihlainen et al used both DIOS-MS and AP-MALDI-MS with an ion trap for the analysis of seized drugs. [26] Wu et al studied the coupling of the single-drop microextraction technique with AP-MALDI-MS for example in the analysis of drugs in urine, [27] and nortriptyline and quinine in urine and plasma.…”