HPTLC Marker-based Analysis of Madhuram, a Pre-diabetic Proprietary Poly Herbal

Abstract

Rising global herbal drug trade and rampant availability of herbal drug combinations are a cause for concern with regards to its safety and efficacy in view of their indiscriminate use. Thus, their quality control is now a mandatory regulatory requirement both for over the counter sale and towards integrating popularly prescribed herbals-both classical and proprietary into modern clinical practice. Despite being a major hub for medicinal plants with clinically proven efficacy in traditional medical practice, India is yet to exploit its potential in keeping with the growing international market for plant based products. Lack of standardization protocols and thus regulatory hurdles are major constraints for even popularly prescribed Indian herbals from reaching global markets. Development of analytical profiles is thus an essential prerequisite in herbal drug standardization and methods developed for single molecule drugs are not largely adaptable to multi constituent plant drugs.In this scenario, high-performance thin-layer chromatography (HPTLC) is emerging as a preferred analytical tool for standardizing single herbs and polyherbal formulations [1] with better resolution and accuracy [2] . The WHO stresses on the importance of the quantitative and qualitative methods of characterizing samples, quantification of biomarkers and/or chemical markers and development of fingerprint profiles. There are several challenges to be overcome in the HPTLC characterization of herbal products. The chemical profile of the constituent herbs in herbal medicinal products (HMPs) may vary due to several factors, the important ones being the plant identity and seasonal variation, the ecotypic, genotypic and chemotypic variations, processing and other factors. As for HMPs, establishment of specific quality control method is very difficult because of their complex nature [3] . HPTLC Marker-based Analysis of

Determination of gymnemic acid level in Gymnema inodorum leaves using multiple reaction monitoring mass spectrometry

“…Since the derivatives of GA are comprised of the GM skeleton, the quantity of GM is equivalent to that of GA. The detection of GM can be carried out by using thin layer chromatography [9,10], high performance liquid chromatography with UV or mass detectors [11 -13]. In addition, Kamble et al [14] showed that the method of HPLC-MS electrospray ionization with multiple reaction monitoring was a high throughput technique for measuring GM aglycone of total GA derivatives in G. sylvestre extract and commercial products.…”