HPTLC finger printing profile and evaluation of in vitro antidiabetic potential of medicinally important plant Cassia obtusa L. (Caesalpiniaceae)

Thenmozhi, K; Senguttuvan, Jamuna; Karthika, K.; Manian, S.; Paulsamy, S.; Chitravadivu, C.

doi:10.7324/japs.2015.50312

Cited by 5 publications

(4 citation statements)

References 3 publications

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“…Diabetes mellitus is a complex of metabolic diseases characterized by hyperglycemia caused by problems in insulin secretion, insulin dysfunction or both (Thenmozhi et al, 2015). The search for safer and more effective anti-diabetic agents without any side effects has been of interest to research.…”

Section: Introductionmentioning

confidence: 99%

In vitro anti-diabetic activity of Tribulus terrestrisL. fruits extracts

AlKhaldi

Daghestani

Al-Haddad

2019

NFS

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Purpose The purpose of this paper is to evaluate the inhibition activity of Tribulus terrestris L. (T. terrestris) fruits extracts with solvents of increasing polarity against α-glucosidase and α-amylase, and to determine the inhibition mode of the most effective extract against both enzymes. Design/methodology/approach Hexane, acetone, ethanol and aqueous extracts of T. terrestris fruits were prepared using ultrasonic sequential extraction and analyzed for their α-amylase and α-glucosidase inhibitory activities by specific assay for each enzyme. The modes of inhibitions were detected using Lineweaver–Burk plots. Findings T. terrestris fruits extracts showed inhibition activity against α-glucosidase and α-amylase which was in the dose-dependent manner. Hexane extract had the highest α-glucosidase inhibition activity (IC50 = 27.28 μg/ml, p = 0.003), followed by acetone and ethanol extracts (IC50 = 60.58 μg/ml and IC50 = 84.21 μg/ml, respectively). The inhibition mode of hexane extract was noncompetitive. While acetone extract showed the highest inhibition activity against α-amylase (IC50 = 6.18 mg/ml, p = 0.002), hexane and ethanol extracts showed no significant difference (IC50 = 13.04 mg/ml and IC50 = 14.20 mg/ml, respectively, p = 0.09). The inhibition mode of acetone extract was competitive. Originality/value T. terrestris fruits extracts had strong inhibition activity against α-glucosidase and α-amylase, and they can be used as a promising anti-diabetic agent.

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Section: Introductionmentioning

confidence: 99%

In vitro anti-diabetic activity of Tribulus terrestrisL. fruits extracts

AlKhaldi

Daghestani

Al-Haddad

2019

NFS

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“…The HPTLC method is simple, rapid, accurate, reproducible, selective and economic, can be used for quality control analysis and quantitative determination of the plant material. The qualitative determination of HPTLC profiles from medicinal plants is a fast expanding field of research to identify the chemical profiles with curative properties 28 . HPTLC studies have shown that it is more resourceful than ordinary TLC methods, as the spots are well resolved.…”

Section: Resultsmentioning

confidence: 99%

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2019

IJPSR

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To establish the preliminary phytochemical screening and fingerprint profile of stem bark Extracts of Maytenus emarginata by using highperformance thin layer chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done, physical constants were evaluated, and HPTLC studies were carried out. CAMAG make HPTLC system equipped with Linomat 5 applicator, TLC scanner 3, server vision CATS-server PH, version 2.5.18072.1 software were used. Results: Preliminary phytochemical screening of the extract showed the presence of flavonoids, glycosides, tannins, alkaloids, terpenoids, phenolic compounds, proteins, reducing sugars, fats, and oils. The fingerprint analysis of petroleum ether extract showed four peaks; chloroform extract showed four peaks and methanolic extract showed six peaks in 10 μl of the sample analyzed. Conclusions: It can be concluded that HPTLC fingerprint analysis of Petroleum ether, chloroform and methanolic extract of stem bark of Maytenus emarginata can be used as a diagnostic tool for the correct identification of the plant and it is useful as a phytochemical marker and also a good estimator of genetic variability in plant populations.

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“…Plants are provided with secondary metabolites such as alkaloids, flavonoids, tannins, phenols and saponins which are also known as bioactive compounds and these bioactive compounds possess different biological activities which include antibacterial, antioxidant, anti-inflammatory, anticancer and antidiabetic and especially flavonoids already reported to treat type 2 diabetes [57][58][59][60] . In the recent years medicinal plants and their products have been investigated for potential benefits in the management of type 2 diabetes 61,62 .…”

Section: Resultsmentioning

confidence: 99%

Untitled

2017

IJPSR

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Objective of the present work was to evaluating the antidiabetic property of Kandelia candel and Rhizophora apiculata extracts by using in vitro assays. The serial exhaustive extraction was carried out with a series of solvents: chloroform, ethyl acetate, methanol, ethanol and water with increasing polarity using Soxhlet apparatus. The concentrated and dried extracts were evaluated for antidiabetic activity by employing standard in vitro techniques (Alpha-amylase and glucose uptake assay using yeast model). In vitro antidiabetic studies show that in case of Kandelia candel methanol extract showed higher antidiabetic activity where as incase of Rhizophora apiculata aqueous extract exhibited significant activity when compared to other solvent extracts. Results confirm that aqueous extract of Rhizophora apiculata exhibited highest antidiabetic activity among all extracts. This study provides scientific evidence that the leaves of Kandelia candel and Rhizophora apiculata have anti-diabetic efficacy. However further comprehensive chemical and pharmacological investigation should be carried out to isolate the active compounds and appropriate elucidation of its mechanism of action and it helps in the development of new pharmaceuticals to treat Diabetes mellitus. INTRODUCTION: Diabetes mellitus (DM) is a non-communicable disease often genetic in nature but can be developed due to life style and habits 1, 2. DM is a set of metabolic disorder with a common feature of high blood glucose level. It is characterized by increased blood glucose, triglyceride and hypo insulinemia that may lead to decrease in both insulin action and secretion 3, 4. Diabetes mellitus ranks highly with the top ten disorders which cause mortality throughout the world and is affecting approximately 30% of the worldwide population 5-7 .

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HPTLC finger printing profile and evaluation of in vitro antidiabetic potential of medicinally important plant Cassia obtusa L. (Caesalpiniaceae)

Cited by 5 publications

References 3 publications

In vitro anti-diabetic activity of Tribulus terrestrisL. fruits extracts

In vitro anti-diabetic activity of Tribulus terrestrisL. fruits extracts

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